Human irisin ELISA kit

Code CSB-EQ027943HU
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name irisin
Abbreviation irisin
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 3.12 ng/mL-200 ng/mL
Sensitivity 0.78 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Others
Assay Principle quantitative
Measurement Sandwich
Intra-assay Precision (Precision within an assay): CV%<8%        
Three samples of known concentration were tested twenty times on one plate to assess.    
Inter-assay Precision (Precision between assays): CV%<10%        
Three samples of known concentration were tested in twenty assays to assess.      
To assess the linearity of the assay, samples were spiked with high concentrations of human irisin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
  Sample Serum(n=4)    
1:1 Average % 90    
Range % 84-96    
1:2 Average % 97    
Range % 91-103    
1:4 Average % 103    
Range % 97-109    
1:8 Average % 89    
Range % 83-95    
The recovery of human irisin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
Sample Type Average % Recovery Range    
Serum (n=5) 100 94-106    
EDTA plasma (n=4) 106 101-112    
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
ng/ml OD1 OD2 Average Corrected    
200 2.690 2.752 2.721 2.616    
100 2.402 2.371 2.387 2.282    
50 1.965 1.853 1.909 1.804    
25 1.355 1.247 1.301 1.196    
12.5 0.764 0.735 0.750 0.645    
6.25 0.495 0.464 0.480 0.375    
3.12 0.251 0.266 0.259 0.154    
0 0.106 0.103 0.105      
and FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days


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