Human leukotriene E4,LT-E4 ELISA Kit

Code CSB-E05176h
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name leukotriene E4,LT-E4
Abbreviation LT-E4
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 0.625 ng/mL-40 ng/mL
Sensitivity 0.156 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Biochemicals
Assay Principle quantitative
Measurement Sandwich
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
To assess the linearity of the assay, samples were spiked with high concentrations of human LT-E4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 88  
Range % 83-95  
1:2 Average % 95  
Range % 91-98  
1:4 Average % 95  
Range % 88-100  
1:8 Average % 98  
Range % 91-103  
The recovery of human LT-E4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 85 81-93  
EDTA plasma (n=4) 96 93-105  
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected  
40 2.975 2.962 2.969 2.819  
20 2.537 2.574 2.556 2.406  
10 1.646 1.769 1.708 1.558  
5 0.819 0.887 0.853 0.703  
2.5 0.471 0.491 0.481 0.331  
1.25 0.233 0.251 0.242 0.092  
0.625 0.211 0.201 0.206 0.056  
0 0.148 0.152 0.150    
and FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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