Human melanin ELISA kit

Product Details

Citations

Customer Reviews and Q&A

Citations (4) Protocol

Code	CSB-E14051h
Size	96T,5×96T,10×96T
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Product Details
Citations (4)
Customer Reviews and Q&A

Target Name

melanin

Abbreviation

melanin

Species

Homo sapiens (Human)

Sample Types

serum

Detection Range

12.5 pg/mL-1000 pg/mL

Sensitivity

15.6 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Others

Assay Principle

quantitative

Measurement

Competitive

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human melanin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	94
	Range %	89-102
1:2	Average %	95
	Range %	91-100
1:4	Average %	94
	Range %	92-97
1:8	Average %	95
	Range %	90-101

Recovery

The recovery of human melanin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	92-105
EDTA plasma (n=4)	96	91-103

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average
1000	0.145	0.147	0.146
375	0.276	0.286	0.281
187.5	0.402	0.413	0.408
62.5	0.553	0.562	0.558
12.5	1.051	1.084	1.068
0	2.453	2.567	2.510

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.

Description

This Human melanin ELISA Kit was designed for the quantitative measurement of Human melanin protein in serum. It is a Competitive ELISA kit, its detection range is 12.5 pg/mL-1000 pg/mL and the sensitivity is 15.6 pg/mL.

Exosomes Derived from Hypertrophic Scar Fibroblasts Suppress Melanogenesis in Normal Human Epidermal Melanocytes HS Cui,International Journal of Molecular Sciences,2024
YAP prevents senescence of dermal fibroblast and inhibits melanogenesis via paracrine effect of DKK1 T Li,Experimental dermatology,2024
Skin Anti-Aging Potential through Whitening and Wrinkle Improvement Using Fermented Oil Derived from Hermetia illucens Larvae D Hwang,International journal of molecular sciences,2024
Comparison of the global gene expression profiles in the bovine endometrium between summer and autumn. Sakumoto R. et al,J Reprod Dev,2015

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

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■ Q&A

Which type of melanin do you dose?The three types have different molecular structures. how can we be sure that the antibodies recognize all the target? And so my question is how can we be sure that we dose the whole melanin? Thanks for your help!

Thanks for your inquiry. Our kit detects the general melanin and has no detailed types. Small molecules are also different in vivo and in vitro. The body may be combined with other proteins or small molecules to aggregate, and our box detects such a total form. In addition, the small molecule is the reference https://pubchem.ncbi.nlm.nih.gov/compound/6325610#section=Top design.Pls let me know if you have any further questions. Thank you.

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