Human microtubule-associated protein 2,MAP-2 ELISA Kit

Instructions
Code CSB-E09330h
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name microtubule-associated protein 2
Alternative Names DKFZp686I2148 ELISA Kit; MAP 2 ELISA Kit; MAP dendrite specific ELISA Kit; MAP-2 ELISA Kit; MAP2 ELISA Kit; MAP2A ELISA Kit; MAP2B ELISA Kit; MAP2C ELISA Kit; Microtubule associated protein 2 ELISA Kit; Microtubule-associated protein 2 ELISA Kit; MTAP2_HUMAN ELISA Kit
Abbreviation MAP2
Uniprot No. P11137
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 0.156 ng/mL-10 ng/mL
Sensitivity 0.039 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human MAP-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %92
Range %86-95
1:2Average %104
Range %100-107
1:4Average %92
Range %87-95
1:8Average %97
Range %91-102
Recovery
The recovery of human MAP-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9389-96
EDTA plasma (n=4)9790-99
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
101.626 1.613 1.620 1.528
51.194 1.176 1.185 1.093
2.50.848 0.832 0.840 0.748
1.250.602 0.598 0.600 0.508
0.6250.428 0.412 0.420 0.328
0.3120.264 0.286 0.275 0.183
0.1560.158 0.154 0.156 0.064
00.093 0.091 0.092  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Data

Function The exact function of MAP2 is unknown but MAPs may stabilize the microtubules against depolymerization. They also seem to have a stiffening effect on microtubules.
Gene References into Functions
  1. study suggests that miR-484 and MAP2 can be utilized as predictors for the clinical diagnosis and prognosis of glioma PMID: 29480405
  2. Results highlight the importance of splicing alteration in HD and suggest that MAP2 alteration contributes to dendritic atrophy. PMID: 27098187
  3. The expression of MAP2 and SYP proved most relevant to neurocognitive functioning in HIV-infected patients. PMID: 26637429
  4. In patients with HD, a panel using calretinin and peripherin with or without MAP-2 may be most helpful in identifying transition zones PMID: 26469323
  5. Transcriptional upregulation of microtubule-associated protein 2 is involved in the protein kinase A-induced decrease in the invasiveness of glioma cells. PMID: 26014048
  6. Study reveals the presence of two selected neuronal markers, MAP-2 and SYP mRNAs and protein expression, in eutopic endometrium and in endometriotic lesions. PMID: 26604067
  7. Findings demonstrate that microtubule-associated protein 2-immunoreactivity loss is closely linked to dendritic spine pathology in schizophrenia. PMID: 25818630
  8. This is the first report to analyze the correlation between ET-1-induced miRNA 133a overexpression in proteinuria resulting in MRP2 downregulation, which is a contributing factor for renal cytotoxicity. PMID: 25871823
  9. the difference of only two amino acids in tau and MAP2 sequences may determine their different fates in tauopathies PMID: 26134402
  10. MAP2 expression is affected by actomyosin contractility during nanotopography-directed neuronal differentiation of human embryonic stem cells PMID: 25682157
  11. Prolactin significantly increased the gene expression and protein levels of MAP2 in SK-N-SH neuroblastoma cells, while no significant changes were observed in U-87MG glioblastoma cells. PMID: 25629269
  12. SNCG, MAP2, SDF-1 and CXCR4 may play an important role in the carcinogenesis, progression, invasion and metastasis of gastric adenocarcinoma. PMID: 25400739
  13. MAP2 expression in ovarian teratomas does not appear to have a role in predictive the development of anti-NMDAR encephalitis. PMID: 25569473
  14. PP2A/striatin complex modulates microtubule dynamics by regulating MAP2 phosphorylation PMID: 25497017
  15. MAP 2 is a moderately sensitive marker of haemangioblastoma PMID: 24977729
  16. MAP2 may play a significant role in neurotoxicity in the Alzheimer's disease brain despite the absence of MAP2-aggregates. PMID: 24587039
  17. Study suggests that many of the morphological changes detected in children's brainstems regarding distributions of HIF-1alpha and MAP-2 could be due to age differences, but in some regions these distributions are more dependent on the hypoxic conditions PMID: 24780770
  18. Altered MAP2 and tau expression in mesial temporal lobe epilepsy (MTLE) and decreased tau expression in MTLE with psychosis were found. PMID: 24069608
  19. MAP-2 and nestin participate in the regulation of the development of gastric tissues in human embryos. PMID: 22985576
  20. Dysfunction of MAP2/tau family leads to disruption of microtubule structure and impairment of axonal transport, and eventually triggers apoptosis in neurons, which becomes an essential pathway for prion to induce the neuropathology. PMID: 22874672
  21. The protein phosphatase PP2A/Balpha binds to the microtubule-associated proteins Tau and MAP2 at a motif also recognized by the kinase Fyn. PMID: 22403409
  22. Data indicate that the decline of MAP2 is a common phenomenon in transmissible spongiform encephalopathies (TSEs), which seems to occur at an early stage of incubation period. PMID: 22272295
  23. Increased MAP2 expression in malignant melanoma cells significantly inhibits tumor cell growth and neoplasm invasiveness, and induces apoptosis and cell cycle arrest. PMID: 20100193
  24. The results of this study confirmed previous published data showing a schizophrenia-associated decrease in MAP2 in area 9 with no change in area 17 and no change in Huntington chorea. PMID: 20092829
  25. Hyperactivation of BRAF-MEK signaling activates MAP2 expression in melanoma cells by two independent mechanisms, promoter demethylation or down-regulation of neuronal transcription repressor HES1. PMID: 19880519
  26. MAP2 expression in astrocytomas is due predominantly to low molecular weight isoforms, evident in astrocytes as well as neurons; focal expression of hmw-MAP2 suggests that neuronal antigens may be expressed, particularly in high grade astrocytomas. PMID: 11922706
  27. role of small cluster of charged residues in controlling filament morphology PMID: 12023276
  28. beta-chains of tubulin and MAP-2 are expressed functionally in SCLC cell lines in association with the development of dendrite-like processes on PC-9 / ECM substrate PMID: 12079519
  29. These results provide strong evidence suggesting that MAP2c and tau stabilize microtubules by binding along individual protofilaments, possibly by bridging the tubulin interfaces. PMID: 12082079
  30. Immunoreactivity of MAP-2 has been demonstrated in most neuroendocrine and neuroectodermal related neoplasms such as small cell carcinoma, large cell neuroendocrine carcinoma, carcinoid tumor of the lung. PMID: 12605092
  31. binding of dehydroepiandrosterone to MAP2C involves specific hydrogen bonds that orient the steroid into the pocket PMID: 12775713
  32. The differential distribution of various epitopes suggests that the presence of the Phospho-Ser(262) epitope of tau either accelerates the transition form pre-tangle to tangle, or appears later than the other epitopes in the process of tangle formation. PMID: 12927759
  33. over-expression of microtubule-associated protein 2 is associated with oral squamous cell carcinoma PMID: 14604896
  34. These data point towards MAP2 as valuable diagnostic tool for pattern recognition and differential diagnosis of low-grade neuroepithelial tumors. PMID: 15146346
  35. Decreased spinophilin but unchanged MAP2 expression provides molecular evidence for a hippocampal dendritic pathology in schizophrenia that preferentially affects the spines PMID: 15465982
  36. determined that Fyn phosphorylated MAP-2c on tyrosine 67 PMID: 15536091
  37. MAP2 is involved in the association of endoplasmic reticulum membranes with microtubules. PMID: 15623521
  38. Independent of one another MAP-2c and Fyn are able to induce process outgrowth and in concert can initiate and enhance process outgrowth in an additive manner. PMID: 16145685
  39. MAP-2 is expressed in the cytoplasm of non-tumorous adenohypophysial cells as well as of various pituitary adenoma types. PMID: 16195780
  40. Tat action on MAP2 stability involved Tat-mediated translocation of the proteasome to the site of microtubule filaments. PMID: 16611822
  41. Notch signaling, which is implicated in melanoma progression, and Hairy and Enhancer of Split (HES1) play a role in MAP2 gene regulation during melanoma progression. PMID: 16916793
  42. Thus, our findings have not only identified a novel role of MAP2 in non-neuronal cells, but also provided the first implication of MAP2 in malignant oral cancer tissues. PMID: 18073143
  43. Data show that the Sudden infant death syndrome group and hypoxic/ischemic group exhibites a general reduction in the number of MAP2 reactive neurons in comparison with the non-hypoxic/ischemic injury group. PMID: 19009302
  44. MAP-2 immunoexpression in gliomatosis cerebri PMID: 19309409
  45. In contrast with the findings of previous studies, our data indicate that MAP-2 is a moderately positive predictor of the progression of superficial spreading melanomas. PMID: 19369632
  46. MAP-2 is a sensitive and specific marker of neuroblastoma, both in the primary tumor and bone marrow biopsy settings. PMID: 19701075

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Subcellular Location Cytoplasm, cytoskeleton, Cell projection, dendrite
Database Links

HGNC: 6839

OMIM: 157130

KEGG: hsa:4133

STRING: 9606.ENSP00000353508

UniGene: Hs.368281

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