Human midregional pro-atrial natriuretic peptide (MR-proANP) ELISA kit

Code CSB-EQ028056HU
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details


This Human MR-proANP ELISA Kit was designed for the quantitative measurement of Human MR-proANP protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 39 pmol/L-2500 pmol/L and the sensitivity is 9.8 pmol/L.

Target Name midregional pro-atrial natriuretic peptide (MR-proANP)
Abbreviation MR-proANP
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 39 pmol/L-2500 pmol/L
Sensitivity 9.8 pmol/L
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Others
Assay Principle quantitative
Measurement Sandwich
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
To assess the linearity of the assay, samples were spiked with high concentrations of human MR-proANP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 94  
Range % 85-97  
1:2 Average % 100  
Range % 94-105  
1:4 Average % 90  
Range % 83-94  
1:8 Average % 97  
Range % 91-101  
The recovery of human MR-proANP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 95 89-98  
EDTA plasma (n=4) 102 92-107  
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pmol/L OD1 OD2 Average Corrected  
2500 2.155 1.988 2.072 1.926  
1250 1.697 1.627 1.662 1.516  
625 1.160 1.088 1.124 0.978  
312 0.692 0.708 0.700 0.554  
156 0.414 0.425 0.420 0.274  
78 0.283 0.277 0.280 0.134  
39 0.197 0.191 0.194 0.048  
0 0.148 0.144 0.146    
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
and FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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I'm just wondering what the difference between MR-proANP and proANP or NT-proANP is. Do you know that?


ANP is secreted by the atrium and secreted by the pro-form of proANP. MR-proANP and NT-proANP are 2 different parts. The peptide in the atrial natriuretic peptide precursor is MR-proANP. Atrial natriuretic peptide precursor N-terminal peptide is NT-proANP. MR is the middle peptide of proANP and NT is the amino (N) terminal peptide.

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