Human neuregulin-4 (NRG4) ELISA kit

Instructions
Code CSB-EL016080HU
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name neuregulin-4 (NRG4)
Alternative Names DKFZp779N0541 ELISA Kit; DKFZp779N1944 ELISA Kit; Heregulin 4 ELISA Kit; HRG4 ELISA Kit; Neuregulin 4 ELISA Kit; Neuregulin-4 ELISA Kit; NRG-4 ELISA Kit; NRG4 ELISA Kit; NRG4_HUMAN ELISA Kit; pro-neuregulin-4; membrane-bound isoform ELISA Kit; Pro-NRG4 ELISA Kit
Abbreviation NRG4
Uniprot No. Q8WWG1
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 125 pg/mL-8000 pg/mL
Sensitivity 31.25 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Neuroscience
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human NRG4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %90
Range %86-95
1:2Average %97
Range %91-105
1:4Average %95
Range %90-101
1:8Average %85
Range %82-89
Recovery
The recovery of human NRG4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9087-96
EDTA plasma (n=4)9894-103
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
80002.335 2.256 2.296 2.102
40001.648 1.768 1.708 1.514
20001.191 1.201 1.196 1.002
10000.812 0.823 0.818 0.624
5000.588 0.596 0.592 0.398
2500.475 0.484 0.480 0.286
1250.367 0.381 0.374 0.180
00.193 0.195 0.194  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 7-14 working days

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Target Background

Function
(From Uniprot)
Low affinity ligand for the ERBB4 tyrosine kinase receptor. Concomitantly recruits ERBB1 and ERBB2 coreceptors, resulting in ligand-stimulated tyrosine phosphorylation and activation of the ERBB receptors. Does not bind to the ERBB1, ERBB2 and ERBB3 receptors (By similarity).
Gene References into Functions
  1. Nrg4 is highly and preferentially expressed in healthy adipocytes, while its expression was substantially reduced in obesity. Nrg4 activated endothelial angiogenic functions and angiogenesis both in vitro and in vivo. PMID: 29902456
  2. Serum NRG4 values were significantly elevated in the Gestational Diabetes Mellitus (GDM) group compared to the control group and is associated with metabolic parameters of GDM. PMID: 29282998
  3. circulating Nrg4 concentrations are inversely associated with subclinical atherosclerosis in obese adults; circulating Nrg4 might play a role in identifying patients at high risk for cardiovascular disease PMID: 27819316
  4. The circulating Nrg4 level is elevated in the prediabetic and diabetic patients compared to control and is an independent risk factor associated with diabetes. PMID: 27862843
  5. High serum Nrg4 levels are associated with type 2 diabetes mellitus. PMID: 27329015
  6. NRG4 and its receptor ErbB4 are present in human breast milk and developing human intestine, respectively. Thus, NRG4-ErbB4 signaling may be a novel pathway for therapeutic intervention or prevention in NEC. PMID: 25216938
  7. Nrg4 might have a protective role in the development of Non-alcoholic fatty liver disease (NAFLD). PMID: 26476959
  8. results establish Nrg4 as a brown fat-enriched endocrine factor with therapeutic potential for the treatment of obesity-associated disorders, including type 2 diabetes and nonalcoholic fatty liver disease (NAFLD). PMID: 25401691
  9. Pharmacological inhibition of PI3K/Akt signaling reversed the anti-apoptotic effects of NRG4, confirming the role of this cascade in NRG4-induced cell survival PMID: 23033483
  10. The recombinant neuregulin 4 induced the tyrosine phosphorylation of HER4. PMID: 21805036
  11. NRG4 overexpression is associated with advanced-stage prostate cancer PMID: 17545517
  12. describes a novel splice variant of the NRG4 gene, NRG4A2, which encodes a C-terminal region containing a predicted type I PDZ-binding peptide PMID: 17684490
  13. NRG4 was positive in almost all breast cancers studied. PMID: 17962208
  14. Neuregulin-4 expression is downregulated during urinary bladder cancer progression PMID: 15583696

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Subcellular Location Pro-neuregulin-4, membrane-bound isoform: Cell membrane, Single-pass type I membrane protein
Protein Families Neuregulin family
Database Links

HGNC: 29862

OMIM: 610894

KEGG: hsa:145957

STRING: 9606.ENSP00000378367

UniGene: Hs.732438

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