Human opiorphin ELISA kit

Instructions
Code CSB-EQ027423HU
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name opiorphin
Abbreviation opiorphin
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates, saliva
Detection Range 0.625 ng/mL-40 ng/mL
Sensitivity 0.156 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Others
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human opiorphin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %89
Range %82-92
1:2Average %98
Range %93-103
1:4Average %92
Range %87-96
1:8Average %92
Range %86-97
Recovery
The recovery of human opiorphin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9489-98
EDTA plasma (n=4)8681-92
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
402.561 2.669 2.615 2.491
202.205 2.114 2.160 2.036
101.457 1.354 1.406 1.282
50.742 0.720 0.731 0.607
2.50.425 0.405 0.415 0.291
1.250.275 0.283 0.279 0.155
0.6250.187 0.181 0.184 0.060
00.123 0.124 0.124
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Q&A and Customer Reviews

 Q&A
Q:

Regarding your ELISA kit CSB-EQ027423HU, could you please advise on the following:
1. Is the standard natural material or recombinant? And if recombinant, what kind of cell or living material is it used for expression?
2. Does the standard include additive agents such as antiseptic or preservative? If included, what is the composition of said additive agent(s)?
3. Material name and concentration of Stop Solution.
4. Do these kits include hazardous substance such as azide, thimerosal, 2-ME, etc?

A:
Thanks for your inquiry!
CSB-EQ027423HU
Standard is synthetic peptide.
Preservative is proclin 300(1:3000);
Stop solution is 4N sulfuric acid.
This kit doesn't contain azide, thimerosal, 2-ME, etc.
 Customer Reviews

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