Human oxidized low density lipoprotein antibody,OLAb ELISA Kit

Code CSB-E08797h
Size 96T,5×96T,10×96T How to order?
Trial Size 24T ELISA kits trial application
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Product Details

Description

This Human ox-LDL Ab ELISA Kit was designed for the quantitative measurement of Human ox-LDL Ab protein in serum, plasma. It is a Sandwich ELISA kit, its detection range is 47 μU/mL-3000 μU/mL and the sensitivity is 11.75 μU/mL.

Target Name oxidized lowdensity lipoprotein antibody,OLAb
Abbreviation ox-LDL Ab
Species Homo sapiens (Human)
Sample Types serum, plasma
Detection Range 47 μU/mL-3000 μU/mL
Sensitivity 11.75 μU/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Others
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human OLAb in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1000 Average % 90  
Range % 87-98  
1:2000 Average % 93  
Range % 91-99  
1:4000 Average % 95  
Range % 90-102  
1:8000 Average % 92  
Range % 86-98  
Recovery
The recovery of human OLAb spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 95 86-98  
EDTA plasma (n=4) 96 91-100  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
μU/ml OD1 OD2 Average Corrected  
3000 2.532 2.517 2.525 2.401  
1500 2.233 2.259 2.246 2.122  
750 1.697 1.644 1.671 1.547  
375 1.051 1.049 1.050 0.926  
187.5 0.651 0.615 0.633 0.509  
94 0.385 0.392 0.389 0.265  
47 0.248 0.251 0.250 0.126  
0 0.122 0.125 0.124    
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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