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- A 96-well Assay plate --The 96-well plate has been pre-coated with PTH 1-34.
- Negative Control (1 x 800 μl) --Eliminate false positive
- Positive Control (1 x 800 μl) --Used to evaluate the validity, stability, and comparability of experimental results.
- Sample Diluent (2x 20 ml) --Dilute the sample to an appropriate concentration.
- HRP-conjugated Immunoglobulin (1 x 10ml)--Bind to the PTH 1-34 antibody and HRP catalyzes the TMB to elicit a chromogenic reaction.
- HRP-conjugate Diluent (2x 20 ml) --(1 x 20ml)
- Wash Buffer (25x concentrate) (1 x 20 ml) --Wash away unbound or free substances.
- Substrate A (1 x 5 ml) --Mix with substrate B and interact with HRP, eliciting a Chromogenic reaction.
- TMB Substrate (1 x 10ml) --Interact with HRP, eliciting a Chromogenic reaction.
- Stop Solution (1 x 10 ml) --Stop the color reaction. The solution color immediately turns from blue to yellow.
- Four Adhesive Strips (For 96 wells)--Cover the microplate when incubating.
- An Instruction manual
