Human parathyroid hormone 1-34(PTH 1-34)antibody ELISA kit

Code CSB-EQ027274HU
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details

Target Name
parathyroid hormone 1-34(PTH 1-34)antibody
PTH 1-34 Ab
Homo sapiens (Human)
Sample Types
serum, plasma
Detection Range
Request Information
Request Information
Assay Time
Sample Volume
Detection Wavelength
450 nm
Research Area
Assay Principle
Intra-assay Precision (Precision within an assay): CV%<15%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<15%      
Three samples of known concentration were tested in twenty assays to assess.    
Typical Data
Test parameter specification test result  
Positive control >0.8 1.895  
Negative control <0.2 0.135  
Positive rate 10,Positive 100%  
Negative rate 10,Negative 100%  
Materials provided
  • A 96-well Assay plate --The 96-well plate has been pre-coated with PTH 1-34.
  • Negative Control (1 x 800 μl) --Eliminate false positive
  • Positive Control (1 x 800 μl) --Used to evaluate the validity, stability, and comparability of experimental results.
  • Sample Diluent (2x 20 ml) --Dilute the sample to an appropriate concentration.
  • HRP-conjugated Immunoglobulin (1 x 10ml)--Bind to the PTH 1-34 antibody and HRP catalyzes the TMB to elicit a chromogenic reaction.
  • HRP-conjugate Diluent (2x 20 ml) --(1 x 20ml)
  • Wash Buffer (25x concentrate) (1 x 20 ml) --Wash away unbound or free substances.
  • Substrate A (1 x 5 ml) --Mix with substrate B and interact with HRP, eliciting a Chromogenic reaction.
  • TMB Substrate (1 x 10ml) --Interact with HRP, eliciting a Chromogenic reaction.
  • Stop Solution (1 x 10 ml) --Stop the color reaction. The solution color immediately turns from blue to yellow.
  • Four Adhesive Strips (For 96 wells)--Cover the microplate when incubating.
  • An Instruction manual
Materials not provided
  • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
  • An incubator that can provide stable incubation conditions up to 37°C±5°
  • Centrifuge
  • Vortex
  • Squirt bottle, manifold dispenser, or automated microplate washer
  • Absorbent paper for blotting the microtiter plate
  • 50-300ul multi-channel micropipette
  • Pipette tips
  • Single-channel micropipette with different ranges
  • 100ml and 500ml graduated cylinders
  • Deionized or distilled water
  • Timer
  • Test tubes for dilution
and FAQs
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx

The human parathyroid hormone 1-34 (PTH 1-34) antibody ELISA kit allows for the qualitative identification of PTH 1-34 antibodies in human serum and plasma. It employs the qualitative enzyme immunoassay technique. The microtiter plate has been pre-coated with the PTH 1-34. Samples or standards are pipetted into the wells with anti-human immunoglobulin conjugated HRP. Following a wash to remove any unbound reagent, the TMB substrate solution is added to the wells and color develops in proportion to the amount of human PTH 1-34 antibody bound in the initial step. The color development is stopped and the intensity of the color is measured by a microplate reader at 450 nm. The PTH 1-34 antibody titer in the samples is determined by referring to the negative control. It indicates the presence of PTH 1-34 antibody if the O.D. (optical density) of PTH 1-34 antibody is greater than or equal to the cutoff value(2.1× Average O.D. value of negative control). There is no PTH 1-34 antibody present in the sample if the O.D. is less than the cutoff value.

This assay has excellent specificity for the detection of PTH 1-34 antibodies. And it also has been validated with precision less than 15% and lot-to-lot consistency. Get more details from the product instructions.

PTH 1-34 is a type of parathyroid hormone approved for the treatment of osteoporosis in postmenopausal women and in men who are at high risk of breaking a bone. It contributes to rebuilding bone and significantly increasing bone mineral density.


Customer Reviews and Q&A

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I am working with a PTH compound that is smaller than 1-34 so I was wondering if you could provide the immunogen that was used to generate the detection ab? Is the epitope for the detection antibody in the 7-33 range?

Thanks for your inquiry!
CSB-EQ027274HU detect the PTH 1-34 antibody.
According to the description from this customer, the user needs kit for detecting antigen (<1-34 AA), the recognition area is 7-33.
So sorry that this kit cannot match the customer's request. We only have this kit for intact PTH.
Appreciated to your kind understanding.
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