Code | CSB-E16444h |
Size | 96T,5×96T,10×96T |
Price | Request a Quote or Start an on-line Chat |
Trial Size |
24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now |
Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
Inter-assay Precision (Precision between assays): CV%<10% | ||||||
Three samples of known concentration were tested in twenty assays to assess. | ||||||
To assess the linearity of the assay, samples were spiked with high concentrations of human POSTN in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
Sample | Serum(n=4) | |||||
1:5 | Average % | 93 | ||||
Range % | 90-95 | |||||
1:10 | Average % | 103 | ||||
Range % | 99-107 | |||||
1:20 | Average % | 94 | ||||
Range % | 89-97 | |||||
1:40 | Average % | 98 | ||||
Range % | 92-103 |
The recovery of human POSTN spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
Sample Type | Average % Recovery | Range | ||||
Serum (n=5) | 94 | 89-97 | ||||
EDTA plasma (n=4) | 94 | 91-97 | ||||
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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The human Periostin (POSTN) ELISA Kit is engineered for accurate measurement of human POSTN levels from samples including serum, plasma, or tissue homogenates. It uses the Sandwich-ELISA mechanism in combination with the enzyme-substrate chromogenic reaction to measure the POSTN content in the sample. The color intensity is positively correlated with POSTN content in the sample. The POSTN concentration can be calculated according to the standard curve. This kit is tested with high sensitivity, strong specificity, good linearity, high precision and recovery, as well as lot-to-lot consistency.
POSTN, also called OSF2, is a secreted extracellular matrix (ECM) protein expressed in fibroblasts during bone and teeth development. It plays a dynamic role in mediating the communication between cells and the surrounding microenvironment rather than directly participating in the formation of the ECM. POSTN is also involved in bone density and intervertebral disc (IVD) homeostasis. It also mediates inflammation and reconstruction during bronchial asthma, as well as promotes bone development, reconstruction, repair, and strength. Expression of POSTN has been also associated with the progression and drug resistance of different tumors.
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