Human peroxiredoxin 2 (PRDX2) ELISA kit

Instructions
Code CSB-EL018654HU
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name peroxiredoxin 2 (PRDX2)
Abbreviation PRDX2
Uniprot No. P32119
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 47 pg/mL-3000 pg/mL
Sensitivity 11.75 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cell Biology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human PRDX2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %85
Range %81-95
1:2Average %97
Range %91-105
1:4Average %96
Range %92-108
1:8Average %93
Range %86-102
Recovery
The recovery of human PRDX2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9289-98
EDTA plasma (n=4)9792-100
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
30002.3812.4192.400 2.253
15001.9351.9521.944 1.797
7501.4271.4031.415 1.268
3751.0681.0591.064 0.917
187.50.6270.650.639 0.492
940.4120.4230.418 0.271
470.2950.280.288 0.141
00.1490.1450.147  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Background

Function
(From Uniprot)
Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively. Plays a role in cell protection against oxidative stress by detoxifying peroxides and as sensor of hydrogen peroxide-mediated signaling events. Might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H(2)O(2).
Subcellular Location Cytoplasm
Protein Families Peroxiredoxin family, AhpC/Prx1 subfamily
Database Links

HGNC: 9353

OMIM: 600538

KEGG: hsa:7001

STRING: 9606.ENSP00000301522

UniGene: Hs.432121

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