Human proteasome (prosome, macropain) subunit, beta type, 2 (PSMB2) ELISA kit

Code CSB-E17836h
Size 96T,5×96T,10×96T
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Product Details

Target Name
proteasome (prosome, macropain) subunit, beta type, 2
Alternative Names
HC7 I ELISA Kit; Macropain subunit C7 I ELISA Kit; Macropain subunit C7-I ELISA Kit; Multicatalytic endopeptidase complex subunit C7 1 ELISA Kit; Multicatalytic endopeptidase complex subunit C7 I ELISA Kit; Multicatalytic endopeptidase complex subunit C7-I ELISA Kit; Proteasome (prosome, macropain) subunit beta type 2 ELISA Kit; Proteasome beta 2 subunit ELISA Kit; Proteasome component C7 I ELISA Kit; Proteasome component C7-I ELISA Kit; Proteasome subunit beta type-2 ELISA Kit; PSB2_HUMAN ELISA Kit; Psmb2 ELISA Kit
Abbreviation
PSMB2
Uniprot No.
Species
Homo sapiens (Human)
Sample Types
serum, plasma, tissue homogenates, cell lysates
Detection Range
25 pg/mL-1600 pg/mL
Sensitivity
6.2 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Cell Biology
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human PSMB2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %104
Range %99-108
1:2Average %86
Range %83-89
1:4Average %94
Range %89-98
1:8Average %93
Range %88-97
Recovery
The recovery of human PSMB2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9490-98
EDTA plasma (n=4)8882-94
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
16002.750 2.852 2.801 2.656
8002.048 2.147 2.098 1.953
4001.285 1.383 1.334 1.189
2000.763 0.785 0.774 0.629
1000.416 0.424 0.420 0.275
500.251 0.262 0.257 0.112
250.204 0.210 0.207 0.062
00.144 0.145 0.145
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Human PSMB2 ELISA Kit was designed for the quantitative measurement of Human PSMB2 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 25 pg/mL-1600 pg/mL and the sensitivity is 6.2 pg/mL.

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Target Background

Function
(From Uniprot)
Non-catalytic component of the 20S core proteasome complex involved in the proteolytic degradation of most intracellular proteins. This complex plays numerous essential roles within the cell by associating with different regulatory particles. Associated with two 19S regulatory particles, forms the 26S proteasome and thus participates in the ATP-dependent degradation of ubiquitinated proteins. The 26S proteasome plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins that could impair cellular functions, and by removing proteins whose functions are no longer required. Associated with the PA200 or PA28, the 20S proteasome mediates ubiquitin-independent protein degradation. This type of proteolysis is required in several pathways including spermatogenesis (20S-PA200 complex) or generation of a subset of MHC class I-presented antigenic peptides (20S-PA28 complex).
Gene References into Functions
  1. Results showed that the overexpression of beta2 subunit decreased homologous recombination in human cells without altering the cell proteasome activity and the Rad51p level. PMID: 21660142
  2. This subunit propeptides influence cooperative proteasome assembly. PMID: 12456675
Subcellular Location
Cytoplasm. Nucleus.
Protein Families
Peptidase T1B family
Database Links

HGNC: 9539

OMIM: 602175

KEGG: hsa:5690

STRING: 9606.ENSP00000362334

UniGene: Hs.471441

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