Human selenium binding protein 1(SELENBP1) ELISA Kit

Product Details

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Target Background

Product Details

Target Name

selenium binding protein 1

Alternative Names

56 kDa selenium binding protein ELISA Kit; 56 kDa selenium-binding protein ELISA Kit; hSBP ELISA Kit; LPSB ELISA Kit; SBP ELISA Kit; SBP1 ELISA Kit; SBP1_HUMAN ELISA Kit; SBP56 ELISA Kit; Selenbp1 ELISA Kit; Selenbp2 ELISA Kit; Selenium Binding Protein 1 ELISA Kit; Selenium binding protein 2 ELISA Kit; Selenium binding protein1 ELISA Kit; Selenium-binding protein 1 ELISA Kit; SELNBP1 ELISA Kit; SP56 ELISA Kit

Abbreviation

SELENBP1

Uniprot No.

Q13228

Species

Homo sapiens (Human)

Sample Types

serum, plasma, tissue homogenates

Detection Range

125 pg/mL-8000 pg/mL

Sensitivity

31.25 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Cancer

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human SELENBP1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	84
	Range %	80-88
1:2	Average %	97
	Range %	91-105
1:4	Average %	97
	Range %	92-104
1:8	Average %	93
	Range %	86-98

Recovery

The recovery of human SELENBP1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-98
EDTA plasma (n=4)	94	90-100

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
8000	2.520	2.604	2.562	2.367
4000	2.203	2.214	2.209	2.014
2000	1.888	1.899	1.894	1.699
1000	1.445	1.496	1.471	1.276
500	1.005	1.067	1.036	0.841
250	0.673	0.681	0.677	0.482
125	0.378	0.384	0.381	0.186
0	0.194	0.196	0.195

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx

Description

This Human SELENBP1 ELISA Kit was designed for the quantitative measurement of Human SELENBP1 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 125 pg/mL-8000 pg/mL and the sensitivity is 31.25 pg/mL .

Target Background

Function
(From Uniprot)

Catalyzes the oxidation of methanethiol, an organosulfur compound known to be produced in substantial amounts by gut bacteria. Selenium-binding protein which may be involved in the sensing of reactive xenobiotics in the cytoplasm. May be involved in intra-Golgi protein transport.

Gene References into Functions

High anti-SBP1 is associated with infertile women with premature ovarian failure. PMID: 27965399
Downregulation of selenium-binding protein 1 is associated with lung squamous cell carcinoma. PMID: 26956891
cysteine 57 is a critical determinant of SBP1 function and may play a significant role in mitochondrial function. PMID: 26593911
Data indicate that loss of SBP1 may play an independent contributing role in prostate cancer progression and its levels might be useful in distinguishing indolent from aggressive disease. PMID: 25993660
elevated transcript expression is widespread throughout the prefrontal cortex in schizophrenia PMID: 26241353
These results have revealed a novel mechanism that SELENBP1-mediated cancer inhibition is through altering lipid/glucose metabolic signaling pathways. PMID: 25974208
Decreased selenium-binding protein 1 mRNA expression is associated with renal cell carcinoma. PMID: 25227434
hSP56 plays an important role in regulating HIF-1alpha, which may be one of mechanisms of hSP56 expression in suppressing the malignant characteristics of prostate cancer cells. PMID: 24874852
the downregulated SELENBP1 expression was reactivated by inducing differentiation. Therefore, SELENBP1 is a potential pharmacological target for individualized CRC treatment. PMID: 24737289
Decreased SELENBP1 expression is an early event in lung squamous cell cancer tumorigenesis. PMID: 23977169
The expression level of SELENBP1 could be an important marker for predicting survival and effectiveness of selenium supplementation in breast cancer. PMID: 23704933
These results suggest that SBP1 plays a significant role in gastric cancer cell proliferation and modulates its response to anticancer drugs PMID: 23483240
SP1 is present in reduced levels in several cancer types as compared with normal tissues, and lower levels are associated with poor clinical prognosis. PMID: 20530237
Glutathione peroxidase (GPx)-1 enzyme activity is inversely correlated with selenium binding protein (SBP)-1 levels in prostate tissue, based on the Gleason score. PMID: 22072582
The suppression of Selenium-binding protein 1 may be a late event in gastric carcinogenesis. PMID: 20480265
The 3-year survival rate of gastric cancer patients with high expression of selenium-binding protein 1 was significantly higher than that of patients with low expression PMID: 21497372
down-regulation of SBP1 may play a key role in the tumorigenic process of human gastric carcinoma PMID: 20354826
Decreased expression of selenium-binding protein 1 in uterine leiomyoma may indicate a role of the protein in tumorigenesis. PMID: 21143902
A reduced SELENBP1 expression by immunohistochemistry in liver tissues of patients with hepatocarcinoma (HCC), is reported. PMID: 21338716
evidence for recurrence of decreased copy-number of the SELENBP1 locus in three unrelated schizophrenia patients' cohorts but not in controls, raising the possibility of functional involvement of these mutations in the etiology of the disease PMID: 20615253
SELENBP1 expression may be an important predictor of response to chemoprevention or chemosensitization with certain forms of selenium in esophageal tissues. PMID: 20332323
SBP1 has tumor suppressor functions that are inhibited in colorectal cancer through epigenetic silencing PMID: 19924303
loss of selenium-binding protein 1 associated with increasing epithelial proliferation indicates selenium-binding protein 1 is involved in tumorigenesis of ovarian and micropapillary serous borderline tumor and low-grade serous carcinoma. PMID: 19896693
SBP may be involved in the initial sequential events in rapid cell outgrowth, such as determining direction of cell outgrowth and recruitment of actin monomer. PMID: 15108003
changes of SELENBP1 expression in malignant ovarian cancer are an indicator of aberration of selenium/androgen pathways and may reveal prognostic information of ovarian cancer PMID: 16380993
Suppression of SELENBP1 is a frequent and late event in colorectal carcinogenesis, and may contribute to the rapid progression of colorectal carcinoma. PMID: 16645984
Elevated SELENBP1 is a possibly consistent feature in the schizophrenic brain and this could underlie some commonalities of psychosis across the boundaries of diagnoses. PMID: 18163446
Associated with differentiation of the normal colonic epithelia and may be a positive prognostic factor for survival in stage III colorectal carcinoma PMID: 18435490
VDU1 was identified as a protein partner of hSP56. PMID: 19118533
The genetic variation in SELENBP1 may influence risk for the disorder, while this significance did not remain when other inheritance models were considered. PMID: 19596560

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Subcellular Location

Nucleus. Cytoplasm, cytosol. Membrane; Peripheral membrane protein.

Protein Families

Selenium-binding protein family

Tissue Specificity

Widely expressed. Highly expressed in liver, lung, colon, prostate, kidney and pancreas. In brain, present both in neurons and glia (at protein level). Down-regulated in lung adenocarcinoma, colorectal carcinoma and ovarian cancer. Two-fold up-regulated i

Database Links

HGNC: 10719

OMIM: 604188

KEGG: hsa:8991

STRING: 9606.ENSP00000357861

UniGene: Hs.632460

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Code	CSB-E13947h
Size	96T,5×96T,10×96T
Price	Request a Quote or Start an on-line Chat
Trial Size	24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.