Human syndecan 4 (SDC4) ELISA kit

Code CSB-EL020891HU
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details

Target Name
syndecan 4
Abbreviation
SDC4
Uniprot No.
Species
Homo sapiens (Human)
Sample Types
serum, plasma, cell lysates, tissue homogenates
Detection Range
0.625 ng/mL-40 ng/mL
Sensitivity
0.156 ng/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Signal Transduction
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human SDC4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %90
Range %85-95
1:2Average %92
Range %90-96
1:4Average %93
Range %85-100
1:8Average %94
Range %86-98
Recovery
The recovery of human SDC4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9591-99
EDTA plasma (n=4)9488-100
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
402.859 2.783 2.821 2.647
202.037 2.005 2.021 1.847
101.245 1.304 1.275 1.101
50.765 0.782 0.774 0.600
2.50.501 0.511 0.506 0.332
1.250.349 0.353 0.351 0.177
0.6250.261 0.257 0.259 0.085
00.175 0.172 0.174
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Human SDC4 ELISA Kit was designed for the quantitative measurement of Human SDC4 protein in serum, plasma, cell lysates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.625 ng/mL-40 ng/mL and the sensitivity is 0.156 ng/mL.

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Target Background

Function
(From Uniprot)
Cell surface proteoglycan that bears heparan sulfate. Regulates exosome biogenesis in concert with SDCBP and PDCD6IP.
Subcellular Location
[Isoform 1]: Membrane; Single-pass type I membrane protein. Secreted.; [Isoform 2]: Secreted.
Protein Families
Syndecan proteoglycan family
Tissue Specificity
Expressed in epithelial and fibroblastic cells.
Database Links

HGNC: 10661

OMIM: 600017

KEGG: hsa:6385

STRING: 9606.ENSP00000361818

UniGene: Hs.632267

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