Human syndecan 4 (SDC4) ELISA kit

Instructions
Code CSB-EL020891HU
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
Have Questions? Leave a Message or Start an on-line Chat

Product Details

Target Name syndecan 4
Abbreviation SDC4
Uniprot No. P31431
Species Homo sapiens (Human)
Sample Types serum, plasma, cell lysates, tissue homogenates
Detection Range 0.625 ng/mL-40 ng/mL
Sensitivity 0.156 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human SDC4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %90
Range %85-95
1:2Average %92
Range %90-96
1:4Average %93
Range %85-100
1:8Average %94
Range %86-98
Recovery
The recovery of human SDC4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9591-99
EDTA plasma (n=4)9488-100
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
402.859 2.783 2.821 2.647
202.037 2.005 2.021 1.847
101.245 1.304 1.275 1.101
50.765 0.782 0.774 0.600
2.50.501 0.511 0.506 0.332
1.250.349 0.353 0.351 0.177
0.6250.261 0.257 0.259 0.085
00.175 0.172 0.174
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 7-14 working days

Target Data

Function Cell surface proteoglycan that bears heparan sulfate. Regulates exosome biogenesis in concert with SDCBP and PDCD6IP
Subcellular Location Isoform 1: Membrane, Single-pass type I membrane protein, Secreted
Protein Families Syndecan proteoglycan family
Tissue Specificity Expressed in epithelial and fibroblastic cells.
Database Links

HGNC: 10661

OMIM: 600017

KEGG: hsa:6385

STRING: 9606.ENSP00000361818

UniGene: Hs.632267

Most popular with customers

Newsletters

Get all the latest information on Events, Sales and Offers. Sign up for newsletter today.

© 2007-2020 CUSABIO TECHNOLOGY LLC All rights reserved. 鄂ICP备15011166号-1