Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
To assess the linearity of the assay, samples were spiked with high concentrations of human TE in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
The recovery of human TE spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Average % Recovery
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
We are interesting in knowing the composition and the origin of the standard as well as capture and biotin antibody in this kit.
Thanks for your inquiry. Coated antibody: Mouse monoclonal antibody Detect antibody: Goat polyclonal antibody Standard: It is prepared with a special method and sorry we cannot provide the detailed information. Hope you could understand.