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Human telomerase,TE ELISA Kit

Citations (5) Protocol
Code CSB-E08021h
Size 96T,5×96T,10×96T How to order?
Trial Size 24T ELISA kits trial application
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Product Details

Description

This Human telomerase (TE) ELISA Kit was designed for the quantitative measurement of Human telomerase (TE) protein in serum, cell culture supernates, urine, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.312 ng/mL-20 ng/mL and the sensitivity is 0.078 ng/mL.
Target Name telomerase,TE
Alternative Names N/A
Abbreviation TE
Species Homo sapiens (Human)
Sample Types serum, cell culture supernates, urine, tissue homogenates
Detection Range 0.312 ng/mL-20 ng/mL
Sensitivity 0.078 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Epigenetics and Nuclear Signaling
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human TE in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
　SampleSerum(n=4)
1:1Average %88
Range %80-96
1:2Average %99
Range %91-105
1:4Average %94
Range %92-98
1:8Average %89
Range %86-100
Recovery
The recovery of human TE spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 8980-98
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
202.630 2.501 2.566 2.464
102.161 2.062 2.112 2.010
51.602 1.541 1.572 1.470
2.50.931 0.898 0.915 0.813
1.250.532 0.501 0.517 0.415
0.6250.322 0.314 0.318 0.216
0.3120.241 0.227 0.234 0.132
00.105 0.099 0.102 　
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
Troubleshooting
and FAQs		 ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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■ Q&A
Q:

We are interesting in knowing the composition and the origin of the standard as well as capture and biotin antibody in this kit.

A:
Thanks for your inquiry.
Coated antibody: Mouse monoclonal antibody
Detect antibody: Goat polyclonal antibody
Standard: It is prepared with a special method and sorry we cannot provide the detailed information. Hope you could understand.
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