Human telomerase,TE ELISA Kit

Code CSB-E08021h
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details

Target Name
telomerase,TE
Alternative Names
N/A
Abbreviation
TE
Species
Homo sapiens (Human)
Sample Types
serum, cell culture supernates, urine, tissue homogenates
Detection Range
0.312 ng/mL-20 ng/mL
Sensitivity
0.078 ng/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Epigenetics and Nuclear Signaling
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human TE in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %88
Range %80-96
1:2Average %99
Range %91-105
1:4Average %94
Range %92-98
1:8Average %89
Range %86-100
Recovery
The recovery of human TE spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 8980-98
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
202.630 2.501 2.566 2.464
102.161 2.062 2.112 2.010
51.602 1.541 1.572 1.470
2.50.931 0.898 0.915 0.813
1.250.532 0.501 0.517 0.415
0.6250.322 0.314 0.318 0.216
0.3120.241 0.227 0.234 0.132
00.105 0.099 0.102  
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Human telomerase (TE) ELISA Kit was designed for the quantitative measurement of Human telomerase (TE) protein in serum, cell culture supernates, urine, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.312 ng/mL-20 ng/mL and the sensitivity is 0.078 ng/mL.

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Customer Reviews and Q&A

 Customer Reviews
Average Rating:
5.0 - 1 reviews

Submit a Review here

Sample type: Serum

Sample species: Human

Sample dilution: No dilution

Review: I used CSB-E08021h to measure the telomerase content in the lytic fluid of cancer cells, diluted twice, and OD was about 0.3. The scale was normal and the telomerase quantification was consistent with the literature reports. Easy to use, if possible, make the box smaller, better preservation.

By Anonymous

 Q&A
Q:

We are interesting in knowing the composition and the origin of the standard as well as capture and biotin antibody in this kit.

A:
Thanks for your inquiry.
Coated antibody: Mouse monoclonal antibody
Detect antibody: Goat polyclonal antibody
Standard: It is prepared with a special method and sorry we cannot provide the detailed information. Hope you could understand.
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