Code | CSB-E11885h |
Size | 96T,5×96T,10×96T |
Price | Request a Quote or Start an on-line Chat |
Trial Size |
24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now |
Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
Inter-assay Precision (Precision between assays): CV%<10% | ||||||
Three samples of known concentration were tested in twenty assays to assess. | ||||||
To assess the linearity of the assay, samples were spiked with high concentrations of human VEGFR-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
Sample | Serum(n=4) | |||||
1:1 | Average % | 86 | ||||
Range % | 81-93 | |||||
1:2 | Average % | 99 | ||||
Range % | 94-104 | |||||
1:4 | Average % | 92 | ||||
Range % | 88-97 | |||||
1:8 | Average % | 85 | ||||
Range % | 81-90 |
The recovery of human VEGFR-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
Sample Type | Average % Recovery | Range | ||||
Serum (n=5) | 90 | 85-96 | ||||
EDTA plasma (n=4) | 96 | 92-100 | ||||
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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The human VEGFR-1 ELISA Kit is used to quantitatively measure human VEGFR-1 concentrations in serum, plasma, or tissue homogenates. It performs well in important characteristics, including sensitivity, specificity, precision, recovery, linearity, and lot-to-lot consistency. This assay is based on the sandwich ELISA mechanism and enzyme-substrate chromogenic reaction. The solution color develops proportionally to the amount of VEGFR-1 in the sample. And the intensity of the color can be measured at 450 nm via a microplate reader.
VEGFR-1, also called Flt-1, is a high-affinity tyrosine kinase receptor for VEGFs, including VEGF-A, VEGF-B, and PIGF. Distinct from VEGFR-2, which participates both in physiological and pathological angiogenesis, VEGFR-1 in the adult is necessary only for pathological angiogenesis. VEGFR-1-VEGF plays an essential role in tumor-related angiogenesis, tissue infiltration, and metastasis formation. Furthermore, VEGFR-1 activation contributes to the recruitment of tumor-associated macrophages (TAMs), cancer immune escape, and stimulation of migration and extracellular matrix (ECM) invasion.
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Could you please provide what the immunogen sequence that the abs in the kit were generated against?