Human α-melanocyte stimulating hormone(α-MSH) ELISA Kit

Human α-melanocyte stimulating hormone(α-MSH) ELISA Kit

Product Details

Citations

Customer Reviews and Q&A

Product Details

Citations

Related Products

Customer Reviews and Q&A

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Human α-melanocyte stimulating hormone(α-MSH) ELISA Kit

Citations (5) Protocol MSDS

Code	CSB-E09658h
Size	96T,5×96T,10×96T
Price	Request a Quote
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Product Details
Citations (5)
Related Products
Customer Reviews and Q&A

Target Name

α-melanocyte stimulating hormone(α-MSH)

Abbreviation

α-MSH

Species

Homo sapiens (Human)

Sample Types

serum, plasma, cell culture supernates, tissue homogenates

Detection Range

0.156 ng/mL-10 ng/mL

Sensitivity

0.039 ng/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Biochemicals

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human α-MSH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	95
	Range %	92-104
1:2	Average %	95
	Range %	91-104
1:4	Average %	89
	Range %	85-93
1:8	Average %	94
	Range %	86-98

Recovery

The recovery of human α-MSH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	89-95
EDTA plasma (n=4)	94	89-97

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
10	2.081	2.181	2.131	2.000
5	1.256	1.198	1.227	1.096
2.5	0.655	0.635	0.645	0.514
1.25	0.406	0.416	0.411	0.280
0.625	0.260	0.270	0.265	0.134
0.312	0.220	0.219	0.220	0.089
0.156	0.187	0.182	0.185	0.054
0	0.132	0.130	0.131

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.

Description

This Human α-MSH ELISA Kit was designed for the quantitative measurement of Human α-MSH protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.156 ng/mL-10 ng/mL and the sensitivity is 0.039 ng/mL.

Serum melanin and alpha-melanocyte stimulating hormone levels in Pakistani individuals affected with oculocutaneous albinism M Shakil,Rawal Medical Journal,2023
Particulate matter promotes hyperpigmentation via AhR/MAPK signaling activation and by increasing α-MSH paracrine levels in keratinocytes Rong Xiao,Environmental Pollution,2021
Efficacy of a wound-dressing biomaterial on prevention of postinflammatory hyperpigmentation after suction blister epidermal grafting in stable vitiligo patients: a … Z Liu,Archives of Dermatological Research,2020
A ROCK inhibitor promotes keratinocyte survival and paracrine secretion, enhancing establishment of primary human melanocytes and melanocyte‐keratinocyte co‐cultures Mi J, et al,Pigment Cell & Melanoma Research,2019
Dietary Supplementation with Raspberry Seed Oil Modulates Liver Functions, Inflammatory State, and Lipid Metabolism in Rats Fotschki B. et al,J Nutr,2015

■ Customer Reviews

Average Rating:

5.0 - 1 reviews

Submit a Review here

Sample type: Cell culture supernatant

Sample species: Human

Sample dilution: No dilution

Review: I used a human α-MSH kit. Objective: To investigate the effect of UVB on the secretion of α-MSH by keratinocytes and the effect of peptide samples. Experimental samples: After UVB irradiation, the cells were incubated in a cell incubator for 24h, and the cell secretory supernatant was collected. The OD value of the experimental group was 0.15-0.31. The above is the feedback after recent use, please check!

By Anonymous

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.