Monkey Interleukin 17A (IL-17A/IL-17) ELISA Kit

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Monkey Interleukin 17A (IL-17A/IL-17) ELISA Kit

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Protocol

Code	CSB-E08905Mk
Size	96T,5×96T,10×96T
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Target Name

interleukin 17A

Alternative Names

Interleukin-17A elisa kit;IL-17 elisa kit; IL-17A elisa kit

Abbreviation

IL-17A/IL-17

Uniprot No.

F6T3G5

Species

Monkey

Sample Types

serum, plasma, cell culture supernates

Detection Range

9.38 pg/mL-600 pg/mL.

Sensitivity

4.69 pg/mL

Assay Time

1-5h

Sample Loading Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of monkey IL-17A/IL-17 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:1	Average %	89
	Range %	82-92
1:2	Average %	93
	Range %	89-99
1:4	Average %	98
	Range %	91-103
1:8	Average %	104
	Range %	97-111

Recovery

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.


pg/ml	OD1	OD2	Average	Corrected
600	3.598	3.664	3.631	3.567
300	2.771	2.895	2.833	2.768
150	1.650	1.680	1.665	1.601
75	0.826	0.861	0.843	0.779
37.5	0.473	0.500	0.486	0.422
18.75	0.224	0.221	0.223	0.158
9.38	0.158	0.161	0.159	0.095
0	0.063	0.066	0.065

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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