Mouse β-hexosaminidase A,β-Hex A ELISA Kit

Instructions
Code CSB-E04818m
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name hexosaminidase A (alpha polypeptide)
Alternative Names Hexa ELISA Kit; Beta-hexosaminidase subunit alpha ELISA Kit; EC 3.2.1.52 ELISA Kit; Beta-N-acetylhexosaminidase subunit alpha ELISA Kit; Hexosaminidase subunit A ELISA Kit; N-acetyl-beta-glucosaminidase subunit alpha ELISA Kit
Abbreviation HEXA
Uniprot No. P29416
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates
Detection Range 3.12 μU/mL-200 μU/mL
Sensitivity 0.78 μU/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Neuroscience
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse β-Hex A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:10 Average % 92  
Range % 85-100  
1:20 Average % 93  
Range % 87-98  
1:40 Average % 90  
Range % 86-100  
1:80 Average % 90  
Range % 86-98  
Recovery
The recovery of mouse β-Hex A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 97 89-101  
EDTA plasma (n=4) 95 92-98  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
μU/ml OD1 OD2 Average Corrected  
200 2.542 2.517 2.530 2.386  
100 2.073 2.101 2.087 1.943  
50 1.452 1.464 1.458 1.314  
25 0.942 0.961 0.952 0.808  
12.5 0.416 0.402 0.409 0.265  
6.25 0.325 0.311 0.318 0.174  
3.12 0.247 0.237 0.242 0.098  
0 0.146 0.141 0.144    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Background

Function
(From Uniprot)
Responsible for the degradation of GM2 gangliosides, and a variety of other molecules containing terminal N-acetyl hexosamines, in the brain and other tissues.
Gene References into Functions
  1. Neu4 is not the only sialidase contributing to the metabolic bypass in Hexa(-/-) mice. PMID: 20862357
Subcellular Location Lysosome
Protein Families Glycosyl hydrolase 20 family
Tissue Specificity Ubiquitous. Most abundant in testis, adrenal, epididymis and heart. Low levels seen in the liver.
Database Links

KEGG: mmu:15211

STRING: 10090.ENSMUSP00000026262

UniGene: Mm.2284

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