Mouse β-hexosaminidase A,β-Hex A ELISA Kit

Code CSB-E04818m
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Description

This Mouse HEXA ELISA Kit was designed for the quantitative measurement of Mouse HEXA protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 3.12 μU/mL-200 μU/mL and the sensitivity is 0.78 μU/mL.

Target Name hexosaminidase A (alpha polypeptide)
Alternative Names Hexa ELISA Kit; Beta-hexosaminidase subunit alpha ELISA Kit; EC 3.2.1.52 ELISA Kit; Beta-N-acetylhexosaminidase subunit alpha ELISA Kit; Hexosaminidase subunit A ELISA Kit; N-acetyl-beta-glucosaminidase subunit alpha ELISA Kit
Abbreviation HEXA
Uniprot No. P29416
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates
Detection Range 3.12 μU/mL-200 μU/mL
Sensitivity 0.78 μU/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Neuroscience
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse β-Hex A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:10 Average % 92  
Range % 85-100  
1:20 Average % 93  
Range % 87-98  
1:40 Average % 90  
Range % 86-100  
1:80 Average % 90  
Range % 86-98  
Recovery
The recovery of mouse β-Hex A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 97 89-101  
EDTA plasma (n=4) 95 92-98  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
μU/ml OD1 OD2 Average Corrected  
200 2.542 2.517 2.530 2.386  
100 2.073 2.101 2.087 1.943  
50 1.452 1.464 1.458 1.314  
25 0.942 0.961 0.952 0.808  
12.5 0.416 0.402 0.409 0.265  
6.25 0.325 0.311 0.318 0.174  
3.12 0.247 0.237 0.242 0.098  
0 0.146 0.141 0.144    
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Background

Function
(From Uniprot)
Hydrolyzes the non-reducing end N-acetyl-D-hexosamine and/or sulfated N-acetyl-D-hexosamine of glycoconjugates, such as the oligosaccharide moieties from proteins and neutral glycolipids, or from certain mucopolysaccharides. The isozyme S is as active as the isozyme A on the anionic bis-sulfated glycans, the chondroitin-6-sulfate trisaccharide (C6S-3), and the dermatan sulfate pentasaccharide, and the sulfated glycosphingolipid SM2. The isozyme B does not hydrolyze each of these substrates, however hydrolyzes efficiently neutral oligosaccharide. Only the isozyme A is responsible for the degradation of GM2 gangliosides in the presence of GM2A.
Gene References into Functions
  1. Neu4 is not the only sialidase contributing to the metabolic bypass in Hexa(-/-) mice. PMID: 20862357
Subcellular Location Lysosome.
Protein Families Glycosyl hydrolase 20 family
Tissue Specificity Ubiquitous. Most abundant in testis, adrenal, epididymis and heart. Low levels seen in the liver.
Database Links

KEGG: mmu:15211

STRING: 10090.ENSMUSP00000026262

UniGene: Mm.2284

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