Mouse ADAM metallopeptidase with thrombospondin type 1 motif, 13 (ADAMTS13) ELISA kit

Code CSB-EL001301MO
See More Details 24T ELISA kits trial application
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. Q769J6
Lead Time 5-7 working days
Abbreviation ADAMTS13
Protein Biological Process 1 Blood Coagulation
Target Name ADAM metallopeptidase with thrombospondin type 1 motif, 13
Alias C9orf8, DKFZp434C2322, FLJ42993, MGC118899, MGC118900, TTP, VWFCP, vWF-CP, a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 13|vWF-cleaving protease|von Wil
Species Mus musculus (Mouse)
Protein Biological Process 3 Blood coagulation
Sample Types serum, plasma, tissue homogenates
Detection Range 78 pg/mL-5000 pg/mL
Sensitivity 19.5 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cancer
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details This gene encodes a member of the ADAMTS (a disintegrin and metalloproteinase with thrombospondin motif) protein family. Members of the family share several distinct protein modules, including a propeptide region, a metalloproteinase domain, a disintegrin-like domain, and a thrombospondin type 1 (TS) motif. Individual members of this family differ in the number of C-terminal TS motifs, and some have unique C-terminal domains. The enzyme encoded by this gene is the von Willebrand Factor (vWF)-cleaving protease, which is responsible for cleaving at the site of Tyr842-Met843 of the vWF molecule. A deficiency of this enzyme is associated with thrombotic thrombocytopenic purpura. Alternative splicing of this gene generates multiple transcript variants encoding different isoforms.
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse ADAMTS13 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1000Average %95
Range %89-98
1:2000Average %92
Range %88-95
1:4000Average %92
Range %85-95
1:8000Average %98
Range %92-101
Recovery
The recovery of mouse ADAMTS13 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9186-94
EDTA plasma (n=4)10398-107
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
50002.401 2.476 2.439 2.337
25001.884 1.875 1.880 1.778
12501.413 1.424 1.419 1.317
6250.777 0.798 0.788 0.686
3120.466 0.476 0.471 0.369
1560.265 0.258 0.262 0.160
780.176 0.184 0.180 0.078
00.101 0.103 0.102
Still Have Questions? Leave a Message or Start an on-line Chat
Function Cleaves the vWF multimers in plasma into smaller forms thereby controlling vWF-mediated platelet thrombus formation.
Subcellular Location Secreted
Tissue Specificity Plasma. Expression is consistently high in liver, medium in lung and spleen, low in skeletal muscle and undetectable in heart, brain, kidney and testis.
Database Links

KEGG: mmu:279028

STRING: 10090.ENSMUSP00000099955

UniGene: Mm.330084

Related Products

Adamts13 Antibodies

Adamts13 Antibodies for Mus musculus (Mouse)

Adamts13 Proteins

Adamts13 Proteins for Mus musculus (Mouse)

Most popular with customers

Newsletters

Get all the latest information on Events, Sales and Offers. Sign up for newsletter today.

Copyright © 2007-2018 www.cusabio.com CUSABIO TECHNOLOGY LLC All Rights Reserved.