Mouse Adropin(ENHO) ELISA kit

Instructions
Code CSB-EL007669MO
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name energy homeostasis associated
Alternative Names Enho ELISA Kit; Adropin ELISA Kit; Energy homeostasis-associated protein ELISA Kit
Abbreviation ENHO
Uniprot No. Q8K1D8
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates
Detection Range 0.312 pg/mL-20 pg/mL
Sensitivity 0.078 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Others
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%        
Three samples of known concentration were tested twenty times on one plate to assess.    
Inter-assay Precision (Precision between assays): CV%<10%        
Three samples of known concentration were tested in twenty assays to assess.      
               
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse ENHO in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
  Sample Serum(n=4)    
1:1 Average % 107    
Range % 85-112    
1:2 Average % 103    
Range % 95-107    
1:4 Average % 98    
Range % 89-102    
1:8 Average % 100    
Range % 92-104    
Recovery
The recovery of mouse ENHO spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
 
Sample Type Average % Recovery Range    
Serum (n=5) 103 92-106    
EDTA plasma (n=4) 99 88-103    
               
               
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
 
pg/ml OD1 OD2 Average Corrected    
6000 2.232 2.188 2.210 2.044    
3000 1.677 1.634 1.656 1.490    
1500 1.213 1.137 1.175 1.009    
750 0.871 0.844 0.858 0.692    
375 0.577 0.556 0.567 0.401    
187.5 0.413 0.425 0.419 0.253    
93.75 0.287 0.279 0.283 0.117    
0 0.167 0.165 0.166      
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 7-14 working days

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Target Background

Function
(From Uniprot)
Involved in the regulation of glucose homeostasis and lipid metabolism.
Gene References into Functions
  1. Adropin prevents atherosclerosis development by suppressing monocyte-endothelial cell adhesion and smooth muscle cell proliferation. PMID: 29701665
  2. An inverse correlation between serum adropin levels and mouse age that was attenuated by calorie restriction. PMID: 28922017
  3. our data suggest that adropin is a membrane-bound protein that interacts with the brain-specific Notch1 ligand NB3. PMID: 25074942
  4. these results suggest a role for adropin in regulating muscle substrate preference under various nutritional states. PMID: 24848071
  5. Release of adropin in the fed condition regulates fuel selection in skeletal muscle, promoting glucose oxidation over fat oxidation. The molecular mechanisms of adropin's effects involve acetylation (suggesting inhibition) of the transcriptional co-activator PGC1alpha, reducing PDK4 and CPT1B activity. Increased PGC1alpha acetylation by adropin may be mediated by inhibiting Sirtuin-1 (SIRT1), a PGC1alpha deacetylase. PMID: 24848071
  6. a potential endothelial protective role of adropin that is likely mediated via upregulation of endothelial NO synthase expression through the VEGFR2-phosphatidylinositol 3-kinase-Akt and VEGFR2-extracellular signal regulated kinase 1/2 pathways. PMID: 20837912
  7. Adropin has an endothelial protective function mediated via upregulation of eNOS expression through the VEGFR2-PI3K-Akt and VEGFR2-ERK1/2 pathways. Adropin therapy may thus be useful for limiting diseases characterized by endothelial dysfunction. PMID: 20837912
  8. Adropin regulated expression of hepatic lipogenic genes and adipose tissue peroxisome proliferator-activated receptor gamma, a major regulator of lipogenesis. PMID: 19041763
  9. Adropin is the name given to the secreted peptide encoded by human C9orf165. In mice, it is abundant in liver where it is regulated by dietary macronutrients. Adropin regulates expression of genes involved in lipogenesis and adipogenesis. PMID: 19041763

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Subcellular Location Secreted
Tissue Specificity Expressed in liver and brain. Expressed in regions of the brain involved in metabolic regulation.
Database Links

KEGG: mmu:69638

UniGene: Mm.372459

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