Mouse Alanine Aminotransferase(ALT/GPT)ELISA Kit

Instructions
Code CSB-E16539m
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name Alanine Aminotransferase(ALT/GPT)
Alternative Names Gpt ELISA kit; Gpt1Alanine aminotransferase 1 ELISA kit; ALT1 ELISA kit; EC 2.6.1.2 ELISA kit; Glutamate pyruvate transaminase 1 ELISA kit; GPT 1 ELISA kit; Glutamic--alanine transaminase 1 ELISA kit; Glutamic--pyruvic transaminase 1 ELISA kit
Abbreviation ALT/GPT
Uniprot No. Q8QZR5
Species Mus musculus (Mouse)
Sample Types serum, plasma, cell culture supernates, cell lysates
Detection Range 0.156 mU/mL-10 mU/mL
Sensitivity 0.039 mU/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Metabolism
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse ALT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 88  
Range % 83-95  
1:2 Average % 97  
Range % 92-101  
1:4 Average % 106  
Range % 98-113  
1:8 Average % 94  
Range % 87-97  
Recovery
The recovery of mouse ALT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 92 88-97  
EDTA plasma (n=4) 102 95-108  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
mU/ml OD1 OD2 Average Corrected  
10 2.645 2.566 2.606 2.421  
5 2.015 1.855 1.935 1.750  
2.5 1.194 1.155 1.175 0.990  
1.25 0.777 0.796 0.787 0.602  
0.625 0.492 0.461 0.477 0.292  
0.312 0.335 0.344 0.340 0.155  
0.156 0.278 0.288 0.283 0.098  
0 0.191 0.178 0.185    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Citations

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Target Data

Function Catalyzes the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate. Participates in cellular nitrogen metabolism and also in liver gluconeogenesis starting with precursors transported from skeletal muscles (By similarity).
Gene References into Functions
  1. Dexamethasone induces a metabolic adaptive ALT isoenzyme response in livers of C57/BL6 mice. PMID: 22609950
  2. PKCdelta activation and protein levels are elevated in an animal model of steatohepatitis, which was recapitulated in a cell model, supporting the conclusion that PKCdelta plays a role in ALT release, the ER stress signal, and cell death PMID: 20971848
  3. In experiments where mice were provided ethanol chronically in drinking water, where stress is minimal, higher levels of ALT were noted in animals in the ethanol group as compared with animals in the control group. PMID: 15652761
Subcellular Location Cytoplasm
Protein Families Class-I pyridoxal-phosphate-dependent aminotransferase family, Alanine aminotransferase subfamily
Tissue Specificity Mainly expressed in liver, intestine, colon and white adipose tissue.
Database Links

KEGG: mmu:76282

STRING: 10090.ENSMUSP00000023203

UniGene: Mm.30130

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