Mouse Alanine Aminotransferase(ALT/GPT)ELISA Kit

Code CSB-E16539m
Size 96T,5×96T,10×96T
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Product Details

Target Name
Alanine Aminotransferase(ALT/GPT)
Alternative Names
Gpt ELISA kit; Gpt1Alanine aminotransferase 1 ELISA kit; ALT1 ELISA kit; EC 2.6.1.2 ELISA kit; Glutamate pyruvate transaminase 1 ELISA kit; GPT 1 ELISA kit; Glutamic--alanine transaminase 1 ELISA kit; Glutamic--pyruvic transaminase 1 ELISA kit
Abbreviation
ALT/GPT
Uniprot No.
Species
Mus musculus (Mouse)
Sample Types
serum, plasma, cell culture supernates, cell lysates
Detection Range
0.156 mU/mL-10 mU/mL
Sensitivity
0.039 mU/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Metabolism
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse ALT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 88  
Range % 83-95  
1:2 Average % 97  
Range % 92-101  
1:4 Average % 106  
Range % 98-113  
1:8 Average % 94  
Range % 87-97  
Recovery
The recovery of mouse ALT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 92 88-97  
EDTA plasma (n=4) 102 95-108  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
mU/ml OD1 OD2 Average Corrected  
10 2.645 2.566 2.606 2.421  
5 2.015 1.855 1.935 1.750  
2.5 1.194 1.155 1.175 0.990  
1.25 0.777 0.796 0.787 0.602  
0.625 0.492 0.461 0.477 0.292  
0.312 0.335 0.344 0.340 0.155  
0.156 0.278 0.288 0.283 0.098  
0 0.191 0.178 0.185    
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Shelf Life
6 months
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Mouse ALT/GPT ELISA Kit was designed for the quantitative measurement of Mouse ALT/GPT protein in serum, plasma, cell culture supernates, cell lysates. It is a Sandwich ELISA kit, its detection range is 0.156 mU/mL-10 mU/mL and the sensitivity is 0.039 mU/mL.

Citations

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Target Background

Function
(From Uniprot)
Catalyzes the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate. Participates in cellular nitrogen metabolism and also in liver gluconeogenesis starting with precursors transported from skeletal muscles.
Gene References into Functions
  1. Dexamethasone induces a metabolic adaptive ALT isoenzyme response in livers of C57/BL6 mice. PMID: 22609950
  2. PKCdelta activation and protein levels are elevated in an animal model of steatohepatitis, which was recapitulated in a cell model, supporting the conclusion that PKCdelta plays a role in ALT release, the ER stress signal, and cell death PMID: 20971848
  3. In experiments where mice were provided ethanol chronically in drinking water, where stress is minimal, higher levels of ALT were noted in animals in the ethanol group as compared with animals in the control group. PMID: 15652761
Subcellular Location
Cytoplasm.
Protein Families
Class-I pyridoxal-phosphate-dependent aminotransferase family, Alanine aminotransferase subfamily
Tissue Specificity
Mainly expressed in liver, intestine, colon and white adipose tissue.
Database Links
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