Mouse Alanine Aminotransferase(ALT/GPT)ELISA Kit

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Mouse Alanine Aminotransferase(ALT/GPT)ELISA Kit

Name: Mouse Alanine Aminotransferase(ALT/GPT)ELISA Kit
Brand: CUSABIO
SKU: CSB-E16539m

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Code	CSB-E16539m
Size	96T,5×96T,10×96T
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Target Background

Target Name

Alanine Aminotransferase(ALT/GPT)

Alternative Names

Gpt ELISA kit; Gpt1Alanine aminotransferase 1 ELISA kit; ALT1 ELISA kit; EC 2.6.1.2 ELISA kit; Glutamate pyruvate transaminase 1 ELISA kit; GPT 1 ELISA kit; Glutamic--alanine transaminase 1 ELISA kit; Glutamic--pyruvic transaminase 1 ELISA kit

Abbreviation

ALT/GPT

Uniprot No.

Q8QZR5

Species

Mus musculus (Mouse)

Sample Types

serum, plasma, cell culture supernates, cell lysates

Detection Range

0.156 mU/mL-10 mU/mL

Sensitivity

0.039 mU/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Metabolism

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse ALT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	88
	Range %	83-95
1:2	Average %	97
	Range %	92-101
1:4	Average %	106
	Range %	98-113
1:8	Average %	94
	Range %	87-97

Recovery

The recovery of mouse ALT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	88-97
EDTA plasma (n=4)	102	95-108

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

mU/ml	OD1	OD2	Average	Corrected
10	2.645	2.566	2.606	2.421
5	2.015	1.855	1.935	1.750
2.5	1.194	1.155	1.175	0.990
1.25	0.777	0.796	0.787	0.602
0.625	0.492	0.461	0.477	0.292
0.312	0.335	0.344	0.340	0.155
0.156	0.278	0.288	0.283	0.098
0	0.191	0.178	0.185

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days

Corchorus olitorius L. ameliorates alcoholic liver disease by regulating gut-liver axis MH Do,Journal of Functional Foods,2021
Quantitative Functional Evaluation of Liver Fibrosis in Mice with Dynamic Contrast-enhanced Photoacoustic Imaging J Lv,Radiology,2021
Sodium butyrate protects against lipopolysaccharide-induced liver injury partially via the GPR43/β-arrestin-2/NF-κB network QJ Luo,Gastroenterology Report,2020
Estrogen protects against liver damage in sepsis through inhibiting oxidative stress mediated activation of pyroptosis signaling pathway Z Xu,PLoS One,2020
Hydrogen selenide, a vital metabolite of sodium selenite, uncouples the sulfilimine bond and promotes the reversal of liver fibrosis Dongrui Luan,SCIENCE CHINA Life Sciences,2020
Inhibition of Inflammatory Responses by Centella asiatica via Suppression of IRAK1-TAK1 in Mouse Macrophages YC Cho,Am J Chin Med,2020
T cells expressing a chimeric‐PD1‐Dap10‐CD3zeta receptor reduce tumor burden in multiple murine syngeneic models of solid cancer G Parriott,Immunology,2020
2,3,5,4'tetrahydroxystilbene2OβDglucoside attenuates methionine and cholinedeficient dietinduced nonalcoholic fatty liver disease Han.et al,Experimental and Therapeutic Medicine,2018
2,3,5,4'-tetrahydroxy‑stilbene-2-O-β-D-glucoside attenuates methionine and choline‑deficient diet‑induced non‑alcoholic fatty liver disease Mingnuan Han.et al,Experimental and Therapeutic Medicine,2018
NMI and IFP35 serve as proinflammatory DAMPs during cellular infection and injury Xiahou Z.et al,Nat Commun.,2017
A water-soluble nucleolin aptamer-paclitaxel conjugate for tumor-specific targeting in ovarian cancer Li F.et al,Nat Commun.,2017
The Effects of Taoren-Honghua Herb Pair on Pathological Microvessel and Angiogenesis-Associated Signaling Pathway in Mice Model of CCl4-Induced Chronic Liver Disease Xi S.et al,Evidence-Based Complementary and Alternative Medicine,2016
Is plasma soluble CD36 associated with cardiovascular risk factors in early onset coronary artery disease patients? Krzystolik A et al,Scand J Clin Lab Invest,2015
FTO-dependent function of N6-methyladenosine is involved in the hepatoprotective effects of betaine on adolescent mice Chen J. et al,J Physiol Biochem,2015

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Function (From Uniprot)	Catalyzes the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate. Participates in cellular nitrogen metabolism and also in liver gluconeogenesis starting with precursors transported from skeletal muscles (By similarity).
Gene References into Functions	Dexamethasone induces a metabolic adaptive ALT isoenzyme response in livers of C57/BL6 mice. PMID: 22609950 PKCdelta activation and protein levels are elevated in an animal model of steatohepatitis, which was recapitulated in a cell model, supporting the conclusion that PKCdelta plays a role in ALT release, the ER stress signal, and cell death PMID: 20971848 In experiments where mice were provided ethanol chronically in drinking water, where stress is minimal, higher levels of ALT were noted in animals in the ethanol group as compared with animals in the control group. PMID: 15652761
Subcellular Location	Cytoplasm
Protein Families	Class-I pyridoxal-phosphate-dependent aminotransferase family, Alanine aminotransferase subfamily
Tissue Specificity	Mainly expressed in liver, intestine, colon and white adipose tissue.
Database Links	KEGG: mmu:76282 STRING: 10090.ENSMUSP00000023203 UniGene: Mm.30130

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

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