Mouse Alpha-fetoprotein,AFP ELISA Kit

Code CSB-E08282m
Size 96T,5×96T,10×96T How to order?
Trial Size 24T ELISA kits trial application
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Product Details

Description

The mouse Alpha-fetoprotein (AFP) ELISA Kit is intended to measure mouse AFP in serum, plasma, or tissue homogenates. This assay uses the bi-antibody sandwich technique and enzyme-substrate chromogenic reaction to quantify mouse AFP concentration. The intensity of the colored product is directly proportional to the concentration of AFP present in the sample. This mouse AFP ELISA kit has high specificity and excellent sensitivity.

AFP is the most abundant serum protein before birth, and its levels fall to very low but detectable levels after birth. Healthy adults should have very low AFP levels. It is produced by the fetal yolk sac, fetal and regenerating adult liver, and fetal gastrointestinal epithelium. AFP is an oncofetal glycoprotein expressed in the yolk sac tumor and its variants, including hepatoid and endometrioid yolk sac tumors. Elevated serum AFP concentrations have been used as a clinical biomarker for the detection of liver cancer or ovarian or testicular cancers, as well as liver diseases such as cirrhosis and hepatitis.

Alternative Names AfpAlpha-fetoprotein ELISA kit; Alpha-1-fetoprotein ELISA kit; Alpha-fetoglobulin ELISA kit
Abbreviation AFP
Uniprot No. P02772
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates.
Detection Range 1.23 ng/ml-900 ng/ml.
Sensitivity 1.23 ng/ml.
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cancer
Assay Principle quantitative
Measurement Sandwich
Linearity

  

Recovery

  

ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Background

Function
(From Uniprot)
Binds estrogens, fatty acids and metals.
Gene References into Functions
  1. endogenous AFP expression in the perinatal liver is reduced in the absence of beta-catenin, suggesting that beta-catenin regulates AFP during liver development. PMID: 22544812
  2. Findings indicate that AFP expressed under experimental cryptorchid conditions plays a role as a regulatory factor in spermatogenesis and in hepatic generation. PMID: 21573244
  3. AFP mRNA and VEGF-C mRNA in peripheral blood were significantly correlated with recurrence and metastasis of hepatocellular carcinoma. PMID: 20506647
  4. A DEHP treatment-induced phenotype was identified with lack of glycogen accumula-tion and intracytoplasmic localization of beta-catenin which was associated with increased AFP gene expression. PMID: 20307648
  5. The deficits observed in parental behavior of AFP knockout females could not be explained by any changes in olfactory function, novelty recognition or anxiety PMID: 20109458
  6. Foxa1 acts as a pioneer transcription factor in de novo activation of Afp, by exploiting a lack of methylation at juxtaposed transposed elements PMID: 20348100
  7. The level of AFP mRNA in peripheral blood may indicate recurrence and/or metastasis after curative resection of hepatocellular carcinoma. PMID: 12113675
  8. AFP affects cell proliferation by binding its receptor to trigger the signal transduction pathway of cAMP-PKA and alter the expression of K- ras p21 genes PMID: 12118941
  9. A murine Nkx2.8 was isolated from the Hepal-6 cell line and showed oligonucleotide binding competitive with fetoprotein transcription factor. PMID: 12167706
  10. The mouse alpha-fetoprotein promoter is repressed in HepG2 hepatoma cells by hepatocyte nuclear factor-3 PMID: 12215259
  11. AFP has a role in female fertility, but not in male fertility or survival, in mice PMID: 12297623
  12. mouse and human alpha-fetoprotein enhancers are strikingly different PMID: 15028291
  13. AFP protects the developing female brain from the adverse effects of prenatal estrogen exposure and clarify a long-running debate on the role of this fetal protein in brain sexual differentiation PMID: 16479017
  14. 30-kDa protein is a novel isoform of AUF1 family and is the main component of the DAP-II complex that binds to the DAS sequence PMID: 16514630
  15. The sex difference in the ability to show preovulatory LH surges depends on the prenatal actions of estrogens in the male hypothalamus, is lost in Afp(-/-) females. PMID: 18202134
  16. data indicate that this enhancer region is required for alpha-fetoprotein and albumin activation early in liver development and alpha-fetoprotein reactivation during liver regeneration PMID: 19782060

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Subcellular Location Secreted.
Protein Families ALB/AFP/VDB family
Tissue Specificity Plasma.
Database Links

KEGG: mmu:11576

STRING: 10090.ENSMUSP00000041006

UniGene: Mm.358570

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