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Mouse Alpha-fetoprotein,AFP ELISA Kit

Mouse Alpha-fetoprotein,AFP ELISA Kit

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Citations (6) Protocol MSDS

Code	CSB-E08282m
Size	96T,5×96T,10×96T
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Product Details
Citations (6)
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Customer Reviews and Q&A
Target Background

Product Details

Alternative Names

AfpAlpha-fetoprotein ELISA kit; Alpha-1-fetoprotein ELISA kit; Alpha-fetoglobulin ELISA kit

Abbreviation

AFP

Uniprot No.

P02772

Species

Mus musculus (Mouse)

Sample Types

serum, plasma, tissue homogenates.

Detection Range

1.23 ng/ml-900 ng/ml.

Sensitivity

1.23 ng/ml.

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Cancer

Assay Principle

quantitative

Measurement

Sandwich

Precision

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean(ng/ml)	34.056	34.846	35.720	33.819	34.253	34.648
SD	0.035	0.038	0.042	0.036	0.042	0.053
CV(%)	4.481	4.744	5.103	4.645	5.344	6.658

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse AFP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

Sample

Serum(n=4)

1:1

Average %

Range %

82-93

1:2

Average %

Range %

94-104

1:4

Average %

Range %

96-108

1:8

Average %

Range %

80-100

Recovery

The recovery of mouse AFP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	91	83-97
EDTA plasma (n=4)	96	92-105

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
900	2.379	2.252	2.316	2.161
300	1.975	1.876	1.926	1.771
100	1.305	1.304	1.305	1.150
33.33	0.812	0.779	0.796	0.641
11.11	0.534	0.494	0.514	0.359
3.7	0.372	0.349	0.361	0.206
1.23	0.264	0.249	0.257	0.102
0	0.161	0.149	0.155

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.

Description

The mouse Alpha-fetoprotein (AFP) ELISA Kit is intended to measure mouse AFP in serum, plasma, or tissue homogenates. This assay uses the bi-antibody sandwich technique and enzyme-substrate chromogenic reaction to quantify mouse AFP concentration. The intensity of the colored product is directly proportional to the concentration of AFP present in the sample. This mouse AFP ELISA kit has high specificity and excellent sensitivity.

AFP is the most abundant serum protein before birth, and its levels fall to very low but detectable levels after birth. Healthy adults should have very low AFP levels. It is produced by the fetal yolk sac, fetal and regenerating adult liver, and fetal gastrointestinal epithelium. AFP is an oncofetal glycoprotein expressed in the yolk sac tumor and its variants, including hepatoid and endometrioid yolk sac tumors. Elevated serum AFP concentrations have been used as a clinical biomarker for the detection of liver cancer or ovarian or testicular cancers, as well as liver diseases such as cirrhosis and hepatitis.

Citations

Re-evaluation of the anticarcinogenic effect of metformin in a chemically-induced hepatocellular carcinoma model not associated with diabetes F AM El-Deeb,Egyptian Journal of Basic and Applied Sciences,2022
Possible ameliorating effect of isoquercetin on diethylnitrosamine-induced hepatocellular carcinoma in albino mice Kholoud A. Omran,Egyptian Journal of Basic and Applied Sciences,2022
Spirulina inhibits hepatocellular carcinoma through activating p53 and apoptosis and suppressing oxidative stress and angiogenesis Yomna I Mahmoud,Life Sciences,2020
Oleanolic Acid Suppressed DMBA-Induced Liver Carcinogenesis through Induction of Mitochondrial-Mediated Apoptosis and Autophagy S Hosny,Nutr Cancer,2020
Effect of protocatechuic acid-layered double hydroxide nanoparticles on diethylnitrosamine/phenobarbital-induced hepatocellular carcinoma in mice Gani SA,PLoS One,2019
Screening of biomarkers for liver adenoma in low-dose-rate gamma-ray-irradiated mice. Sugihara T.et al,Int J Radiat Biol,2018

Customer Reviews and Q&A

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Target Background

Function
(From Uniprot)

Binds estrogens, fatty acids and metals.

Gene References into Functions

endogenous AFP expression in the perinatal liver is reduced in the absence of beta-catenin, suggesting that beta-catenin regulates AFP during liver development. PMID: 22544812
Findings indicate that AFP expressed under experimental cryptorchid conditions plays a role as a regulatory factor in spermatogenesis and in hepatic generation. PMID: 21573244
AFP mRNA and VEGF-C mRNA in peripheral blood were significantly correlated with recurrence and metastasis of hepatocellular carcinoma. PMID: 20506647
A DEHP treatment-induced phenotype was identified with lack of glycogen accumula-tion and intracytoplasmic localization of beta-catenin which was associated with increased AFP gene expression. PMID: 20307648
The deficits observed in parental behavior of AFP knockout females could not be explained by any changes in olfactory function, novelty recognition or anxiety PMID: 20109458
Foxa1 acts as a pioneer transcription factor in de novo activation of Afp, by exploiting a lack of methylation at juxtaposed transposed elements PMID: 20348100
The level of AFP mRNA in peripheral blood may indicate recurrence and/or metastasis after curative resection of hepatocellular carcinoma. PMID: 12113675
AFP affects cell proliferation by binding its receptor to trigger the signal transduction pathway of cAMP-PKA and alter the expression of K- ras p21 genes PMID: 12118941
A murine Nkx2.8 was isolated from the Hepal-6 cell line and showed oligonucleotide binding competitive with fetoprotein transcription factor. PMID: 12167706
The mouse alpha-fetoprotein promoter is repressed in HepG2 hepatoma cells by hepatocyte nuclear factor-3 PMID: 12215259
AFP has a role in female fertility, but not in male fertility or survival, in mice PMID: 12297623
mouse and human alpha-fetoprotein enhancers are strikingly different PMID: 15028291
AFP protects the developing female brain from the adverse effects of prenatal estrogen exposure and clarify a long-running debate on the role of this fetal protein in brain sexual differentiation PMID: 16479017
30-kDa protein is a novel isoform of AUF1 family and is the main component of the DAP-II complex that binds to the DAS sequence PMID: 16514630
The sex difference in the ability to show preovulatory LH surges depends on the prenatal actions of estrogens in the male hypothalamus, is lost in Afp(-/-) females. PMID: 18202134
data indicate that this enhancer region is required for alpha-fetoprotein and albumin activation early in liver development and alpha-fetoprotein reactivation during liver regeneration PMID: 19782060

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Subcellular Location

Secreted.

Protein Families

ALB/AFP/VDB family

Tissue Specificity

Plasma.

Database Links

KEGG: mmu:11576

STRING: 10090.ENSMUSP00000041006

UniGene: Mm.358570

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Email: [email protected]
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Related pathways

Hippo signaling pathway Cell markers

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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Mouse Alpha-fetoprotein,AFP ELISA Kit

Product Details

Citations

Related Products

Customer Reviews and Q&A

Target Background