Mouse Aminopeptidase N(ANPEP) ELISA kit

Product Details

Citations

Related Products

Customer Reviews and Q&A

Target Background

Product Details

Alternative Names

Anpep ELISA kit; Lap-1 ELISA kit; Lap1Aminopeptidase N ELISA kit; AP-N ELISA kit; mAPN ELISA kit; EC 3.4.11.2 ELISA kit; Alanyl aminopeptidase ELISA kit; Aminopeptidase M ELISA kit; AP-M ELISA kit; Membrane protein p161 ELISA kit; Microsomal aminopeptidase ELISA kit; CD antigen CD13 ELISA kit

Abbreviation

ANPEP

Uniprot No.

P97449

Species

Mus musculus (Mouse)

Sample Types

serum, plasma, tissue homogenates

Detection Range

39 mU/mL-2500 mU/mL

Sensitivity

9.7 mU/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Cardiovascular

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse ANPEP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	88
	Range %	84-97
1:2	Average %	95
	Range %	90-99
1:4	Average %	102
	Range %	95-106
1:8	Average %	95
	Range %	89-99

Recovery

The recovery of mouse ANPEP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	88	82-96
EDTA plasma (n=4)	97	93-104

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

mU/ml	OD1	OD2	Average	Corrected
2500	2.500	2.400	2.450	2.285
1250	1.970	1.830	1.900	1.735
625	1.119	1.075	1.097	0.932
312.5	0.706	0.717	0.712	0.547
156.25	0.447	0.458	0.453	0.288
78	0.304	0.315	0.310	0.145
39	0.246	0.252	0.249	0.084
0	0.164	0.165	0.165

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx

Description

This Mouse ANPEP ELISA Kit was designed for the quantitative measurement of Mouse ANPEP protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 39 mU/mL-2500 mU/mL and the sensitivity is 9.7 mU/mL .

Target Background

Function
(From Uniprot)

Broad specificity aminopeptidase which plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases. Also involved in the processing of various peptides including peptide hormones, such as angiotensin III and IV, neuropeptides, and chemokines. May also be involved the cleavage of peptides bound to major histocompatibility complex class II molecules of antigen presenting cells. May have a role in angiogenesis and promote cholesterol crystallization. May have a role in amino acid transport by acting as binding partner of amino acid transporter SLC6A19 and regulating its activity.

Gene References into Functions

we concluded that APN plays a significant role in the regulation of several sperm functions and early embryonic development. In addition, increased APN activity could potentially lead to several adverse consequences related to male fertility. PMID: 28859152
These studies identified CD13 as a novel negative regulator of mast cell activation in vitro and in vivo. PMID: 26936360
14-3-3epsilon might directly bind to CD13, which transmits its signal in chondrocytes to induce a catabolic phenotype similar to that observed in osteoarthritis. PMID: 26208633
Alanyl aminopeptidase expression is confined to mature zymogenic chief cells and its expression is lost en route to metaplasia. PMID: 26514774
CD13 negatively regulates TLR4 signaling, thereby balancing the innate response by maintaining the inflammatory equilibrium critical to innate immune regulation. PMID: 25801433
The impact of loss of CD13 on a model of ischemic skeletal muscle injury, was investigated. PMID: 24307555
Molecular mechanisms regulating CD13-mediated adhesion. PMID: 24627994
CD13 is essential for proper trafficking of the inflammatory cells necessary to prime and sustain the reparative response, thus promoting optimal post-infarction healing. PMID: 23761403
Tyrosine phosphorylation of CD13 regulates inflammatory cell-cell adhesion and monocyte trafficking. PMID: 23997214
This indicates that Anpep plays a critical role in the proteolytic remodelling of mammary tissue during adult mammary development. PMID: 22983824
the presence of digestive protein complexes in the intestinal brush-border containing the peptidases APN and ACE2 and the neutral amino acid transporter B0AT1 PMID: 22677001
cooperation in APN expression by both cancer cells and nonmalignant stromal cells within the tumor microenvironment promotes angiogenesis, tumor growth, and metastasis PMID: 22307623
expression of CD13 in normal and pathological human tissues PMID: 21339174
Data show that the majority of chondrocyte-like cells are of bone marrow origin, whereas CD34(+)/CD13(+) myeloid precursors appear to infiltrate the plaque actively and transdifferentiate into chondrocyte-like cells in the progression of atherosclerosis. PMID: 20489139
although CD13 is highly expressed on myeloid cells and is a reliable marker of the myeloid lineage of normal and leukemic cells, it is not a critical regulator of hematopoietic development, hemostasis, or myeloid cell function PMID: 20430777
CD13 expressed in microglia-derived exosomes is active in neuropeptide degradation and could have a distinct effect on local neuropeptide activity in the brain. PMID: 16081791
Results suggest that CD26 and CD13 play a role in T cell function in tissue-specific autoimmunity in the central nervous system. PMID: 16700521
In cardiac angiogenesis endothelial CD13/APN is upregulated. It can be targeted specifically with cNGR conjugates. In the heart cNGR binds its endothelial target only in angiogenic areas. PMID: 16990557
aminopeptidase N has a role in angiogenesis PMID: 17360568

Hide All

Subcellular Location

Cell membrane; Single-pass type II membrane protein.

Protein Families

Peptidase M1 family

Tissue Specificity

Expressed in the intestinal brush border (at protein level). Highly expressed in intestinal tract and kidney, present in liver, lymph node, spleen, and brain. Found as well in monocytes, macrophages, dendritic cells, veiled cells and B-cells but not on T-

Database Links

KEGG: mmu:16790

STRING: 10090.ENSMUSP00000035943

UniGene: Mm.4487

Message

Product >> Product Name：
Product >> Code：
Name： *
Email： *
Company/Organization：
Phone：
Since emails may get flagged by spam filters occasionally, we recommend leaving your phone number as a precaution. Your information is safe with us.
Question/Comment： *
Your Region： *
I'd like to receive email newsletters from Cusabio

Yes

No
How do you know CUSABIO?

Email

Distributor

Search engine

Advertisement

Others
CAPTCHA verification：

Code	CSB-EL001827MO
Size	96T,5×96T,10×96T
Price	Request a Quote or Start an on-line Chat
Trial Size	24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.