Mouse Aminopeptidase N(ANPEP) ELISA kit

Instructions
Code CSB-EL001827MO
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name alanyl (membrane) aminopeptidase
Alternative Names Anpep ELISA kit; Lap-1 ELISA kit; Lap1Aminopeptidase N ELISA kit; AP-N ELISA kit; mAPN ELISA kit; EC 3.4.11.2 ELISA kit; Alanyl aminopeptidase ELISA kit; Aminopeptidase M ELISA kit; AP-M ELISA kit; Membrane protein p161 ELISA kit; Microsomal aminopeptidase ELISA kit; CD antigen CD13 ELISA kit
Abbreviation ANPEP
Uniprot No. P97449
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates
Detection Range 39 mU/mL-2500 mU/mL
Sensitivity 9.7 mU/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cardiovascular
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse ANPEP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %88
Range %84-97
1:2Average %95
Range %90-99
1:4Average %102
Range %95-106
1:8Average %95
Range %89-99
Recovery
The recovery of mouse ANPEP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 8882-96
EDTA plasma (n=4)9793-104
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
mU/mlOD1OD2AverageCorrected
25002.500 2.400 2.450 2.285
12501.970 1.830 1.900 1.735
6251.119 1.075 1.097 0.932
312.50.706 0.717 0.712 0.547
156.250.447 0.458 0.453 0.288
780.304 0.315 0.310 0.145
390.246 0.252 0.249 0.084
00.164 0.165 0.165  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 5-7 working days

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Target Data

Function Broad specificity aminopeptidase which plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases. Also involved in the processing of various peptides including peptide hormones, such as angiotensin III and IV, neuropeptides, and chemokines (By similarity). May also be involved the cleavage of peptides bound to major histocompatibility complex class II molecules of antigen presenting cells
Gene References into Functions
  1. we concluded that APN plays a significant role in the regulation of several sperm functions and early embryonic development. In addition, increased APN activity could potentially lead to several adverse consequences related to male fertility. PMID: 28859152
  2. These studies identified CD13 as a novel negative regulator of mast cell activation in vitro and in vivo. PMID: 26936360
  3. 14-3-3epsilon might directly bind to CD13, which transmits its signal in chondrocytes to induce a catabolic phenotype similar to that observed in osteoarthritis. PMID: 26208633
  4. Alanyl aminopeptidase expression is confined to mature zymogenic chief cells and its expression is lost en route to metaplasia. PMID: 26514774
  5. CD13 negatively regulates TLR4 signaling, thereby balancing the innate response by maintaining the inflammatory equilibrium critical to innate immune regulation. PMID: 25801433
  6. The impact of loss of CD13 on a model of ischemic skeletal muscle injury, was investigated. PMID: 24307555
  7. Molecular mechanisms regulating CD13-mediated adhesion. PMID: 24627994
  8. CD13 is essential for proper trafficking of the inflammatory cells necessary to prime and sustain the reparative response, thus promoting optimal post-infarction healing. PMID: 23761403
  9. Tyrosine phosphorylation of CD13 regulates inflammatory cell-cell adhesion and monocyte trafficking. PMID: 23997214
  10. This indicates that Anpep plays a critical role in the proteolytic remodelling of mammary tissue during adult mammary development. PMID: 22983824
  11. the presence of digestive protein complexes in the intestinal brush-border containing the peptidases APN and ACE2 and the neutral amino acid transporter B0AT1 PMID: 22677001
  12. cooperation in APN expression by both cancer cells and nonmalignant stromal cells within the tumor microenvironment promotes angiogenesis, tumor growth, and metastasis PMID: 22307623
  13. expression of CD13 in normal and pathological human tissues PMID: 21339174
  14. Data show that the majority of chondrocyte-like cells are of bone marrow origin, whereas CD34(+)/CD13(+) myeloid precursors appear to infiltrate the plaque actively and transdifferentiate into chondrocyte-like cells in the progression of atherosclerosis. PMID: 20489139
  15. although CD13 is highly expressed on myeloid cells and is a reliable marker of the myeloid lineage of normal and leukemic cells, it is not a critical regulator of hematopoietic development, hemostasis, or myeloid cell function PMID: 20430777
  16. CD13 expressed in microglia-derived exosomes is active in neuropeptide degradation and could have a distinct effect on local neuropeptide activity in the brain. PMID: 16081791
  17. Results suggest that CD26 and CD13 play a role in T cell function in tissue-specific autoimmunity in the central nervous system. PMID: 16700521
  18. In cardiac angiogenesis endothelial CD13/APN is upregulated. It can be targeted specifically with cNGR conjugates. In the heart cNGR binds its endothelial target only in angiogenic areas. PMID: 16990557
  19. aminopeptidase N has a role in angiogenesis PMID: 17360568

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Subcellular Location Cell membrane, Single-pass type II membrane protein
Protein Families Peptidase M1 family
Tissue Specificity Highly expressed in intestinal tract and kidney, present in liver, lymph node, spleen, and brain. Found as well in monocytes, macrophages, dendritic cells, veiled cells and B-cells but not on T-cells and thymocytes.
Database Links

KEGG: mmu:16790

STRING: 10090.ENSMUSP00000035943

UniGene: Mm.4487

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