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| Code | CSB-EL001715MO |
| Size | 96T,5×96T,10×96T |
| Price | Request a Quote or Start an on-line Chat |
| Trial Size |
24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now |
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse ANGPTL7 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 90 | ||||
| Range % | 86-94 | |||||
| 1:2 | Average % | 98 | ||||
| Range % | 93-103 | |||||
| 1:4 | Average % | 102 | ||||
| Range % | 95-109 | |||||
| 1:8 | Average % | 94 | ||||
| Range % | 90-98 | |||||
| The recovery of mouse ANGPTL7 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 97 | 93-101 | ||||
| EDTA plasma (n=4) | 93 | 88-98 | ||||
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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The mouse angiopoietin-related protein 7 (ANGPTL7) ELISA Kit is used to quantitatively measure mouse ANGPTL7 concentrations in serum, plasma, or tissue homogenates. It performs well in important characteristics, including sensitivity and specificity. This assay is based on the sandwich ELISA mechanism and enzyme-substrate chromogenic reaction. The solution color develops proportionally to the amount of ANGPTL7 in the sample. And the intensity of the color can be measured at 450 nm via a microplate reader.
ANGPTL7, initially discovered in the stromal layer of the cornea, contributes to coordinating the trabecular meshwork's extracellular matrix and its response to steroids. Levels of ANGPTL7 has been shown to be increased in glaucoma and its overexpression elevates the collagen expression level. While ANGPTL7 induction by glucocorticoids led to the up-regulation of important glaucoma-related proteins including fibronectin, myocilin, and MMP1. ANGPTL7 overexpression has been detected in different human cancers. It exerts roles in various cancers through its interaction with the WNT/-beta-catenin signaling pathway.
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