Mouse Apolipoprotein A-I-binding protein(APOA1BP) ELISA kit

Code CSB-EL001914MO
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Description

This Mouse APOA1BP ELISA Kit was designed for the quantitative measurement of Mouse APOA1BP protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 9.375 pg/mL-600 pg/mL and the sensitivity is 2.34 pg/mL.

Target Name apolipoprotein A-I binding protein
Alternative Names Naxe ELISA Kit; Aibp ELISA Kit; Apoa1bp ELISA Kit; NAD(P)H-hydrate epimerase ELISA Kit; EC 5.1.99.6 ELISA Kit; Apolipoprotein A-I-binding protein ELISA Kit; AI-BP ELISA Kit; NAD(P)HX epimerase ELISA Kit
Abbreviation APOA1BP
Uniprot No. Q8K4Z3
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates
Detection Range 9.375 pg/mL-600 pg/mL
Sensitivity 2.34 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cardiovascular
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse APOA1BP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1000Average %91
Range %86-95
1:2000Average %102
Range %97-107
1:4000Average %91
Range %85-97
1:8000Average %97
Range %91-103
Recovery
The recovery of mouse APOA1BP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9589-98
EDTA plasma (n=4)9790-100
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
6002.634 2.698 2.666 2.571
3002.215 2.265 2.240 2.145
1501.603 1.661 1.632 1.537
750.899 0.872 0.886 0.791
37.50.456 0.468 0.462 0.367
18.750.312 0.332 0.322 0.227
9.3750.218 0.230 0.224 0.129
00.096 0.094 0.095
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Background

Function
(From Uniprot)
Catalyzes the epimerization of the S- and R-forms of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration. This is a prerequisite for the S-specific NAD(P)H-hydrate dehydratase to allow the repair of both epimers of NAD(P)HX. Accelerates cholesterol efflux from endothelial cells to high-density lipoprotein (HDL) and thereby regulates angiogenesis.
Gene References into Functions
  1. Loss of Apoa1bp increased vascular density and facilitated the recovery of blood vessel perfusion function in a hindlimb ischemia model. PMID: 28325782
  2. The Aibp gene encodes two different proteins, both targeted to the mitochondria (mAIBP) and cytosol (cAIBP) confirming the presence of dehydratase and epimerase in the cytosol and mitochondria. PMID: 24611804
  3. AI-BP plays an important role in capacitation possibly providing a link between protein phosphorylation and cholesterol efflux. PMID: 18202122
Subcellular Location Mitochondrion. Secreted. Note=In sperm, secretion gradually increases during capacitation.
Protein Families NnrE/AIBP family
Tissue Specificity Detected in testis and sperm (at protein level). Expressed at high levels in heart, liver, kidney, and testis.
Database Links

KEGG: mmu:246703

STRING: 10090.ENSMUSP00000029708

UniGene: Mm.205996

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