Mouse Apolipoprotein B48 (apo-B48) ELISA Kit

Code CSB-E16506m
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name Apolipoprotein B48 (apo-B48)
Abbreviation apo-B48
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates
Detection Range 62.5 ng/mL-4000 ng/mL
Sensitivity 15.6 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Metabolism
Assay Principle quantitative
Measurement Competitive
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
To assess the linearity of the assay, samples were spiked with high concentrations of mouse apo-B48 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:200 Average % 96  
Range % 92-104  
1:400 Average % 93  
Range % 89-97  
1:800 Average % 87  
Range % 84-92  
1:1600 Average % 87  
Range % 82-93  
The recovery of mouse apo-B48 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 94 89-99  
EDTA plasma (n=4) 98 94-102  
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average    
4000 0.073 0.074 0.074    
2000 0.117 0.120 0.119    
1000 0.183 0.192 0.188    
500 0.283 0.275 0.279    
250 0.451 0.448 0.450    
125 0.770 0.768 0.769    
62.5 1.048 1.122 1.085    
0 2.275 2.371 2.323    
and FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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