Mouse Bone morphogenetic protein 2,BMP-2 ELISA Kit

Instructions
Code CSB-E04509m
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name bone morphogenetic protein 2
Alternative Names Bmp2 ELISA Kit; Bmp-2 ELISA Kit; Bone morphogenetic protein 2 ELISA Kit; BMP-2 ELISA Kit; Bone morphogenetic protein 2A ELISA Kit; BMP-2A ELISA Kit
Abbreviation BMP2
Uniprot No. P21274
Species Mus musculus (Mouse)
Sample Types serum, plasma, cell culture supernates, tissue homogenates
Detection Range 1.56 pg/mL-100 pg/mL
Sensitivity 0.39 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse BMP-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 97  
Range % 88-101  
1:2 Average % 101  
Range % 95-105  
1:4 Average % 90  
Range % 85-94  
1:8 Average % 99  
Range % 91-103  
Recovery
The recovery of mouse BMP-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 88 80-92  
EDTA plasma (n=4) 101 92-107  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
100 2.643 2.453 2.548 2.423  
50 2.024 1.976 2.000 1.875  
25 1.349 1.312 1.331 1.206  
12.5 0.742 0.724 0.733 0.608  
6.25 0.449 0.467 0.458 0.333  
3.12 0.307 0.300 0.304 0.179  
1.56 0.242 0.237 0.240 0.115  
0 0.126 0.124 0.125    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Background

Function
(From Uniprot)
Induces cartilage and bone formation. Stimulates the differentiation of myoblasts into osteoblasts via the EIF2AK3-EIF2A- ATF4 pathway. BMP2 activation of EIF2AK3 stimulates phosphorylation of EIF2A which leads to increased expression of ATF4 which plays a central role in osteoblast differentiation. In addition stimulates TMEM119, which upregulates the expression of ATF4.
Gene References into Functions
  1. TNAP activation in vascular smooth muscle cells (VSMCs) appears sufficient to induce calcification. TNAP activation in VSMCs stimulates expression of chondrocyte markers. PMID: 27932058
  2. widespread myocardial Bmp2 and endocardial Notch signaling drive presumptive ventricular endocardium to differentiate into valve endocardium PMID: 29853617
  3. BMP-2 can enhance HUVEC proliferation, migration and angiogenesis through P38, ERK and Akt/m-TOR pathway. PMID: 27886213
  4. CTGF and BMP2 are induced following myocardial ischemia in mice and humans. PMID: 28460577
  5. Results indicate that Notch signaling induces cell cycle arrest and thereby initiates chondrocyte cell enlargement via bone morphogenetic protein 2 (BMP2) signaling. PMID: 27146698
  6. Collectively, our findings indicate that CBFA2T2 is required for BMP-2-induced osteogenic differentiation of MSCs through inhibition of EHMT1-mediated histone methylation at Runx2 promoter. PMID: 29378183
  7. The data suggest that SDF-1beta provides synergistic effects supporting BMP-2-induced, BMSC-mediated bone formation and appears suitable for optimization of bone augmentation in combination therapy protocols. PMID: 26227988
  8. study revealed that only BMP-6 was able to induce bone formation at the used dose and that the addition of IGF-1 contributed to an increase of the mineralization in the implants. Hence, the combination of BMP-6 with IGF-1 might be a better alternative than BMP-2 for orthopedic surgery or bone tissue engineering approaches. PMID: 28256809
  9. BMP2 may induce osteogenic differentiation. PMID: 28395271
  10. Increased bone mass in Crmp4(-/-) mice was associated with enhanced BMP2 signaling and BMP2-induced osteoblast differentiation in Crmp4(-/-) osteoblasts (OBs). PMID: 28019696
  11. Deletion of the "ultra-conserved sequence" within the 3'UTR of the Bmp2 was associated with elevated Bmp2 mRNA and BMP signaling levels, reduced fitness, and embryonic malformations. PMID: 28401685
  12. miR-106b inhibited osteoblastic differentiation and bone formation partly through directly targeting bone morphogenetic protein 2. PMID: 28108317
  13. cells stimulated with BMP-2 in the presence of FBS require the phosphorylation of Akt at Ser473 and the dephosphorylation of Akt at Thr308 to increase the osteoblast differentiation with alkaline phosphatase activity similar to that of BMP-9 plus FBS. PMID: 27477105
  14. This study showed that release of BMP-2 and SDF-1alpha from heparinized MCM scaffolds allows for the reduction of the applied BMP-2 concentration since SDF-1alpha seems to enhance the osteoinductive potential of BMP-2. PMID: 27060915
  15. mice implanted with sulfonated PRX/BMP-2 complexes exhibited rapid and significant bone regeneration compared to those implanted with free BMP-2 and heparin/BMP-2 complexes. PMID: 28130833
  16. these data demonstrate that in addition to BMP6, endothelial cell BMP2 has a non-redundant role in hepcidin regulation by iron. PMID: 28815688
  17. results indicate that Alx3 expression is enhanced by BMP-2 via the BMP receptors mediated-Smad signaling and that Alx3 is a positive regulator of osteoblast differentiation induced by BMP-2 PMID: 23825702
  18. KDM5A-mediated H3K4me3 modification participated in the etiology of osteoporosis and may provide new strategies to improve the clinical efficacy of BMP2 in osteoporotic conditions. PMID: 27512956
  19. BMP2 expression in the endocardial lineage is essential for the distal outgrowth, maturation and remodeling of atrioventricular endocardial cushions. PMID: 28790014
  20. the effect of titanium (Ti) with nanotopography (Nano) on the endogenous expression of BMP-2 and BMP-4 and the relevance of this process to the nanotopography-induced osteoblast differentiation. PMID: 26681207
  21. Dynamin-dependent endocytosis of Bone Morphogenetic Protein2 (BMP2) and its receptors is dispensable for the initiation of Smad signaling PMID: 27113717
  22. can increase [Ca(2+) ]i through extracellular calcium influx regulated by FAK and ALP and can deplete endoplasmic reticulum calcium through Src signaling simultaneously PMID: 26890302
  23. Our studies suggest operation of an auto/paracrine mechanism by which BMP-2 secreted by M1 macrophages maintains constitutive activation of a BMP-2 receptor/SMAD1/5 signaling axis. PMID: 28711495
  24. Genetic inactivation of hepatic angiocrine Bmp2 signaling in Stab2-Cre mice caused massive iron overload in the liver and increased serum iron levels and iron deposition in several organs similar to classic hereditary hemochromatosis; these changes were mediated by decreased hepatic expression of hepcidin, a key regulator of iron homeostasis. PMID: 27903529
  25. Actin cytoskeleton depolymerization inhibites BMP2 signaling via blocking of Smad by dephosphorylated CNN1 in osteoblast cells under simulated microgravity. PMID: 28457943
  26. Thus, our findings identify an unrecognized function of neogenin in mouse neocortical astrocyte differentiation, and suggest a signaling pathway, BMP2-neogenin-YAP-Smad1, underlying astrogliogenesis in developing mouse neocortex. PMID: 27225772
  27. These results suggest that BMP-2 directly induces Ifrd1 expression at the transcriptional level in osteoblasts via the Smad pathway, and Ifrd1 negatively regulates BMP-2-dependent osteoblastogenesis. PMID: 27856249
  28. By suppressing BMP2 signaling the adverse effects of excessive inflammation after myocardial infarction are limited. PMID: 27283840
  29. Western blot analysis demonstrated that following BMP2 and BMP7 cotransfection of MC3T3E1 cells, the protein expression levels of BMP2, BMP4, BMP6, BMP7, BMP9 and Wnt3a were increased compared with control cells PMID: 27633082
  30. treatment of mBMSCs with the selective inhibitor of NF-kappaB pathway, BAY11-770682, showed to retrieve the inhibitory effect of CM-Adipo on BMP2-induced osteoblast differentiation in mBMSCs. PMID: 28173811
  31. BetA can enhance in vivo osteogenic potentials of BMP2, possibly via stimulating Smad 1/5/8 and p38 pathways, and combination of both agents can be considered as a therapeutic strategy for bone diseases. PMID: 27188281
  32. Mutant GDF5 enhances ameloblast differentiation via accelerated BMP2-induced Smad1-Smad5-Smad8 phosphorylation. PMID: 27030100
  33. Combining VEGF165 obviously enhanced the inducing effects of BMP2 on osteogenic differentiation capacity of C3H10T1/2 cells. PMID: 26757978
  34. Increased hepcidin in transferrin-treated thalassemic mice correlates with increased liver BMP2 expression and decreased hepatocyte ERK activation. PMID: 26635037
  35. Studied the effect of 7-dehydrocholesterol reductase (Dhcr7) and identified signal pathways involved in regulation of embryonic palatogenesis. Expression changes of Dhcr7, Sonic Hedgehog (Shh), and bone morphogenetic protein-2 (Bmp2) were measured by RT-PCR and WB after Dhcr7 gene silencing and the addition of exogenous cholesterol. PMID: 27066502
  36. A specific conserved AU-rich element within the Bmp2 ultra-conserved sequence was directly bound by the activating protein HuR. PMID: 26212702
  37. Mesenchymal Stem Cells Within Gelatin/CaSO4 Scaffolds Treated Ex Vivo with Low Doses of BMP-2 and Wnt3a Increase Bone Regeneration. PMID: 26414873
  38. Following fracture, a CXCL12(+)-BMP2(+) perivascular cell population is recruited along the endosteum. PMID: 25967044
  39. Cyclic stretch enhanced the BMP-2induced osteoblastic differentiation through the inhibition of Hey1. PMID: 26647760
  40. Regulatory effects of fibroblast growth factor-8 and tumor necrosis factor-alpha on osteoblast marker expression induced by bone morphogenetic protein-2. PMID: 26409788
  41. assessed the role of BMP2 expression globally, by osteoblasts, and by vascular endothelial cells in endochondral healing, intramembranous healing and lamellar bone formation PMID: 26344756
  42. Combination of bone morphogenetic protein-2 plasmid DNA with chemokine CXCL12 creates an additive effect on bone formation PMID: 26214286
  43. these results suggest that the recently described nuclear variant of BMP2 is necessary for efficient secondary immune responses. PMID: 26491697
  44. only BMP7, not BMP2 or BMP4, is necessary for interdigital programmed cell death PMID: 26826495
  45. Gremlin blocks BMP2 signaling and function in pancreatic stellate cells in vitro PMID: 26141517
  46. These results demonstrated that the cotransfection of BMP-2 and VEGF into microencapsulated C2C12 cells is of potent utility for the potentiation of bone regeneration. PMID: 26451370
  47. alphavbeta3 integrin is required to mediate BMP-2-induced Smad signaling. PMID: 26953352
  48. Report phenotype of BMP2/4 double knockout osteoblast cell line. PMID: 26595646
  49. integrin alpha1beta1/BMPR IA may block BMP-2/BMPR IA complex information and interfere with the BMP-2 signalling pathway in cells PMID: 26475222
  50. Decreased expression of Osterix, as well as impaired TGFbeta and BMP2 signaling, contribute to the observed osteopenic bone phenotype of TIEG1 KO mice. PMID: 26801561

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Subcellular Location Secreted
Protein Families TGF-beta family
Database Links

KEGG: mmu:12156

STRING: 10090.ENSMUSP00000028836

UniGene: Mm.103205

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