Mouse Bone morphogenetic protein 2,BMP-2 ELISA Kit

Code CSB-E04509m
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Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. P21274
Lead Time 3-5 working days
Abbreviation BMP2
Protein Biological Process 1 Developmental Protein
Target Name bone morphogenetic protein 2
Alias BMP2A,
Species Mus musculus (Mouse)
Protein Biological Process 3 Chondrogenesis
Sample Types serum, plasma, cell culture supernates, tissue homogenates
Detection Range 1.56 pg/mL-100 pg/mL
Sensitivity 0.39 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details This protein belongs to the transforming growth factor-beta (TGFB) superfamily. The encoded protein acts as a disulfide-linked homodimer and induces bone and cartilage formation.
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse BMP-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 97  
Range % 88-101  
1:2 Average % 101  
Range % 95-105  
1:4 Average % 90  
Range % 85-94  
1:8 Average % 99  
Range % 91-103  
Recovery
The recovery of mouse BMP-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 88 80-92  
EDTA plasma (n=4) 101 92-107  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
100 2.643 2.453 2.548 2.423  
50 2.024 1.976 2.000 1.875  
25 1.349 1.312 1.331 1.206  
12.5 0.742 0.724 0.733 0.608  
6.25 0.449 0.467 0.458 0.333  
3.12 0.307 0.300 0.304 0.179  
1.56 0.242 0.237 0.240 0.115  
0 0.126 0.124 0.125    
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Function Induces cartilage and bone formation. Stimulates the differentiation of myoblasts into osteoblasts via the EIF2AK3-EIF2A- ATF4 pathway. BMP2 activation of EIF2AK3 stimulates phosphorylation of EIF2A which leads to increased expression of ATF4 which plays a central role in osteoblast differentiation. In addition stimulates TMEM119, which upregulates the expression of ATF4.
Subcellular Location Secreted
Protein Families TGF-beta family
Database Links

KEGG: mmu:12156

STRING: 10090.ENSMUSP00000028836

UniGene: Mm.103205

Pathway Hippo signaling pathway
TGF-beta signaling pathway

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