Mouse Cardiotrophin-like cytokine factor 1(CLCF1) ELISA kit

Code CSB-EL005479MO
Size 96T,5×96T,10×96T
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Product Details

Target Name
cardiotrophin-like cytokine factor 1
Alternative Names
Clcf1 ELISA kit; Bsf3 ELISA kit; Nnt1Cardiotrophin-like cytokine factor 1 ELISA kit; B-cell-stimulating factor 3 ELISA kit; BSF-3 ELISA kit; Novel neurotrophin-1 ELISA kit; NNT-1 ELISA kit
Abbreviation
CLCF1
Uniprot No.
Species
Mus musculus (Mouse)
Sample Types
serum, plasma, cell culture supernates, tissue homogenates
Detection Range
23.5 pg/mL-1500 pg/mL
Sensitivity
5.8 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Others
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse CLCF1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 90
Range % 85-94
1:2 Average % 89
Range % 85-93
1:4 Average % 103
Range % 98-109
1:8 Average % 85
Range % 80-90
Recovery
The recovery of mouse CLCF1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 96 91-100
EDTA plasma (n=4) 91 85-97
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
1500 2.401 2.293 2.347 2.204
750 1.927 1.876 1.902 1.759
375 1.315 1.361 1.338 1.195
187.5 0.869 0.837 0.853 0.710
94 0.574 0.584 0.579 0.436
47 0.338 0.319 0.329 0.186
23.5 0.232 0.245 0.239 0.096
0 0.146 0.140 0.143  
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Mouse CLCF1 ELISA Kit was designed for the quantitative measurement of Mouse CLCF1 protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 23.5 pg/mL-1500 pg/mL and the sensitivity is 5.8 pg/mL.

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Target Background

Function
(From Uniprot)
In complex with CRLF1, forms a heterodimeric neurotropic cytokine that plays a crucial role during neuronal development. Also stimulates B-cells. Binds to and activates the ILST/gp130 receptor.
Gene References into Functions
  1. Cardiotrophin-like cytokine factor 1 (CLCF1) and neuropoietin (NP) signalling and their roles in development, adulthood, cancer and degenerative disorders. PMID: 26198769
  2. CLC plays a role in astrocyte differentiation via STAT3 activation within the developing brain PMID: 12090754
  3. IL-6 (gp130) family cytokine members include CLC/NNT1/BSF3 and IL-27 [review] PMID: 15253382
  4. In this study, Clc knockout mice had decreased facial motility, did not suckle, died within 24 hours, and had 32% and 29% fewer motor neurons in the facial nucleus and lumbar ventral horn, respectively. PMID: 19098279
Subcellular Location
Secreted.
Protein Families
IL-6 superfamily
Database Links
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