Mouse Collagen Type Ⅲ,Col Ⅲ ELISA KIT

Mouse Collagen Type Ⅲ,Col Ⅲ ELISA KIT

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Citations

Customer Reviews and Q&A

Product Details

Citations

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Mouse Collagen Type Ⅲ,Col Ⅲ ELISA KIT

Citations (7) Protocol MSDS

Code	CSB-E07925m
Size	96T,5×96T,10×96T
Price	Request a Quote
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Product Details
Citations (7)
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Customer Reviews and Q&A

Target Name

Collagen Type Ⅲ,Col Ⅲ

Alternative Names

Collagen Type 3,Col 3,Collagen Type III,Col III

Abbreviation

Col Ⅲ

Species

Mus musculus (Mouse)

Sample Types

serum, plasma

Detection Range

3.12 pg/mL-200 pg/mL

Sensitivity

0.78 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Signal Transduction

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse Col Ⅲ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:20	Average %	89
	Range %	82-96
1:40	Average %	98
	Range %	94-102
1:80	Average %	97
	Range %	90-104
1:160	Average %	86
	Range %	82-90

Recovery

The recovery of mouse Col Ⅲ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	87-99
EDTA plasma (n=4)	100	96-104

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
200	2.553	2.546	2.550	2.357
100	1.875	1.954	1.915	1.722
50	1.297	1.306	1.302	1.109
25	0.832	0.875	0.854	0.661
12.5	0.513	0.556	0.535	0.342
6.25	0.375	0.395	0.385	0.192
3.12	0.246	0.253	0.250	0.057
0	0.188	0.197	0.193

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.

Description

This Mouse Col Ⅲ ELISA Kit was designed for the quantitative measurement of Mouse Col Ⅲ protein in serum, plasma. It is a Sandwich ELISA kit, its detection range is 3.12 pg/mL-200 pg/mL and the sensitivity is 0.78 pg/mL.

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.