Mouse Collagen alpha-1(V) chain (COL5A1) ELISA kit

Instructions
Code CSB-EL005748MO
Size 96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name collagen, type V, alpha 1
Alternative Names Col5a1Collagen alpha-1(V) chain ELISA kit
Abbreviation COL5A1
Uniprot No. O88207
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates
Detection Range 0.312 ng/ml -20 ng/ml
Sensitivity 0.078 ng/ml
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 7-14 working days

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 Q&A
Q:

Per customers request, please provide the following information regarding Mouse Collagen alpha-1 (V) chain, COL5A1 ELISA Kit CSB-EL005748MO
1. Please provide current manual
2. If competitive please provide following information:
a. What is the standard?
b. What is the immunogen this kit detects? Sequence Range?
c. What is the antibody in this kit?
3. If Sandwich please provide the following information:
a. What is the standard? (Recombinant full-length protein? Partial protein (range?)?, peptide (sequence range?), native protein?)
b. If recombinant protein, then what is the host?
c. Is the capture antibody a monoclonal or polyclonal?
d. What is the host of the capture antibody?
e. What is the immunogen of the capture antibody?
f. Is the detection antibody a monoclonal or polyclonal?
g. What is the host of the detection antibody?
h. What is the immunogen of the detection antibody?

A:
Thanks for your inquiry!
CSB-EL005748MO
Pls find attached manual.
This kit is based on double antibody sandwich method. Pls refer to: https://www.uniprot.org/uniprot/O88207.
Capture antibody is rabbit polyclonal antibody and detection antibody is rabbit polyclonal antibody.
Immunogen is 293 cell-derived recombinant protein.
Standard is 293 cell-derived recombinant protein.

Target Background

Function
(From Uniprot)
Type V collagen is a member of group I collagen (fibrillar forming collagen). It is a minor connective tissue component of nearly ubiquitous distribution. Type V collagen binds to DNA, heparan sulfate, thrombospondin, heparin, and insulin (By similarity). Transcriptionally activated by CEBPZ, which recognizes a CCAAT-like motif, CAAAT in the COL5A1 promoter.
Gene References into Functions
  1. COL5A1 may contribute the metastasis of lung adenocarcinoma. PMID: 29702185
  2. Significantly decreased fibroblast proliferation was observed in Col5a1(+/-) compared to Col5a1(+/+) fibroblasts. PMID: 26713685
  3. Col5a1 knockout stroma demonstrated severe dysfunctional regulation of fibrillogenesis. PMID: 22159420
  4. Sp7/Osterix was observed to participate in the activation of the Col5a1 promoter during osteoblast differentiation. PMID: 20888414
  5. Col(V) is an established autoantigen in atherosclerotic mice, and Col(V) autoimmunity is a consistent feature, with variable T helper type (Th)1 cell pathway involvement. PMID: 20814021
  6. analysis of processing of the Pro-alpha1(V)Pro-alpha2(V)Pro-alpha3(V) procollagen heterotrimer PMID: 15136578
  7. CBF activated the Col5a1 promoter suggesting that CBF is a key factor in the coordinated expression of type I and V collagen genes PMID: 15246108
  8. collagen type V has a critical role in early fibril initiation PMID: 15383546
  9. COL5A1 is regulated by TGF-beta in osteoblasts PMID: 15579311
  10. col5a1 haploinsufficiency disrupts collagen fibril assembly at multiple stages PMID: 16492673
  11. Type V collagen contributes to the development of functional connective tissues during embryogenesis. PMID: 17024418
  12. ColVa1+/- and ColXIa1-/- mutants were used to identify roles for ColVa1 and ColXIa1 in ventricular myocardial morphogenesis and heart valve development. PMID: 17029294

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Subcellular Location Secreted, extracellular space, extracellular matrix
Protein Families Fibrillar collagen family
Tissue Specificity Expressed in limbs, vertebrae, heart, brain, liver, intestine, tongue, tail, skin, calvaria, lung and kidney of E18 embryos.
Database Links

KEGG: mmu:12831

STRING: 10090.ENSMUSP00000028280

UniGene: Mm.7281

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