Mouse Collagen alpha-1(V) chain (COL5A1) ELISA kit

Code CSB-EL005748MO
Size 96T
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Product Details

Target Name
collagen, type V, alpha 1
Alternative Names
Col5a1Collagen alpha-1(V) chain ELISA kit
Abbreviation
COL5A1
Uniprot No.
Species
Mus musculus (Mouse)
Sample Types
serum, plasma, tissue homogenates
Detection Range
0.312 ng/ml -20 ng/ml
Sensitivity
0.078 ng/ml
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Signal Transduction
Assay Principle
quantitative
Measurement
Sandwich
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx

Customer Reviews and Q&A

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 Q&A
Q:

Per customers request, please provide the following information regarding Mouse Collagen alpha-1 (V) chain, COL5A1 ELISA Kit CSB-EL005748MO
1. Please provide current manual
2. If competitive please provide following information:
a. What is the standard?
b. What is the immunogen this kit detects? Sequence Range?
c. What is the antibody in this kit?
3. If Sandwich please provide the following information:
a. What is the standard? (Recombinant full-length protein? Partial protein (range?)?, peptide (sequence range?), native protein?)
b. If recombinant protein, then what is the host?
c. Is the capture antibody a monoclonal or polyclonal?
d. What is the host of the capture antibody?
e. What is the immunogen of the capture antibody?
f. Is the detection antibody a monoclonal or polyclonal?
g. What is the host of the detection antibody?
h. What is the immunogen of the detection antibody?

A:
Thanks for your inquiry!
CSB-EL005748MO
Pls find attached manual.
This kit is based on double antibody sandwich method. Pls refer to: https://www.uniprot.org/uniprot/O88207.
Capture antibody is rabbit polyclonal antibody and detection antibody is rabbit polyclonal antibody.
Immunogen is 293 cell-derived recombinant protein.
Standard is 293 cell-derived recombinant protein.

Target Background

Function
(From Uniprot)
Type V collagen is a member of group I collagen (fibrillar forming collagen). It is a minor connective tissue component of nearly ubiquitous distribution. Type V collagen binds to DNA, heparan sulfate, thrombospondin, heparin, and insulin. Transcriptionally activated by CEBPZ, which recognizes a CCAAT-like motif, CAAAT in the COL5A1 promoter.
Gene References into Functions
  1. COL5A1 may contribute the metastasis of lung adenocarcinoma. PMID: 29702185
  2. Significantly decreased fibroblast proliferation was observed in Col5a1(+/-) compared to Col5a1(+/+) fibroblasts. PMID: 26713685
  3. Col5a1 knockout stroma demonstrated severe dysfunctional regulation of fibrillogenesis. PMID: 22159420
  4. Sp7/Osterix was observed to participate in the activation of the Col5a1 promoter during osteoblast differentiation. PMID: 20888414
  5. Col(V) is an established autoantigen in atherosclerotic mice, and Col(V) autoimmunity is a consistent feature, with variable T helper type (Th)1 cell pathway involvement. PMID: 20814021
  6. analysis of processing of the Pro-alpha1(V)Pro-alpha2(V)Pro-alpha3(V) procollagen heterotrimer PMID: 15136578
  7. CBF activated the Col5a1 promoter suggesting that CBF is a key factor in the coordinated expression of type I and V collagen genes PMID: 15246108
  8. collagen type V has a critical role in early fibril initiation PMID: 15383546
  9. COL5A1 is regulated by TGF-beta in osteoblasts PMID: 15579311
  10. col5a1 haploinsufficiency disrupts collagen fibril assembly at multiple stages PMID: 16492673
  11. Type V collagen contributes to the development of functional connective tissues during embryogenesis. PMID: 17024418
  12. ColVa1+/- and ColXIa1-/- mutants were used to identify roles for ColVa1 and ColXIa1 in ventricular myocardial morphogenesis and heart valve development. PMID: 17029294

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Subcellular Location
Secreted, extracellular space, extracellular matrix.
Protein Families
Fibrillar collagen family
Tissue Specificity
Widely expressed. Isoform 2 is more highly expressed in liver, kidney and lung.
Database Links
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