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Code | CSB-E07974m |
Size | 96T,5×96T,10×96T |
Price | Request a Quote or Start an on-line Chat |
Trial Size |
24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now |
Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
Inter-assay Precision (Precision between assays): CV%<10% | ||||||
Three samples of known concentration were tested in twenty assays to assess. | ||||||
To assess the linearity of the assay, samples were spiked with high concentrations of mouse COL18A1/ES in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
Sample | Serum(n=4) | |||||
1:1 | Average % | 92 | ||||
Range % | 89-95 | |||||
1:2 | Average % | 103 | ||||
Range % | 99-105 | |||||
1:4 | Average % | 92 | ||||
Range % | 87-96 | |||||
1:8 | Average % | 99 | ||||
Range % | 94-101 |
The recovery of mouse COL18A1/ES spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
Sample Type | Average % Recovery | Range | ||||
Serum (n=5) | 95 | 91-98 | ||||
EDTA plasma (n=4) | 95 | 91-98 | ||||
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
ng/ml | OD1 | OD2 | Average | Corrected | ||
10 | 2.234 | 2.213 | 2.224 | 2.067 | ||
5 | 1.732 | 1.694 | 1.713 | 1.556 | ||
2.5 | 1.091 | 1.076 | 1.084 | 0.927 | ||
1.25 | 0.551 | 0.537 | 0.544 | 0.387 | ||
0.625 | 0.366 | 0.386 | 0.376 | 0.219 | ||
0.312 | 0.292 | 0.281 | 0.287 | 0.130 | ||
0.156 | 0.248 | 0.228 | 0.238 | 0.081 | ||
0 | 0.159 | 0.155 | 0.157 | |||
This Mouse COL18A1/ES ELISA Kit was designed for the quantitative measurement of Mouse COL18A1/ES protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.156 ng/mL-10 ng/mL and the sensitivity is 0.039 ng/mL .
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