Mouse Complement C1q tumor necrosis factor-related protein 3(C1QTNF3) ELISA kit

Instructions
Code CSB-EL003648MO
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name C1q and tumor necrosis factor related protein 3
Alternative Names C1qtnf3 ELISA Kit; Cors26 ELISA Kit; Ctrp3 ELISA Kit; Complement C1q tumor necrosis factor-related protein 3 ELISA Kit; Collagenous repeat-containing sequence 26 kDa protein ELISA Kit; CORS26 ELISA Kit; Secretory protein CORS26 ELISA Kit
Abbreviation C1QTNF3
Uniprot No. Q9ES30
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates
Detection Range 3.12 ng/mL-200 ng/mL
Sensitivity 0.78 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Others
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse C1QTNF3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 92
Range % 87-96
1:2 Average % 101
Range % 97-106
1:4 Average % 93
Range % 86-97
1:8 Average % 91
Range % 87-95
Recovery
The recovery of mouse C1QTNF3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 96 92-101
EDTA plasma (n=4) 98 94-102
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected
200 2.123 2.268 2.196 2.045
100 1.478 1.548 1.513 1.362
50 0.941 0.958 0.950 0.799
25 0.586 0.597 0.592 0.441
12.5 0.412 0.403 0.408 0.257
6.25 0.302 0.309 0.306 0.155
3.12 0.218 0.224 0.221 0.070
0 0.150 0.151 0.151  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 7-14 working days

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Target Background

Gene References into Functions
  1. Low C1QTNF3 expression is associated with polycystic ovarian syndrome. PMID: 29438034
  2. Although it is dispensable for physiological control of energy balance, CTRP3 plays a previously unsuspected role in modulating liver size and circulating cytokine levels in response to obesity. PMID: 26670485
  3. CTRP3 was expressed in developing skeletal muscle tissues, and the expression level of CTRP3 was increased during myogenic differentiation of C2C12 cells. PMID: 26272338
  4. Our study suggests that CTRP3 prevents osteoclast differentiation PMID: 26103094
  5. Data suggest that CTRP3 up-regulates expression and secretion of adipokines (adiponectin, leptin, visfatin, and apelin) in adipocytes; these responses to CTRP3 are down-regulated by insulin resistance or inhibition of AMPK signaling pathway. PMID: 25168658
  6. present study shows the proof of principle that the novel adipokine CTRP-3 is a potent inhibitor of LPS-induced systemic inflammation and LPS-induced signaling in adipose tissue in vivo PMID: 24996172
  7. CTRP3 promotes vascular calcification by enhancing phosphate-induced osteogenic transition of VSMC through reactive oxygen species-extracellular signal-regulated kinase 1/2-Runx2 pathway. PMID: 24578384
  8. These observations indicate that CTRP3 plays an important role in the development of autoimmune arthritis, suggesting CTRP3 as a possible medicine to treat rheumatoid arthritis. PMID: 24269820
  9. results establish a novel role for C1q tumor necrosis factor-related protein 3 (CTRP3) in regulating hepatic lipid metabolism and highlight its protective function and therapeutic potential in attenuating hepatic steatosis PMID: 23744740
  10. c-Jun is a cis-acting element for CTRP3 regulation in chondrogenic cells. PMID: 22644487
  11. CTRP3 is a novel antiapoptotic, proangiogenic, and cardioprotective adipokine, the expression of which is significantly inhibited after myocardial infarction. PMID: 22653084
  12. This study provides the first functional evidence linking CTRP3 to hepatic glucose metabolism and establishes CTRP3 as a novel adipokine. PMID: 20952387
  13. Exogenous CTRP3/cartducin promoted the proliferation of p53LMAC01 cells in a dose-dependent manner via ERK1/2 (extracellular signal-regulated kinase 1/2)- and MAPK (p38 mitogen-activated protein kinase)-signalling pathways PMID: 19947921
  14. Genomic organization, chromosomal localization and adipocytic expression of the murine gene for CORS-26 PMID: 12850274
  15. the murine CORS-26 promoter is transcriptionally regulated by specificity protein-1, PPARgamma, and pituitary protein transcription factor-1 PMID: 15157741
  16. Cartducin is a novel growth factor and plays important roles in regulating both chondrogenesis and cartilage development by its direct stimulatory action on the proliferation of chondrogenic precursors and chondrocytes. PMID: 16155912
  17. CORS-26 up-regulates adipokine secretion and might be involved in metabolic and immunologic pathways. PMID: 17299102
  18. Elevated expression of CTRP3/cartducin contributes to promotion of osteosarcoma cell proliferation PMID: 19424626

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Subcellular Location Secreted
Database Links

KEGG: mmu:81799

STRING: 10090.ENSMUSP00000106152

UniGene: Mm.280158

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