Mouse Keratin, type I cytoskeletal 18 fragment (K18-Asp396)ELISA Kit

Code CSB-E14265m
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Description

This Mouse CK 18-M30 ELISA Kit was designed for the quantitative measurement of Mouse CK 18-M30 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 15.6 μIU/mL-1000 μIU/mL and the sensitivity is 3.9 μIU/mL.

Target Name Cytokeratin 18-M30(CK 18-M30)
Abbreviation CK 18-M30
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates
Detection Range 15.6 μIU/mL-1000 μIU/mL
Sensitivity 3.9 μIU/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Others
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse CK 18-M30 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:100 Average % 96  
Range % 89-104  
1:200 Average % 95  
Range % 90-100  
1:400 Average % 92  
Range % 87-101  
1:800 Average % 93  
Range % 90-97  
Recovery
The recovery of mouse CK 18-M30 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 95 89-99  
EDTA plasma (n=4) 96 90-104  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
µIU/ml OD1 OD2 Average Corrected  
1000 2.317 2.217 2.267 2.164  
500 1.467 1.367 1.417 1.314  
250 0.797 0.777 0.787 0.684  
125 0.412 0.392 0.402 0.299  
62.5 0.268 0.258 0.263 0.160  
31.2 0.199 0.189 0.194 0.091  
15.6 0.165 0.164 0.165 0.062  
0 0.103 0.102 0.103    
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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