Mouse Disintegrin and metalloproteinase domain-containing protein 33(ADAM33) ELISA kit

Code CSB-EL001290MO
Size 96T,5×96T,10×96T
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Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details

Target Name
ADAM metallopeptidase domain 33
Alternative Names
Adam33Disintegrin and metalloproteinase domain-containing protein 33 ELISA kit; ADAM 33 ELISA kit; EC 3.4.24.- ELISA kit
Abbreviation
ADAM33
Uniprot No.
Species
Mus musculus (Mouse)
Sample Types
serum, plasma, tissue homogenates
Detection Range
15.6 pg/mL-1000 pg/mL
Sensitivity
3.9 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Cell Biology
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse ADAM33 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %93
Range %88-97
1:2Average %84
Range %80-88
1:4Average %95
Range %90-100
1:8Average %104
Range %99-109
Recovery
The recovery of mouse ADAM33 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9085-94
EDTA plasma (n=4)9287-96
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
10002.658 2.552 2.605 2.434
5001.881 2.006 1.944 1.773
2501.128 1.204 1.166 0.995
1250.627 0.653 0.640 0.469
62.50.372 0.401 0.387 0.216
31.20.276 0.264 0.270 0.099
15.60.234 0.239 0.237 0.066
00.174 0.167 0.171
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Mouse ADAM33 ELISA Kit was designed for the quantitative measurement of Mouse ADAM33 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL.

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Target Background

Gene References into Functions
  1. The mouse and human predicted proteins consisted of 797 and 813 amino acids, respectively, and they shared 70% homology of the entire amino acid sequence. PMID: 11814695
  2. Identification and preliminary characterization of mouse Adam33. PMID: 11897009
  3. ADAM33 is not essential for growth or reproduction in the mouse and does not modulate baseline or allergen-induced airway responsiveness. PMID: 16943435
  4. Adam33/Adam33 shows 2 significant increments in expression during lung morphogenesis, suggesting important developmental regulation. PMID: 19665773
Subcellular Location
Membrane; Single-pass type I membrane protein.
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