Mouse Epidermal growth factor-like protein 6(EGFL6) ELISA kit

Instructions
Code CSB-EL007475MO
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name EGF-like-domain, multiple 6
Alternative Names Egfl6 ELISA Kit; Maeg ELISA Kit; Epidermal growth factor-like protein 6 ELISA Kit; EGF-like protein 6 ELISA Kit; MAM and EGF domains-containing gene protein ELISA Kit; Protein W80 ELISA Kit
Abbreviation EGFL6
Uniprot No. Q9JJZ5
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates
Detection Range 93.75 pg/mL-6000 pg/mL
Sensitivity 23.43 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse EGFL6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 90  
Range % 85-94  
1:2 Average % 98  
Range % 94-101  
1:4 Average % 102  
Range % 94-108  
1:8 Average % 94  
Range % 87-97  
Recovery
The recovery of mouse EGFL6 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 97 94-103  
EDTA plasma (n=4) 93 89-96  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
6000 2.414 2.355 2.385 2.297  
3000 1.612 1.564 1.588 1.500  
1500 1.052 1.090 1.071 0.983  
750 0.688 0.633 0.661 0.573  
375 0.459 0.414 0.437 0.349  
187.5 0.298 0.280 0.289 0.201  
93.75 0.175 0.165 0.170 0.082  
0 0.088 0.087 0.088    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 5-7 working days

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Target Background

Function
(From Uniprot)
May bind integrin alpha-8/beta-1 and play a role in hair follicle morphogenesis. Promotes matrix assembly.
Gene References into Functions
  1. EGLF6 mediates a paracrine mechanism of cross-talk between vascular endothelial cells and osteoblasts PMID: 21531721
  2. MAEG may play a role as a mediator regulating epithelial-mesenchymal interaction through binding to RGD-binding integrins including alpha8beta1 during hair follicle development. PMID: 15572035
Subcellular Location Secreted, extracellular space, extracellular matrix, basement membrane
Protein Families Nephronectin family
Tissue Specificity Expressed at basement membrane of pelage follicles (at protein level).
Database Links

KEGG: mmu:54156

UniGene: Mm.37707

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