Mouse Ferritin heavy chain(FTH1) ELISA kit

Instructions
Code CSB-EL009030MO
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name ferritin, heavy polypeptide 1
Alternative Names Fth1 ELISA Kit; FthFerritin heavy chain ELISA Kit; Ferritin H subunit ELISA Kit; EC 1.16.3.1) [Cleaved into: Ferritin heavy chain ELISA Kit; N-terminally processed] ELISA Kit
Abbreviation FTH1
Uniprot No. P09528
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates
Detection Range 0.312 ng/mL-20 ng/mL
Sensitivity 0.078 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Metabolism
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse FTH1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 94  
Range % 88-99  
1:2 Average % 97  
Range % 92-103  
1:4 Average % 85  
Range % 81-88  
1:8 Average % 89  
Range % 83-95  
Recovery
The recovery of mouse FTH1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 92 87-97  
EDTA plasma (n=4) 98 95-104  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected  
20 2.178 2.162 2.170 2.012  
10 1.713 1.695 1.704 1.546  
5 1.085 1.128 1.107 0.949  
2.5 0.693 0.751 0.722 0.564  
1.25 0.397 0.388 0.393 0.235  
0.625 0.254 0.267 0.261 0.103  
0.312 0.193 0.201 0.197 0.039  
0 0.154 0.161 0.158    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 5-7 working days

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Target Data

Function Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Has ferroxidase activity. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation. Also plays a role in delivery of iron to cells. Mediates iron uptake in capsule cells of the developing kidney.
Gene References into Functions
  1. FtMt overexpressing mice have no significant defects and the overexpression of FtMt does not affect the regulation of iron metabolism significantly in transgenic mice. PMID: 28703745
  2. Ferritin is considered the major iron storage protein which maintains a large iron core in its cavity and has ferroxidase activity. (Review) PMID: 28349628
  3. the absence of Mitochondrial ferritin, which is highly expressed in the heart, increases the sensitivity of mitochondria to cardiac injury via oxidative stress. PMID: 27853170
  4. As previously reported, homozygous loss of the Fth allele on a wild-type Ftl background was embryonic lethal. expression of the H subunit can rescue the loss of the L subunit and that H ferritin homopolymers have the capacity to sequester iron in vivo PMID: 25629408
  5. provide a new mechanism for selective autophagy of ferritin and reveal a previously unappreciated role for autophagy and NCOA4 in the control of iron homeostasis in vivo PMID: 25327288
  6. Our data show that ferritin H is required for B and T cell survival by actively reducing the labile iron pool. PMID: 24586648
  7. Ferritin H deficiency in most cells of the forebrain including cells of the choroid plexus caused accumulation of cerebrospinal fluid in the lateral ventricles and the subarachnoid space PMID: 24662374
  8. Mycobacterium avium infection induces H-ferritin expression in mouse primary macrophages by activating Toll-like receptor 2. PMID: 24349383
  9. 3-Hydroxybutyrate dehydrogenase-2 and ferritin-H synergistically regulate intracellular iron. PMID: 24673886
  10. Fth plays a critical protective role during acute kidney injury. PMID: 24018561
  11. FtH provides metabolic adaptation to tissue Fe overload, conferring tolerance to malaria PMID: 23159058
  12. The delivery of iron to oligodendrocytes via H-ferritin is a biologically relevant delivery system, given that ferritin--not transferrin--receptors can be demonstrated on oligodendrocytes in vivo. PMID: 21446040
  13. HFt taken up by TIM-2 positive cells transited through the endosome and eventually entered a lysosomal compartment, distinguishing the HFt pathway from that of transferrin PMID: 21886823
  14. Mitochondrial ferritin is found in all regions of the brain in C57/BL6 mice; the role of mitochondrial ferritin can be expected to contribute to our knowledge of mitochondrial dysfunction and neurodegenerative disease. PMID: 20722075
  15. Loss of one allele of H ferritin appears to increase light-induced degeneration. PMID: 20881284
  16. Hx plays an important role in controlling iron distribution within brain, thus suggesting its involvement in iron-related neurodegenerative diseases. PMID: 19120692
  17. FTH expression is mediated by Nrf2 in response to xenobiotics and cancer chemopreventive dithiolethiones PMID: 12435735
  18. Ferritin heavy chain 1 necessary for tumor necrosis factor alpha-induced apoptosis in L929 cells. PMID: 16024802
  19. excessive free iron and the resultant enhanced oxidative stress caused by downregulation of FHC lead to cardiomyocyte death PMID: 18992754

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Protein Families Ferritin family
Database Links

KEGG: mmu:14319

STRING: 10090.ENSMUSP00000025563

UniGene: Mm.1776

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