Mouse Fibrinogen-like protein 1(FGL1) ELISA kit

Instructions
Code CSB-EL008653MO
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name fibrinogen-like 1
Alternative Names Fgl1 ELISA Kit; Mfire1 ELISA Kit; Fibrinogen-like protein 1 ELISA Kit; Fibrinogen-related protein 1 ELISA Kit; Mfrep-1 ELISA Kit; Liver fibrinogen-related protein-1 ELISA Kit; Mfire-1 ELISA Kit
Abbreviation FGL1
Uniprot No. Q71KU9
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates
Detection Range 31.25 pg/mL-2000 pg/mL
Sensitivity 7.8 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cardiovascular
Assay Principle quantitative
Measurement Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse FGL1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:100Average %95
Range %91-99
1:200Average %84
Range %81-89
1:400Average %83
Range %90-96
1:800Average %87
Range %84-90
Recovery
The recovery of mouse FGL1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 104100-108
EDTA plasma (n=4)107104-110
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2Average
20000.076 0.079 0.078
10000.135 0.146 0.141
5000.206 0.220 0.213
2500.362 0.379 0.371
1250.593 0.586 0.590
62.50.901 0.932 0.917
31.251.213 1.236 1.225
02.054 2.098 2.076
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 7-14 working days

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Target Background

Function
(From Uniprot)
Has hepatocyte mitogenic activity.
Gene References into Functions
  1. The results suggest that Fgl1 acts as a tumor suppressor in hepatocellular cancer through an Akt-dependent mechanism and support its role as a potential therapeutic target in hepatocellular carcinoma. PMID: 26225745
  2. Hepassocin plays an important role in non-alcoholic fatty liver disease and induces hepatic lipid accumulation through an ERK1/2-dependent pathway. PMID: 23792031
  3. Fgl1 is a member of an emerging group of proteins with key roles in metabolism and liver regeneration. PMID: 23483972
  4. HNF1 binding site and HNF1alpha are critical to liver-specific expression of HPS, and down-regulation or loss of HNF1alpha causes, at least in part, the transcriptional down-regulation of HPS in HCC. PMID: 19304666
Subcellular Location Secreted
Tissue Specificity Liver specific.
Database Links

KEGG: mmu:234199

STRING: 10090.ENSMUSP00000034003

UniGene: Mm.277955

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