Mouse Galanin-like peptide(GALP) ELISA kit

Code CSB-EL009222MO
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details

Target Name
galanin-like peptide
Alternative Names
GalpGalanin-like peptide ELISA Kit
Abbreviation
GALP
Uniprot No.
Species
Mus musculus (Mouse)
Sample Types
serum, plasma, tissue homogenates
Detection Range
23.5 pg/mL-1500 pg/mL
Sensitivity
5.8 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Cardiovascular
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse GALP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 93
Range % 86-97
1:2 Average % 104
Range % 97-108
1:4 Average % 92
Range % 87-98
1:8 Average % 91
Range % 87-95
Recovery
The recovery of mouse GALP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 87 83-92
EDTA plasma (n=4) 94 87-98
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
1500 2.421 2.536 2.479 2.288
750 1.905 2.011 1.958 1.767
375 1.328 1.413 1.371 1.180
187.5 0.821 0.834 0.828 0.637
94 0.514 0.527 0.521 0.330
47 0.370 0.387 0.379 0.188
23.5 0.311 0.315 0.313 0.122
0 0.190 0.192 0.191  
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Mouse GALP ELISA Kit was designed for the quantitative measurement of Mouse GALP protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 23.5 pg/mL-1500 pg/mL and the sensitivity is 5.8 pg/mL.

Customer Reviews and Q&A

 Customer Reviews

There are currently no reviews for this product.

Submit a Review here

Target Background

Function
(From Uniprot)
Hypothalamic neuropeptide which binds to the G-protein-coupled galanin receptors (GALR1, GALR2 and GALR3). Involved in a large number of putative physiological functions in CNS homeostatic processes, including the regulation of gonadotropin-releasing hormone secretion.; Exhibits antimicrobial activity against Gram-negative bacterias, inducing bacterial membrane blebbing. Exhibits potent and dose-dependent vasoconstrictor and anti-edema activity in the cutaneous microvasculature, a physiologic effects which does not appear to be mediated via GALR1 or GALR2.
Gene References into Functions
  1. this study demonstrates anti-obesity effect of intranasal administration of galanin-like peptide in obese mice PMID: 27323911
  2. we investigated whether PI3K signalling in neurones expressing GALP plays a role in the transcriptional regulation of the GALP gene PMID: 24796383
  3. results indicate the GALP ICV administration can further promote energy metabolism when administered to spontaneously exercising mice PMID: 24055807
  4. Distinct expression of alarin, a splice variant of GALP, in the adult mouse brain demonstrates potential functions in reproduction and metabolism. PMID: 21647713
  5. hypothalamic GALP gene expression is controlled by leptin signals PMID: 12746327
  6. GALP may play a role in readjusting energy balance under changing nutritional circumstances PMID: 18775887

Show More

Hide All

Subcellular Location
Secreted.
Protein Families
Galanin family
Tissue Specificity
Isoform 2 is found in brain, thymus and skin. Isoform 2 is found in the skin, in pericytes covering microvascular arterioles and venules on their abluminal surfaces. In larger vessels, isoform 2 is expressed in layers of smooth muscle cells. Isoform 2 is
Database Links
icon of phone
Call us
301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
icon of address
Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
icon of social media
Join us with

Subscribe newsletter

Leave a message

* To protect against spam, please pass the CAPTCHA test below.
CAPTCHA verification
© 2007-2024 CUSABIO TECHNOLOGY LLC All rights reserved. 鄂ICP备15011166号-1