Mouse Galectin 3(GAL-3) ELISA Kit

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Mouse Galectin 3(GAL-3) ELISA Kit

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Protocol

Code	CSB-E14296m-IS
Size	96T,5×96T,10×96T
Price	Request a Quote
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Product Details
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Customer Reviews and Q&A
Target Background

Target Name

lectin, galactoside-binding, soluble, 3

Associated names

Lgals3 ELISA Kit; Galectin-3 ELISA Kit; Gal-3 ELISA Kit; 35 kDa lectin ELISA Kit; Carbohydrate-binding protein 35 ELISA Kit; CBP 35 ELISA Kit; Galactose-specific lectin 3 ELISA Kit; IgE-binding protein ELISA Kit; L-34 galactoside-binding lectin ELISA Kit; Laminin-binding protein ELISA Kit; Lectin L-29 ELISA Kit; Mac-2 antigen ELISA Kit

Abbreviation

LGALS3

Uniprot No.

P16110

Species

Mus musculus (Mouse)

Sample Types

serum, plasma, cell culture supernates, tissue homogenates

Detection Range

31.25 pg/mL-2000 pg/mL

Sensitivity

7.81 pg/mL

Assay Time

1-5h

Sample Loading Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Cell Biology

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse GAL-3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:1	Average %	95
	Range %	88-100
1:2	Average %	90
	Range %	84-94
1:4	Average %	98
	Range %	92-105
1:8	Average %	86
	Range %	81-93

Recovery

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.


pg/ml	OD1	OD2	Average	Corrected
2000	2.421	2.295	2.358	2.248
1000	1.689	1.674	1.682	1.572
500	1.034	0.989	1.012	0.902
250	0.600	0.623	0.611	0.501
125	0.361	0.361	0.361	0.251
62.5	0.239	0.242	0.240	0.131
31.25	0.180	0.178	0.179	0.069
0	0.107	0.112	0.110

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.

Description

This ELISA kit provides quantitative measurement of LGALS3 (Galectin-3) in mouse samples, supporting research into its roles in fibrosis, inflammation, and macrophage activation. Because Galectin-3 is both an intracellular mediator and a secreted signal that fluctuates dramatically between resting and inflammatory states, precise quantification across compartments is essential.

Detection Range: The 31.25–2000 pg/mL range captures Galectin-3 levels in mouse serum under both baseline and inflammatory conditions, where concentrations can rise sharply during fibrotic or cardiac injury models. Serum samples from disease models may require dilution, while conditioned media from unstimulated cells can often be measured neat.

Sensitivity: At 7.81 pg/mL, the kit detects Galectin-3 in low-abundance contexts such as conditioned media from resting macrophages or early-stage fibrotic tissue, where secreted levels remain minimal before robust immune activation.

Sample Compatibility: Validation across serum, plasma, cell culture supernates, and tissue homogenates allows researchers to track Galectin-3 from its intracellular stores in tissue through its secretion into circulation — enabling paired analyses in organ fibrosis or tumor microenvironment studies.

Assay Efficiency: The 1–5 hour protocol and 50–100 µL sample volume accommodate primary macrophage cultures and small-volume mouse bleeds, common constraints in murine Galectin-3 studies.

This kit is particularly suited for investigating cardiac fibrosis, hepatic inflammation, and tumor-associated macrophage polarization in mouse models.

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Function
(From Uniprot)

Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis. In the nucleus: acts as a pre-mRNA splicing factor. Involved in acute inflammatory responses including neutrophil activation and adhesion, chemoattraction of monocytes macrophages, opsonization of apoptotic neutrophils, and activation of mast cells. Together with TRIM16, coordinates the recognition of membrane damage with mobilization of the core autophagy regulators ATG16L1 and BECN1 in response to damaged endomembranes.

Subcellular Location

Cytoplasm. Nucleus. Secreted.

Tissue Specificity

The highest levels are found in activated macrophages.

Database Links

STRING: 10090.ENSMUSP00000114350

UniGene: Mm.248615

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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