Mouse Galectin 3(GAL-3) ELISA Kit

Instructions
Code CSB-E14296m
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name lectin, galactoside-binding, soluble, 3
Alternative Names Lgals3 ELISA Kit; Galectin-3 ELISA Kit; Gal-3 ELISA Kit; 35 kDa lectin ELISA Kit; Carbohydrate-binding protein 35 ELISA Kit; CBP 35 ELISA Kit; Galactose-specific lectin 3 ELISA Kit; IgE-binding protein ELISA Kit; L-34 galactoside-binding lectin ELISA Kit; Laminin-binding protein ELISA Kit; Lectin L-29 ELISA Kit; Mac-2 antigen ELISA Kit
Abbreviation LGALS3
Uniprot No. P16110
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates
Detection Range 0.312 ng/mL-20 ng/mL
Sensitivity 0.078 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cell Biology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse GAL-3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %91
Range %85-103
1:2Average %90
Range %82-99
1:4Average %92
Range %86-100
1:8Average %92
Range %89-103
Recovery
The recovery of mouse GAL-3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9690-101
EDTA plasma (n=4)9791-105
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
202.852 2.842 2.847 2.703
102.207 2.280 2.244 2.100
51.357 1.384 1.371 1.227
2.50.841 0.862 0.852 0.708
1.250.475 0.458 0.467 0.323
0.6250.283 0.297 0.290 0.146
0.3120.231 0.244 0.238 0.094
00.142 0.146 0.144
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Data

Function Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis. In the nucleus
Subcellular Location Cytoplasm, Nucleus, Secreted
Tissue Specificity The highest levels are found in activated macrophages.
Database Links

STRING: 10090.ENSMUSP00000114350

UniGene: Mm.248615

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