Mouse Glutamate decarboxylase 1(GAD1) ELISA kit

Instructions
Code CSB-EL009159MO
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name glutamate decarboxylase 1 (brain, 67kDa)
Alternative Names Gad1 ELISA Kit; Gad67Glutamate decarboxylase 1 ELISA Kit; EC 4.1.1.15 ELISA Kit; 67 kDa glutamic acid decarboxylase ELISA Kit; GAD-67 ELISA Kit; Glutamate decarboxylase 67 kDa isoform ELISA Kit
Abbreviation GAD1
Uniprot No. P48318
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 6.25 pg/mL-400 pg/mL
Sensitivity 1.56 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Neuroscience
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse GAD1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:5 Average % 102  
Range % 99-105  
1:10 Average % 95  
Range % 91-98  
1:20 Average % 88  
Range % 84-93  
1:40 Average % 99  
Range % 95-104  
Recovery
The recovery of mouseGAD1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 104 100-109  
EDTA plasma (n=4) 93 89-96  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
400 2.951 2.896 2.924 2.753  
200 2.124 1.960 2.042 1.871  
100 1.339 1.411 1.375 1.204  
50 0.842 0.902 0.872 0.701  
25 0.599 0.642 0.621 0.450  
12.5 0.354 0.370 0.362 0.191  
6.25 0.256 0.248 0.252 0.081  
0 0.175 0.166 0.171    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 5-7 working days

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Target Background

Function
(From Uniprot)
Catalyzes the production of GABA.
Gene References into Functions
  1. Our results demonstrate that GAD67 plays an important role in Alzheimer's disease pathology PMID: 29017573
  2. Results suggest age-dependent decrease of GAD65/67 mRNAs but normal densities of certain GABAergic interneurons in the Shank3 transgenic mice. PMID: 28400125
  3. These findings reveal that Th and Gad1 share a transcription regulatory mechanism that facilitates odorant-dependent regulation of dopamine and GABA expression levels. PMID: 28411275
  4. This study demonstrated that Alterations in hypoglossal motor neurons due to GAD67 deficiency in mice. PMID: 27956032
  5. Treadmill running prevented partial sciatic nerve ligation-induced reductions in GAD65/67 production, and, thus, GABA levels may be retained in interneurons and neuropils in the superficial dorsal horn. PMID: 27030712
  6. Gad1 knockdown mice have pronounced sensorimotor gating deficits, increased novelty-seeking and reduced fear extinction PMID: 25623945
  7. Glutamate decarboxylase expression may be a reliable proxy of altered GABAergic transmission. PMID: 26370162
  8. Activity deprivation due to TTX preferentially down-regulated GAD67. BDNF-induced increase in the GAD67 protein was markedly lower than that of GAD65, indicating that BDNF differentially regulates activity-dependent gene expression of the 2 isoforms. PMID: 26241953
  9. The results of this study indicated that the mice heterozygous for GAD67 deficiency primarily in PV neurons share several neurochemical and behavioral abnormalities with schizophrenia. PMID: 25904362
  10. Data demonstrate that GAD67 levels directly influence synaptic inhibition. PMID: 24275833
  11. The GAD65-independent mechanism for targeting of GAD67 to synaptic vesicles in neurons is not functional in islet beta-cells. PMID: 25647668
  12. Results indicate the significant contribution of not only GAD65, GAD67 and VGAT-mediated GABAergic but also glycinergic transmissions to both palate and abdominal wall formations PMID: 25545713
  13. These findings provide original evidence that striatal Gad67 expression is involved in the modulation of learning and social behavior. PMID: 24952328
  14. Analysis of GAD65/67 single and double knock-out embryos revealed that the two GADs play complementary (inhibitory) roles in GnRH migration ultimately modulating the speed and/or direction of GnRH migration. PMID: 25125027
  15. Study reports the identification and characterization of novel GAD1 splicing isoforms, showing GAD1 gene in mouse brain is extensively regulated at the level of transcription PMID: 25322942
  16. Gad67-GFP mice results in a sex-dependent deregulation of gamma-aminobutyric acid levels and GABA transporter expression. PMID: 24991763
  17. study found that re-expression of GAD67 in neurons following stroke may play a role in aberrant cell cycle activation, consequently being pro-apoptotic PMID: 24983209
  18. Prenatal stress, in addition to heterozygous deletion of Gad1, could specifically disturb the proliferation of neurons destined to be PV-positive GABAergic interneurons. PMID: 24618690
  19. GAD67+/- mice show reduced sociability and decreased intermale aggression, but normal nest building and urine marking behavior, as well as unchanged locomotor activity and anxiety-like behavior. PMID: 24612522
  20. The adoption of the GAD67-GFP mouse model will certainly boost future functional imaging and gene expression analysis of the mouse NEB microenvironment. PMID: 23568330
  21. Sex differences in reproductive genes Gad1, Kiss1 and Gnrh1 are critically disturbed in GABAB1-deficient mice, probably altering the organization and development of neural circuits governing the reproductive axis. PMID: 24080944
  22. In the absence of GAD67, the electrical activity underlying glutamatergic waves shows increased tendency to burst, indicating that GABAergic inputs inhibit the glutamatergic pacemaker, even when GABAergic pacemaker circuitry can itself initiate waves. PMID: 24623764
  23. Orientation selectivity is decreased in mice in which GAD67 and therefore GABA are down-regulated during development. PMID: 23533083
  24. GAD67 expression in dentate gyrus granule cells is a transient marker of late cell differentiation that persists throughout life. PMID: 23565079
  25. Prenatally immune-challenged offspring exhibit reduced glutamic acid decarboxylase 65-kDa (GAD65) and 67-kDa (GAD67) gene expression in the adult medial prefrontal cortex and dorsal hippocampus. PMID: 23876745
  26. We failed to provide proof-of-concept evidence that lower PV and GAT1 expression in schizophrenia are a consequence of lower GAD67 expression PMID: 23103418
  27. Behavioral experiments showed that LV knockdown of GAD67 results in a deficit in the extinction, but not the acquisition or retention, of fear in mice. Diminished GAD67 in the amygdala blunted the anxiolytic-like effect of diazepam PMID: 23149445
  28. This study demonistrated that both protein and mRNA expression of GAD67 in the whole septum was up-regulated in postpartum mice. PMID: 22750123
  29. The mouse lateral striatum concentrates medium-sized projection neurons with high-level expression of GAD1, but not of GAD2, mRNA. PMID: 22332935
  30. activity-driven expression of GAD67 critically controls GABA synthesis and, thus, vesicular filling of the transmitter PMID: 22723692
  31. Anti-idiotypic antibodies specific to GAD65Ab have a protective role in type 1 diabetes PMID: 22384267
  32. reduced GAD67 mRNA expression can induce lower CB1R mRNA expression. PMID: 22036037
  33. At the mRNA level, the expression of GAD67 and of St8SiaII was significantly reduced by chronic stress in the amygdala of adult mice. PMID: 21819983
  34. a GAD67 heterozygous deletion itself enhances vulnerability by many aspects, e.g., maternal stress, maternity, and being in utero. PMID: 21185888
  35. Cleft palate is caused by CNS dysfunction in Gad1 and Viaat knockout mice PMID: 20333300
  36. Down-regulation of GAD67 is associated with autoimmune type 1 diabetes mellitus. PMID: 20414703
  37. Localized expression of Gad1 transcripts in multiple differentiated non-neural tissues of mouse embryos suggests a wider role for GABA signaling in development than was previously appreciated. PMID: 11178105
  38. glucose responsiveness and transcription mediated by PDX-1 PMID: 12150938
  39. No significant GAD67 gene expression differences are detected in either drug-naive or acute ethanol withdrawn mouse brain. PMID: 12691782
  40. GAD67 expression decreases in frontal cortex, parietal cortex, globus pallidus, entopeduncular nucleus and substantia nigra pars reticulata of R6/2 transgenic mice; at 12.5 weeks of age the pathology differs from that in humans with Huntington's disease PMID: 12950446
  41. RELN and GAD67 promoters express an increased recruitment of methyl-CpG binding domain proteins. PMID: 16113080
  42. GAD67 is localized in the dermis and is potentially involved in variety of skin activities. PMID: 17113713
  43. histone hyperacetylation induces demethylation of reelin and 67-kDa glutamic acid decarboxylase promoters PMID: 17360583
  44. lack of gad67 impairs the function of the respiratory network in mouse fetuses and the impairment progresses with fetal age PMID: 17418495
  45. Temporal migration of gonadotrophin-releasing hormone-1 neurones is modified in GAD67 knockout mice. PMID: 18081557
  46. Mice expressing green fluorescent protein (GFP)under the control of GAD67 promoter, exhibit numerous GFP-positive neurons in the reticular formation during rapid eye movement sleep. PMID: 18215233
  47. In GAD67 deficient mouse, there was reduced level of GABA (16-44%)and inhibitory synaptic transmission to Purkinje cells was seriously impaired. PMID: 18384748
  48. the GABA shunt of SUR1(-/-) islets is blocked by about 75% and showed that this defect is due to decreased glutamate decarboxylase synthesis PMID: 18445600
  49. Glutamate-induced excitotoxic stimulation of NMDAR in cultured cortical neurons leads to altered GAD protein levels from GABAergic neurons through intracellular calcium increase and protease activation. PMID: 18599042
  50. Deletion of GAD67 in Dopamine Receptor-1 Expressing Cells Causes Specific Motor Deficits. PMID: 18615733

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Protein Families Group II decarboxylase family
Database Links

KEGG: mmu:14415

STRING: 10090.ENSMUSP00000092539

UniGene: Mm.272120

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