Mouse Glutaredoxin-related protein 5, mitochondrial(GLRX5) ELISA kit

Code CSB-EL009524MO
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details

Target Name
glutaredoxin 5
Alternative Names
Glrx5Glutaredoxin-related protein 5 ELISA Kit; mitochondrial ELISA Kit; Monothiol glutaredoxin-5 ELISA Kit
Abbreviation
GLRX5
Uniprot No.
Species
Mus musculus (Mouse)
Sample Types
serum, plasma, tissue homogenates, cell lysates
Detection Range
31.25 pg/mL-2000 pg/mL
Sensitivity
7.8 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Metabolism
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse GLRX5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 94
Range % 90-96
1:2 Average % 96
Range % 92-100
1:4 Average % 84
Range % 80-88
1:8 Average % 93
Range % 88-97
Recovery
The recovery of mouse GLRX5 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 98 94-102
EDTA plasma (n=4) 106 102-113
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
2000 2.639 2.524 2.582 2.431
1000 2.172 2.068 2.120 1.969
500 1.593 1.523 1.558 1.407
250 0.925 0.892 0.909 0.758
125 0.587 0.571 0.579 0.428
62.5 0.368 0.346 0.357 0.206
31.25 0.256 0.247 0.252 0.101
0 0.149 0.153 0.151  
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Mouse GLRX5 ELISA Kit was designed for the quantitative measurement of Mouse GLRX5 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 31.25 pg/mL-2000 pg/mL and the sensitivity is 7.8 pg/mL.

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 Q&A
Q:

Mouse GLRX5 Elisa Kit
1. From which species is the primary antibody? Is it monoclonal or polyclonal? Is he also separated from that
Kit available?
2. Is the primary antibody directed against the whole protein GRX1 or only against a specific one?
Amino acid sequence?
3. Which protein is in the standards against which the antibody is directed?

A:
Thanks for your inquiry! CSB-EL009524MO is designed for full-length and taking a reference from this link:https://www.uniprot.org/uniprot/Q80Y14
Here is the detailed information:
Coated anti: Rat monoclonal antibody
Detect anti: Goat polyclonal antibody
Standard: NS0-derived recombinant protein

Target Background

Function
(From Uniprot)
Monothiol glutaredoxin involved in mitochondrial iron-sulfur (Fe/S) cluster transfer. Receives 2Fe/2S clusters from scaffold protein ISCU and mediates their transfer to apoproteins, to the 4Fe/FS cluster biosynthesis machinery, or export from mitochondrion. Required for normal regulation of hemoglobin synthesis by the iron-sulfur protein ACO1.
Gene References into Functions
  1. Study reports a decrease of Grx expression levels in pancreatic islets of diabetic mice which was accompanied by declining insulin secretion, increase of reactive oxygen species (ROS) production level, and cell cycle alterations. These data demonstrate the essential role of the Grx system for the beta-cell during metabolic stress which may provide a new target for diabetes mellitus type 2 treatment. PMID: 28542222
  2. Grx5 is an important determinant of osteoblast apoptosis and acts via a molecular pathway that involves regulation of ROS production, cardiolipin oxidation, caspase activity, Fe-S cluster formation, and MnSOD activity. PMID: 19442627
Subcellular Location
Mitochondrion matrix.
Protein Families
Glutaredoxin family, Monothiol subfamily
Tissue Specificity
Detected in bone, liver, muscle and kidney.
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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