Mouse Hepatocyte growth factor activator(HGFAC) ELISA kit

Instructions
Code CSB-EL010328MO
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name HGF activator
Alternative Names Hgfac ELISA Kit; Hepatocyte growth factor activator ELISA Kit; HGF activator ELISA Kit; HGFA ELISA Kit; EC 3.4.21.-) [Cleaved into: Hepatocyte growth factor activator short chain; Hepatocyte growth factor activator long chain] ELISA Kit
Abbreviation HGFAC
Uniprot No. Q9R098
Species Mus musculus (Mouse)
Sample Types serum, plasma, cell culture supernates, tissue homogenates
Detection Range 31.25 pg/mL-2000 pg/mL
Sensitivity 7.8 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cardiovascular
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse HGFAC in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 104
Range % 98-109
1:2 Average % 97
Range % 90-101
1:4 Average % 92
Range % 86-95
1:8 Average % 97
Range % 93-102
Recovery
The recovery of mouse HGFAC spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 94 87-98
EDTA plasma (n=4) 98 92-103
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
2000 2.580 2.469 2.525 2.355
1000 1.826 1.902 1.864 1.694
500 1.203 1.183 1.193 1.023
250 0.682 0.664 0.673 0.503
125 0.399 0.385 0.392 0.222
62.5 0.277 0.284 0.281 0.111
31.25 0.203 0.209 0.206 0.036
0 0.169 0.171 0.170  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 7-14 working days

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Target Data

Function Activates hepatocyte growth factor (HGF) by converting it from a single chain to a heterodimeric form.
Gene References into Functions
  1. HGFA is required for repair of injured intestinal mucosa but is not essential for normal development during embryogenesis or after birth. PMID: 15521012
Subcellular Location Secreted
Protein Families Peptidase S1 family
Database Links

KEGG: mmu:54426

STRING: 10090.ENSMUSP00000030985

UniGene: Mm.27369

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