Mouse Hepatocyte growth factor activator(HGFAC) ELISA kit

Code CSB-EL010328MO
See More Details
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. Q9R098
Lead Time 7-14 working days
Abbreviation HGFAC
Target Name HGF activator
Alias RP11-529E10.2, HGFA, MGC138395, MGC138397,
Species Mus musculus (Mouse)
Sample Types serum, plasma, cell culture supernates, tissue homogenates
Detection Range 31.25 pg/mL-2000 pg/mL
Sensitivity 7.8 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cardiovascular
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details This protein belongs to peptidase family S1. It is first synthesized as an inactive single-chain precursor before being activated to a heterodimeric form by endoproteolytic processing. It acts as serine protease that converts hepatocyte growth factor to the active form.
HGNC 4894
RGD 70909
MGI 1859281
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse HGFAC in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 104
Range % 98-109
1:2 Average % 97
Range % 90-101
1:4 Average % 92
Range % 86-95
1:8 Average % 97
Range % 93-102
Recovery
The recovery of mouse HGFAC spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 94 87-98
EDTA plasma (n=4) 98 92-103
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
2000 2.580 2.469 2.525 2.355
1000 1.826 1.902 1.864 1.694
500 1.203 1.183 1.193 1.023
250 0.682 0.664 0.673 0.503
125 0.399 0.385 0.392 0.222
62.5 0.277 0.284 0.281 0.111
31.25 0.203 0.209 0.206 0.036
0 0.169 0.171 0.170  
References
Still Have Questions? Leave a Message or Start an on-line Chat
Function Activates hepatocyte growth factor (HGF) by converting it from a single chain to a heterodimeric form.
Subcellular Location Secreted
Protein Families Peptidase S1 family
Database Links

KEGG: mmu:54426

STRING: 10090.ENSMUSP00000030985

UniGene: Mm.27369

Related Products

Hgfac Proteins

Hgfac Proteins for Mus musculus (Mouse)

Most popular with customers

Newsletters

Get all the latest information on Events, Sales and Offers. Sign up for newsletter today.

Copyright © 2007-2018 www.cusabio.com CUSABIO TECHNOLOGY LLC All Rights Reserved.