Mouse Hepatocyte growth factor activator(HGFAC) ELISA kit

Code CSB-EL010328MO
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application

Product Details

Target Name HGF activator
Alternative Names RP11-529E10.2, HGFA, MGC138395, MGC138397,
Abbreviation HGFAC
Uniprot No. Q9R098
Species Mus musculus (Mouse)
Sample Types serum, plasma, cell culture supernates, tissue homogenates
Detection Range 31.25 pg/mL-2000 pg/mL
Sensitivity 7.8 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cardiovascular
Assay Principle quantitative
Measurement Sandwich
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
To assess the linearity of the assay, samples were spiked with high concentrations of mouse HGFAC in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 104
Range % 98-109
1:2 Average % 97
Range % 90-101
1:4 Average % 92
Range % 86-95
1:8 Average % 97
Range % 93-102
The recovery of mouse HGFAC spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 94 87-98
EDTA plasma (n=4) 98 92-103
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
2000 2.580 2.469 2.525 2.355
1000 1.826 1.902 1.864 1.694
500 1.203 1.183 1.193 1.023
250 0.682 0.664 0.673 0.503
125 0.399 0.385 0.392 0.222
62.5 0.277 0.284 0.281 0.111
31.25 0.203 0.209 0.206 0.036
0 0.169 0.171 0.170  
and FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 7-14 working days
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Target Data

Function Activates hepatocyte growth factor (HGF) by converting it from a single chain to a heterodimeric form.
Subcellular Location Secreted
Protein Families Peptidase S1 family
Database Links

KEGG: mmu:54426

STRING: 10090.ENSMUSP00000030985

UniGene: Mm.27369

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