Mouse High density lipoprotein cholesterol, HDL-C ELISA kit

Code CSB-E12874m
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name High density lipoprotein cholesterol, HDL-C
Abbreviation HDL-C
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates
Detection Range 7.8 ng/mL-500 ng/mL
Sensitivity 1.95 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Others
Assay Principle quantitative
Measurement Sandwich
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
To assess the linearity of the assay, samples were spiked with high concentrations of mouse HDL-C in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
1:1000Average %88
Range %84-92
1:2000Average %91
Range %85-97
1:4000Average %99
Range %95-104
1:8000Average %92
Range %88-96
The recovery of mouse HDL-C spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9690-102
EDTA plasma (n=4)9791-104
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
5002.509 2.598 2.554 2.440
2502.045 2.068 2.057 1.943
1251.401 1.457 1.429 1.315
62.50.977 0.999 0.988 0.874
31.20.572 0.598 0.585 0.471
15.60.315 0.338 0.327 0.213
7.80.256 0.267 0.262 0.148
00.113 0.115 0.114  
and FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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