Mouse Histone deacetylase complex subunit SAP130(SAP130) ELISA kit

Code CSB-EL020694MO
Size 96T,5×96T,10×96T
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Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details

Target Name
Sin3A-associated protein, 130kDa
Alternative Names
Sap130 ELISA Kit; Histone deacetylase complex subunit SAP130 ELISA Kit; 130 kDa Sin3-associated polypeptide ELISA Kit; Sin3-associated polypeptide p130 ELISA Kit
Abbreviation
SAP130
Uniprot No.
Species
Mus musculus (Mouse)
Sample Types
serum, plasma, tissue homogenates, cell lysates
Detection Range
46.88 pg/mL-3000 pg/mL
Sensitivity
11.72 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Epigenetics and Nuclear Signaling
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse SAP130 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %92
Range %85-99
1:2Average %98
Range %93-102
1:4Average %93
Range %89-98
1:8Average %91
Range %87-97
Recovery
The recovery of mouse SAP130 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9287-97
EDTA plasma (n=4)9493-106
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
30002.622 2.535 2.579 2.430
15002.117 2.154 2.136 1.987
7501.402 1.520 1.461 1.312
3750.736 0.780 0.758 0.609
187.50.478 0.495 0.487 0.338
93.750.311 0.316 0.314 0.165
46.880.245 0.240 0.243 0.094
00.153 0.144 0.149
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Mouse SAP130 ELISA Kit was designed for the quantitative measurement of Mouse SAP130 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 46.88 pg/mL-3000 pg/mL and the sensitivity is 11.72 pg/mL.

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Target Background

Function
(From Uniprot)
Acts as a transcriptional repressor. May function in the assembly and/or enzymatic activity of the mSin3A corepressor complex or in mediating interactions between the complex and other regulatory complexes.
Subcellular Location
Nucleus.
Protein Families
SAP130 family
Database Links
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