Mouse Hydroxyacid oxidase 1(HAO1) ELISA kit

Code CSB-EL010127MO
Size 96T,5×96T,10×96T
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Product Details

Target Name
hydroxyacid oxidase (glycolate oxidase) 1
Alternative Names
Hao1 ELISA Kit; Gox1 ELISA Kit; Hao-1 ELISA Kit; Hydroxyacid oxidase 1 ELISA Kit; HAOX1 ELISA Kit; EC 1.1.3.15 ELISA Kit; Glycolate oxidase ELISA Kit; GOX ELISA Kit
Abbreviation
HAO1
Uniprot No.
Species
Mus musculus (Mouse)
Sample Types
serum, plasma, tissue homogenates, cell lysates
Detection Range
6.25 pg/mL-400 pg/mL
Sensitivity
1.56 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Cardiovascular
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse HAO1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %94
Range %83-107
1:2Average %95
Range %89-103
1:4Average %92
Range %85-105
1:8Average %91
Range %87-99
Recovery
The recovery of mouse HAO1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9892-103
EDTA plasma (n=4)9690-104
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
4002.413 2.347 2.380 2.198
2001.603 1.589 1.596 1.414
1000.902 0.865 0.884 0.702
500.549 0.527 0.538 0.356
250.388 0.363 0.376 0.194
12.50.282 0.272 0.277 0.095
6.250.253 0.237 0.245 0.063
00.180 0.183 0.182
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Mouse HAO1 ELISA Kit was designed for the quantitative measurement of Mouse HAO1 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 6.25 pg/mL-400 pg/mL and the sensitivity is 1.56 pg/mL.

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Target Background

Function
(From Uniprot)
Has 2-hydroxyacid oxidase activity. Most active on the 2-carbon substrate glycolate, but is also active on 2-hydroxy fatty acids, with high activity towards 2-hydroxy palmitate and 2-hydroxy octanoate.
Subcellular Location
Peroxisome.
Protein Families
FMN-dependent alpha-hydroxy acid dehydrogenase family
Tissue Specificity
Liver.
Database Links
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