Mouse Indian Hedgehog (IHH) ELISA kit

Instructions
Code CSB-E16517m
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name Indian Hedgehog (IHH)
Alternative Names IhhIndian hedgehog protein ELISA Kit; IHH ELISA Kit; HHG-2) [Cleaved into: Indian hedgehog protein N-product; Indian hedgehog protein C-product] ELISA Kit
Abbreviation IHH
Uniprot No. P97812
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates
Detection Range 3.12 pg/mL-200 pg/mL
Sensitivity 0.78 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Stem Cells
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse IHH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 86
Range % 82-91
1:2 Average % 101
Range % 98-105
1:4 Average % 87
Range % 84-91
1:8 Average % 100
Range % 95-105
Recovery
The recovery of mouse IHH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 89 85-94
EDTA plasma (n=4) 107 103-110
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
200 1.785 1.831 1.808 1.687
100 1.181 1.201 1.191 1.070
50 0.737 0.800 0.769 0.648
25 0.455 0.435 0.445 0.324
12.5 0.301 0.312 0.307 0.186
6.25 0.208 0.204 0.206 0.085
3.12 0.167 0.161 0.164 0.043
0 0.122 0.120 0.121  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Data

Function Intercellular signal essential for a variety of patterning events during development. Binds to the patched (PTC) receptor, which functions in association with smoothened (SMO), to activate the transcription of target genes. Implicated in endochondral ossification
Gene References into Functions
  1. Noggin null mutants show increased Indian hedgehog (Ihh) expression within cartilage condensations that leads to aberrant extension of IHH downstream signaling into the interdigital mesenchyme. A converse phenotype with increased apoptosis and reduced cell proliferation was found in the interdigital mesenchyme of Ihh mutant embryos, suggesting IHH's unrecognized physiological role for IHH in interdigital webbing biology. PMID: 29771958
  2. This work reveals the molecular function of Hedgehog signaling in extrinsic sheath tissues for tendon repair. PMID: 29244023
  3. melatonin not only elevated progesterone (P) secretion, but also upregulated expressions of StAR and Cyp11a1 and also had an increased Ihh expression in endometrium. PMID: 28749439
  4. loss of Spop, but not Spopl, disrupts chondrocyte hypertrophy and osteoblast differentiation in the mouse, suggesting the requirement for Spop-mediated protein degradation in mouse skeletal development; overexpressed Spop targets both Gli3FL and Gli3R for ubiquitination and degradation and Spop is an important positive regulator of Ihh signaling and skeletal development PMID: 27930311
  5. Ihh is regulated by at least 9 enhancers with individual tissue specificities in the digit anlagen, growth plates, skull sutures and fingertips. Consecutive deletions, resulting in growth defects of the skull and long bones, showed that these enhancers function in an additive manner. Duplications caused dose-dependent upregulation and misexpression of Ihh, abnormal phalanges, fusion of sutures and syndactyly. PMID: 28846100
  6. GPC6 stimulates Hh signaling by binding to Hh and Ptc1 at the cilium and increasing the interaction of the receptor and ligand to promote the growth of developing long bones. PMID: 28696225
  7. An accelerated hypertrophic differentiation caused by a disturbed Ihh-PTHrP signaling pathway may lead to a higher bone mineral density in the vertebral bodies of newborn Col IX -/- mice and, as a result, to the early onset of disc degeneration. PMID: 27666725
  8. findings thus demonstrate that augmented Ihh signaling is detrimental to craniofacial development, and that finely tuned Ihh signaling is critical for temporomandibular joint formation. PMID: 26553654
  9. Ihh has an important role in regulating limb mesenchymal cell differentiation PMID: 26094741
  10. Ihh and PTH1R signaling in limb mesenchyme are both essential to regulate proper development of digit structures, although they appear to use different mechanisms. PMID: 26620087
  11. The Annexin a2 Promotes Development in Arthritis through Neovascularization by Amplification Hedgehog Pathway. PMID: 26963384
  12. Ihh expression was downregulated in femur epiphyses of Hand1-overexpressing mice. Hand1 downregulated Ihh gene expression in vitro by inhibiting Runx2 transactivation of the Ihh proximal promoter. PMID: 26918743
  13. In organogenesis of the ovary, production of Dhh/Ihh in granulosa cells requires growth differentiation factor 9 from the oocyte. PMID: 25917826
  14. increased expression in cell bodies of cutaneous sensory nerves of dorsal root ganglia as compared with skin epithelia PMID: 25842978
  15. C/EBPbeta and RUNX2 cooperatively stimulate expression of Ihh through direct interactions with a C/EBPbeta binding element, which further promotes hypertrophic differentiation of chondrocytes during the chondrocyte differentiation process. PMID: 25105964
  16. findings support the protective role of Ihh deletion in surgically induced osteoarthritis PMID: 24428864
  17. Thyroid hormone regulates the secondary ossification center initiation and progression via differentiating chondrocytes into bone matrix-producing osteoblasts by stimulating Ihh and Osx expression in chondrocytes. PMID: 24753031
  18. Apc mutant epithelial cells secrete IHH to maintain an intestinal stromal phenotype that is required for adenoma development in mice. PMID: 25307863
  19. Cbfbeta functions in upregulating Ihh expression to promoter chondrocyte proliferation and osteoblast differentiation, and inhibiting PPR expression to enhance chondrocyte differentiation. PMID: 24821091
  20. Indian Hedgehog, a critical modulator in osteoarthritis, could be a potential therapeutic target for attenuating cartilage degeneration disease. PMID: 24844414
  21. Data indicate Indian hedgehog (Ihh) induces collagen type X COL10A1 expression and promotes its transcription through Runx2/Smad1 interactions on a specific promoter region. PMID: 25028519
  22. Increased Ihh is associated with the severity of osteoarthritis cartilage damage. PMID: 24786088
  23. Ihh signaling mediates gastrin-induced proliferation of epithelial cells in stomachs of adult mice. PMID: 24859162
  24. Sirt6 regulates postnatal growth plate differentiation and proliferation via Ihh signaling. PMID: 24149372
  25. Data indicate that the centrosomal protein DZIP1 interacts with GLI3 transcription factor in the cytoplasm and regulates Hedgehog signaling and ciliogenesis. PMID: 23955340
  26. Data suggest that GPC3 down-regulates hepatocyte proliferation by binding to hedgehog (HH) and down-regulating the HH signaling pathway and binding with CD81, thus making it unavailable to bind to Hhex and causing its nuclear translocation. PMID: 23665349
  27. Ihh signaling plays an important role in chondrocyte proliferation and differentiation via interactions with Runx2 and Runx3. PMID: 23383321
  28. GABA(B)R1 positively regulates chondrogenesis through a mechanism relevant to the acceleration of nuclear translocation of ATF4 for Ihh expression in chondrocytes PMID: 22879594
  29. The data indicated that Atf4 in chondrocytes is required for normal Ihh expression and for its paracrine effect on osteoblast differentiation. PMID: 22190639
  30. Indian hedgehog requires additional effectors besides Runx2 to induce osteoblast differentiation. PMID: 22155527
  31. study defines Ihh as an important positive regulator of cranial bone ossification PMID: 21557453
  32. IGF-1R in chondrocytes controls cell growth, survival, and differentiation in embryonic and postnatal growth plates in part by suppression of Pthrp expression. PMID: 21312270
  33. Loss of muscle mass in Ihh null mouse embryos is accompanied by a dramatic increase in myoblast apoptosis by a loss of p21 protein PMID: 21683695
  34. Ihh signaling is essential for symphyseal cartilage development and anterior mandibular growth PMID: 21297010
  35. The hedgehog pathway is activated during palatal fusion, and deletion of Indian hedgehog leads to diminished ossification of the secondary hard palate. PMID: 21364421
  36. Loss of Ihh initiates several events that are characteristic of an intestinal wound repair response. Prolonged loss resulted in progressive inflammation, mucosal damage, and the development of intestinal fibrosis. PMID: 20682322
  37. Ihh regulates intestinal stem cell self-renewal and differentiation. Intestinal epithelial Ihh signals to mesenchymal cells to regulate formation and proliferation of mesenchymal cells, which in turn affect epithelial proliferation and differentiation. PMID: 20542037
  38. Indian hedgehog and WNT/beta-catenin signaling, inhibited by receptor tyrosine kinase-like orphan receptor 2 (ROR2) in distal limb mesenchyme, are acting upstream of BMP signaling in the phalanx-forming region PMID: 20660756
  39. Ihh regulates embryo implantation by having an impact on the cell cycle, EGF signaling, and E2 signaling. PMID: 20056671
  40. Hh proteins act as paracrine mitogens to promote the expansion of adjacent mesenchymal progenitors, including those of the smooth muscle compartment. PMID: 20430747
  41. Ihh has both PTHrP-dependent and -independent functions during postnatal endochondral bone development. PMID: 19761883
  42. Sostdc1, Penk1 and Igfbp5 were identified as possible new IHH signaling targets that may contribute to the molecular mechanism for BDA1. PMID: 20024692
  43. continuous Ihh action is required for completion of post-natal temporomandibular joint growth and organization PMID: 20200412
  44. Hedgehog signaling may be important stimulus for renal cyst formation. PMID: 20007344
  45. Atf4 as a novel transcriptional activator of Indian hedgehog in chondrocytes that paces longitudinal bone growth PMID: 19906842
  46. Gli2 and Gli3 collectively mediate all major aspects of Ihh function during endochondral skeletal development PMID: 19906844
  47. Indian hedgehog as a progesterone-responsive factor mediating epithelial-mesenchymal interactions in the mouse uterus. PMID: 11977981
  48. Data suggest that Grg5 modulates the activities of transcription factors in vivo to affect Ihh expression and the function of long bone growth plates. PMID: 11984876
  49. Indian hedgehog control differentiation of growth plate chondrocytes at multiple steps. PMID: 12050144
  50. neither Shh nor Ihh is required for mammary gland morphogenesis and functional differentiation PMID: 12217324

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Subcellular Location Indian hedgehog protein N-product: Cell membrane, Lipid-anchor, Extracellular side, Note=The N-terminal peptide remains associated with the cell surface, SUBCELLULAR LOCATION: Indian hedgehog protein C-product: Secreted, extracellular space
Protein Families Hedgehog family
Tissue Specificity In the embryo, detected in the developing gut, the growth zone of cartilage of developing long bones, epithelium and urogenital sinus. In the adult kidney, found in proximal convoluted and proximal straight tubule.
Database Links

KEGG: mmu:16147

STRING: 10090.ENSMUSP00000128056

UniGene: Mm.439736

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