Mouse Indoleamine 2,3-dioxygenase 1(IDO1) ELISA kit

Instructions
Code CSB-EL010996MO
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name indoleamine 2,3-dioxygenase 1
Alternative Names Ido1 ELISA Kit; Ido ELISA Kit; Indo ELISA Kit; Indoleamine 2,3-dioxygenase 1 ELISA Kit; IDO-1 ELISA Kit; EC 1.13.11.52 ELISA Kit; Indoleamine-pyrrole 2,3-dioxygenase ELISA Kit
Abbreviation IDO1
Uniprot No. P28776
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates
Detection Range 62.5 pg/mL-4000 pg/mL
Sensitivity 15.6 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Metabolism
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse IDO1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 90  
Range % 86-94  
1:2 Average % 98  
Range % 92-101  
1:4 Average % 90  
Range % 87-95  
1:8 Average % 91  
Range % 85-97  
Recovery
The recovery of mouse IDO1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 93 89-98  
EDTA plasma (n=4) 99 95-105  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
4000 2.878 3.112 2.995 2.869  
2000 2.217 2.374 2.296 2.170  
1000 1.475 1.535 1.505 1.379  
500 0.933 0.953 0.943 0.817  
250 0.571 0.618 0.595 0.469  
125 0.372 0.401 0.387 0.261  
62.5 0.261 0.260 0.261 0.135  
0 0.121 0.130 0.126    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 5-7 working days

Target Data

Function Catalyzes the first and rate limiting step of the catabolism of the essential amino acid tryptophan along the kynurenine pathway. Involved in the peripheral immune tolerance, contributing to maintain homeostasis by preventing autoimmunity or immunopathology that would result from uncontrolled and overreacting immune responses. Tryptophan shortage inhibits T lymphocytes division and accumulation of tryptophan catabolites induces T-cell apoptosis and differentiation of regulatory T-cells. Acts as a suppressor of anti-tumor immunity
Gene References into Functions
  1. Influenza infection of NIH-3T3 cells elevates the expression of indoleamine 2,3 dioxygenase (IDO). Inhibition against IDO followed by infection increases the level of viral RNA and reduces the upregulation of 3-hydroxyanthranilate 3,4-dioxygenase driven by virus. Induction of IDO appears to contribute to limiting replication of the WSN/33 strain of influenza A virus in murine NIH-3T3 cells. PMID: 28402179
  2. Study showed that the knockout of IDO prevented vascular smooth muscle cells apoptosis in AngII -treated Ldlr-/- mice fed with HFD, suggesting a detrimental role of IDO in abdominal aortic aneurysm formation. PMID: 29494675
  3. The KYNurenine pathway of IDO1-mediated Tryptophan metabolism plays a critical role in depressive symptoms associated with IFN-alpha therapy. PMID: 27436416
  4. IDO is a critical regulator of acute pulmonary inflammation . PMID: 28673995
  5. Data suggest that Indoleamine 2,3-dioxygenase 1 (IDO1) appears to be a potential hallmark of liver lesions, and its deficiency protects mice from CCl4-induced fibrosis mediated by Th17 cells down-regulation and tryptophan 2,3-dioxygenase (TDO) compensatory increase. PMID: 28465467
  6. Indoleamine 2,3-dioxygenase regulates anti-tumor immunity in lung cancer by metabolic reprogramming of immune cells in the tumor microenvironment PMID: 27705910
  7. Findings suggest non-redundant neurophysiological roles for indoleamine 2,3-dioxygenase 1, indoleamine 2,3-dioxygenase 2 and tryptophan 2,3-dioxygenase in modulating brain activities and metabolism. PMID: 27316339
  8. These results show IDO is upregulated with RSV infection and this upregulation likely participates with IFN-gamma in inhibition of virus replication and suppression of some host cell responses to infection. PMID: 28963880
  9. Lipopolysaccharide (LPS) stimulation increased the expression and activity of the immunoregulatory enzyme IDO1 in hepatic stellate cells (HSCs), and LPS/HSCs stimulated aryl hydrocarbon receptor (AhR) signaling in cocultured regulatory T cells. PMID: 27581538
  10. this study shows that the presence of IFN-alpha at antigen sensitization activates an IDO1/TGF-beta-dependent anti-inflammatory program that upon antigenic rechallenge prevents inflammation via plasmacytoid dendritic cells PMID: 27647832
  11. The deficiency of indoleamine 2,3-dioxygenase aggravates the carbon tetrachloride-induced liver fibrosis in mice. PMID: 27598994
  12. Across strains, networks depicted a predominance of genes under-expressed in microglia relative to macrophages that may be a precursor for the different response of both cell types to challenges. The detected transcriptome differences enhance the understanding of the role of IDO1 in the microglia transcriptome under unchallenged conditions. PMID: 27314674
  13. Data show that indoleamine 23-dioxygenase 1 (IDO-1) inhibitors 1-methyl-D-tryptophan was able to alleviate most of the behavioural changes resulting from unpredictable chronic mild stress (UCMS) exposure. PMID: 27828964
  14. IDO did not play a pivotal role in the suppression of allergic airway inflammation through adipose-derived stem cells, suggesting that it is not the major regulator responsible for suppressing allergic airway inflammation. PMID: 27812173
  15. Aortic Plasmacytoid dendritic cells expressed CCR9 and indoleamine 2,3-dioxygenase 1 (IDO-1), an enzyme involved in driving the generation of regulatory T cells (Tregs). PMID: 27166946
  16. Indoleamine-2,3-dioxygenase (IDO) production by Plasmacytoid dendritic cells (pDCs)is necessary to confer suppressive function to T-Cells, Regulatory (Tregs) in experimental autoimmune encephalomyelitis (EAE). PMID: 27470005
  17. our findings support the hypothesis elevated IDO activity in non-CNS due to virus infections causes pain hypersensitivity PMID: 27168185
  18. this study shows that IDO overexpression in dendritic cells attenuates acute allograft rejection PMID: 27107370
  19. This insight into IDO1's involvement in pro-tumorigenic inflammatory neovascularization may have important ramifications for IDO1 inhibitor development, not only in cancer where clinical trials are currently ongoing, but in other disease indications associated with neovascularization as well. PMID: 27889479
  20. Inhibition of IDO activity ameliorated Japanese encephalitis via enhancement of antiviral IFN-I/II innate and adaptive T-cell responses and increased central nervous system infiltration of peripheral leukocytes. PMID: 27090635
  21. Results suggest that IDO expression is implicated in immunosuppression and tumor progression in glioma cells; combining IDO inhibition with standard TMZ treatment could be an encouraging therapeutic strategy for patients with malignant glioma PMID: 26636389
  22. Data show that the expression of indoleamine 2, 3-dioxygenase 1 (IDO) was decreased after tumor cells were infected with Salmonella. PMID: 26517244
  23. Severity of sodium dodecyl sulfate-induced colitis is reduced in Ido1-deficient mice with down-regulation of TLR-MyD88-NF-kB transcriptional networks. PMID: 26610689
  24. Data implicate indoleamine 2,3-dioxygenase-dependent neurotoxic kynurenine metabolism as a pathogenic factor for cognitive dysfunction in inflammation-induced depressive disorders and a potential novel target for the treatment of these disorders. PMID: 26130057
  25. IDO1 deficiency does not affect inflammation in Systemic Juvenile Idiopathic Arthritis, Secondary Hemophagocytic Lymphohistiocytosis and a T cell-triggered cytokine release model. PMID: 26914138
  26. Increased expression of IDO in liver cell adenomas compared to the surrounding normal tissue may create a microenvironment that promotes the progression of HCC by suppressing the proliferation of cytotoxic T lymphocytes and enhancing Tregs. PMID: 26727596
  27. Chimeric vaccine stimulation of human dendritic cell IDO1 occurs via the non-canonical NF-kappaB pathway. PMID: 26881431
  28. Absence of Ido1 protects against atherosclerosis through increase of Il10. PMID: 26235422
  29. Indoleamine 2,3-Dioxygenase Is Involved in the Inflammation Response of Corneal Epithelial Cells to Aspergillus fumigatus Infections PMID: 26361229
  30. the role of IFN-lambda in IDO regulation was investigated after influenza infection of respiratory epithelial cells. PMID: 25756191
  31. TNF-alpha mediates stress-induced depression by upregulating indoleamine 2,3-dioxygenase in a mouse model of unpredictable chronic mild stress. PMID: 26083579
  32. Experimental hemophilic mouse models with or without functional IDO1 revealed that tryptophan metabolites, which result from IDO1 activity, prevent generation of anti-FVIII antibodies. PMID: 26426076
  33. Data indicate indoleamine 23-dioxygenase 1 IDO1 induction in B cells as a negative regulatory mechanism of the T Cell-independent antigens (TI) humoral immune response. PMID: 26216892
  34. IFN-gamma coordinately induces IDO1 and a tryptophan-selective transporter in human colonic epithelial cells and mouse dendritic cells with a positive feedback mechanism via kynurenine-AhR signaling. PMID: 25450809
  35. The data showed that there is not significant effect of IDO1 or TDO2 on mortality in pneumococcal meningitis. PMID: 24844751
  36. dendritic cell-based immune response mediated by interferon-gamma-induced IDO expression via GSK-3beta activity not only regulates CD8(+) T-cell proliferation and cytotoxic T lymphocyte activity but also modulates OVA-pulsed DC vaccination against EG7 thymoma PMID: 25814664
  37. Data suggest that transferred TGF-beta-induced regulatory T cells (iTregs) could induce tolerogenic splenic dendritic cells and these cells could effectively dampen collagen-induced arthritis in an indoleamine 2,3-dioxygenase (IDO)-dependent manner. PMID: 25405209
  38. 20 weeks of Western diet altered LPS-induced depressive-like behavior compared to LPS-treated lean mice and exacerbated hippocampal and hypothalamic proinflammatory cytokine expression and brain IDO activation. PMID: 24681251
  39. B7-2 costimulation and intracellular indoleamine 2,3-dioxygenase expression is reduced in umbilical cord blood as compared to adult peripheral blood. PMID: 24930629
  40. These data indicate that activation of brain IDO1 is sufficient to induce depression-like behaviors of mice in response to central LPS. PMID: 23866724
  41. IDO2 is critical for IDO1-mediated T-cell regulation and exerts a non-redundant function in inflammation. PMID: 24402311
  42. Inhibitors were used to clarify the role of IDO in graft-vs-tumor reactions after allogeneic stem cell transplantation plus donor leukocyte infusion. IDO1 expression in tumor tissues and TDLN, but not spleen, was increased in the mice receiving DLI. PMID: 24971697
  43. The role of IDO during initial host response to influenza infection was studied by using a specific inhibitor. IDO inhibition enhanced lung proinflammatory cytokine gene and protein expression at 24 and 48 h post influenza virus infection. PMID: 24799604
  44. engagement of CD80/86 by CTLA-4 induced activation of the enzyme indoleamine 2,3-dioxygenase (IDO) in osteoclast precursors, which degraded tryptophan and promoted apoptosis PMID: 24807557
  45. The study indicates that IDO1 is spatiotemporally expressed in activated microglia during acute viral encephalitis by encephalomyocarditis virus. PMID: 24530381
  46. Systemic primary and recall T-cell CD8 responses to viral antigens are controlled by IDO. PMID: 24587363
  47. tumor-derived IDO promotes the peritoneal dissemination of ovarian cancer through creating an immunotolerogenic environment within the peritoneal cavity PMID: 24826982
  48. Induction of hepatitis B virus surface antigen-specific cytotoxic T lymphocytes can be up-regulated by the inhibition of indoleamine 2, 3-dioxygenase activity. PMID: 24580128
  49. Data indicate that the frequency and absolute number of Treg cells increased in indoleamine 2,3-dioxygenase (IDO) expressing fibroblast environment PMID: 23891282
  50. we address this issue with the development of IDO1 monoclonal antibody 4B7 which specifically recognizes the murine enzyme in tissue sections, offering a reliable tool for immunohistology in preclinical disease models. PMID: 24123235
  51. Up-regulated expression of IDO and PD-L1, as well as recruitment of regulatoryT cells, in the tumor microenvironment depended on the presence of CD8(+) T cells. PMID: 23986400
  52. IDO-Knockout mice developed marked pericellular fibrosis in the liver, accumulated hepatic hydroxyproline, and exhibited increased expression levels of hepatic TGF-beta1 mRNA. PMID: 24039933
  53. Deletion of Ido1 and reduced mRNA expression for Ido2 neither affected the concentration of the downstream metabolites of tryptophan nor mRNA expression for downstream genes on the kynurenine pathway in inguinal lymph nodes. PMID: 23417792
  54. After cornea transplantation, sCD83 induced IDO1 promoter activity at late but not at early time periods in dendritic cells. It induces long-term IDO expression via TGF-beta in DCs both in vitro and in vivo. PMID: 23851696
  55. Immunosuppressive role of IDO on macrophage-mediated xeno-rejection. PMID: 23940687
  56. Collectively, these results demonstrate IDO neither plays an essential role, nor is required, in LP-BM5-induced disease progression or LP-BM5 viral load. PMID: 23680027
  57. Ido1 gene knockout animals succeed in preserving the local epididymal immune situation due to the activation of compensatory mechanisms. PMID: 23840489
  58. induction of IDO1 in HPV-infected skin contributes to evasion of host immunity PMID: 23652797
  59. Chronic Ido1 induction in the striatum of YAC128 mice leads to increased neurotoxicity in this Huntington's disease model. PMID: 23994717
  60. our data indicate that IDO1 plays an important immunoregulatory role in the colon. PMID: 23956437
  61. IDO1, which catabolizes tryptophan, promotes colitis-associated tumorigenesis in mice, independent of its ability to limit T-cell-mediated immune surveillance. PMID: 23669411
  62. The CTLA-4 blockade strongly synergizes with IDO inhibitors to mediate rejection of both IDO-expressing and nonexpressing poorly immunogenic tumors. PMID: 23752227
  63. type 2 diabetes upregulates systemic and local ER stress response contributing to renal injury. However, the accompanying upregulations of PD-1 and IDO likely reflect activation of compensatory mechanisms to curtail inflammation and cell injury. PMID: 23219834
  64. Immunohistochemical analysis in Ido1(-/-) mouse epididymis showed that Ido2 protein was extensively upregulated due to the loss of Ido1 expression. PMID: 22895526
  65. When adoptively transferred, spenic IDO-expressing CD11b+ dendritic cells from tolerized animals suppressed the development of arthritis. PMID: 23121975
  66. Inhibition of increased IDO activity maybe involved in the antiparasitic mechanism during Toxoplasma gondii (T. gondii) infection in vivo. PMID: 22609210
  67. IDO-1-deficiency fails to impact on the immune control and the outcome of the infection in the mouse model of tuberculosis. PMID: 22649518
  68. Inhibition of IDO-dioxygenase in centrally induced neuroinflammation under experimental conditions can prevent the development of depressive-like behavior. PMID: 22112548
  69. role in breast tumor cell proliferation, cell cycle regulation, and antiapoptotic signaling PMID: 22654951
  70. Stimulation of IDO expression represents the strategy of Escherichia coli to create local immune privilege at epithelial surfaces, attenuating innate responses to promote colonization and the establishment of infection. PMID: 22474038
  71. Data establish that Tat induces indoleamine 2,3 dioxygenase expression via an IFNgamma-independent mechanism that depends upon activation of p38 MAPK. PMID: 21810259
  72. IDO-1 and IDO-2 play biologically important, contradictory roles during intracellular protozoal infection - facilitating (Toxoplasma gondii) or suppressing (Leishmania major) microbial clearance in a pathogen-specific manner. PMID: 21990421
  73. CTLA4Ig-induced tolerance to murine cardiac allografts is critically dependent on synergistic cross-linked interplay of IDO and Tregs. PMID: 22131334
  74. Echinococcus granulosus recombinant antigen B can induce IDO expression in bone marrow-derived dendritic cells in vitro. PMID: 21826895
  75. Indoleamine 2,3-dioxygenase is a signaling protein in long-term tolerance by dendritic cells. PMID: 21804557
  76. IDO induction did not occur in T cell-deficient mice or in mice with defective B7 or programmed death (PD)-1 costimulatory pathways PMID: 21813777
  77. the ability of IDO-expressing tumor cells to thrive in a tryptophan-depleted microenvironment by expressing a novel, highly tryptophan-specific transporter, which is resistant to inhibition by most other amino acids PMID: 21742973
  78. The authors demonstrate an increase in the rate of colonic epithelial cell turnover after inhibition of IDO in Trichuris muris-infected SCID mice, leading to a significant expulsion of parasite burden. PMID: 21392042
  79. IDO playing a hitherto unsuspected role in sperm quality control in the epididymis involving the ubiquitination of defective spermatozoa and their subsequent removal. PMID: 21189261
  80. Epithelial cell-mediated protection occurred through a TLR3/TRIF-dependent pathway converging on indoleamine 2,3-dioxygenase (IDO) via non-canonical nuclear factor-kappaB activation. PMID: 20835271
  81. Upregulated type I interferon and suppression of murine leukemia virus replication are demonstrated in IDO-deficient mice, suggesting that modulation of the IDO pathway is an effective strategy for treatment of virus infection. PMID: 20693424
  82. Kynurenine, the first breakdown product in the IDO-dependent tryptophan degradation pathway, activates the aryl hydrocarbon receptor (AHR) required for AHR-dependent generation of CD25positive FoxP3positive regulatory T cells. PMID: 20720200
  83. Psychological stress stimulates cytokine-driven IDO1 activation and Trp depletion which seems to have a central role for developing stress-induced immunosuppression and behavioral alteration. PMID: 20689575
  84. mMSC, unlike hMSC, do not display IDO1-mediated suppression of antigen-specific T-cell responses PMID: 19886804
  85. Data establish that activation of the innate immune system in the brain is sufficient to activate indoleamine 2,3 dioxygenase and induce depressive-like behavior in the absence of detectable IFN gamma. PMID: 20678226
  86. Loss of the IDO regulatory tumor suppressor gene Bin1 in transformed skin cells facilitates IDO-mediated immune escape. PMID: 20640572
  87. Analysis of mechanisms by which IDO controls parasite replication revealed that T. cruzi amastigotes were sensitive to L-kynurenine downstream metabolites 3-hydroxykynurenine and 3-hydroxyanthranilic acid, while 3-HK also affected the trypomastigote stage PMID: 20233946
  88. show that IDO-competent cells express the B-lineage commitment factor Pax5 and surface immunoglobulins. PMID: 20498068
  89. IDO1 is a lung-specific innate immune mechanism that controls pulmonary Francisella infections. PMID: 20385761
  90. These findings support the possibility that targeted induction of IDO-1 is an approach deserving further investigation as a therapeutic strategy for diseases of intestinal inflammation. PMID: 20181893
  91. Data desceribe the expressions of indoleamine 2,3-dioxygenase and FasL mRNA in Kupffer cells pretreated with IFN-gamma. PMID: 20128035
  92. IDO-1 is not directly involved in the pathogenesis of cerebral malaria (CM) in C57BL/6 mice, since genetic deficiency in IDO-1 did not confer any protection against CM PMID: 19000912
  93. These results reveal a previously unsuspected role for IFN-gamma responsiveness in nonhematopoietic cells in regulation of immunity to M. tuberculosis and illustrate the role of IDO in the inhibition of Th17 cell responses. PMID: 20064452
  94. IDO-transfected tumor cells and tissue microenvironments with genetically enhanced IDO activity inhibit T cell proliferation and reduce the number of T cells elicited over time. PMID: 11937528
  95. Indoleamine 2,3-dioxygenase contributes to tumor cell evasion of T cell-mediated rejection. PMID: 12209992
  96. Production of maternal IDO plays a role in resolving bacterial infection in the placenta while at the same time maintaining a barrier to T cells whose presence might result in fetal rejection. PMID: 12517946
  97. (IFN-gamma)-activated macrophages induced indoleamine-2,3-dioxygenase, which may regulate NO-synthase induction and macrophage function by controlling availability of tryptophan. PMID: 12525575
  98. Data show that most human tumors constitutively express indoleamine 2,3-dioxygenase (IDO), and that expression of IDO by immunogenic mouse tumor cells prevents their rejection by preimmunized mice. PMID: 14502282
  99. studies indicate the inhibitory role of toll-like receptor ligand-induced IDO in both Th2-mediated and Th1-mediated lung inflammation PMID: 15254594
  100. Data suggest that indoleamine 2,3-dioxygenase-mediated suppression by plasmacytoid dendritic cells in tumor-draining lymph nodes creates a local microenvironment that is potently suppressive of host antitumor T cell responses. PMID: 15254595

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Subcellular Location Cytoplasm, cytosol
Protein Families Indoleamine 2,3-dioxygenase family
Tissue Specificity Highly expressed in epididymis, duodemum, jejunum, ileum, colon and spleen (PubMed:19741271). Highly expressed in epididymis, prostate, duodemum, jejunum, ileum, colon and spleen, not detected in the liver (at protein level) (PubMed:19741271). Expressed i
Database Links

KEGG: mmu:15930

STRING: 10090.ENSMUSP00000033956

UniGene: Mm.392

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