Mouse Indoleamine 2,3-dioxygenase 1(IDO1) ELISA kit

Instructions
Code CSB-EL010996MO
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name indoleamine 2,3-dioxygenase 1
Alternative Names Ido1 ELISA Kit; Ido ELISA Kit; Indo ELISA Kit; Indoleamine 2,3-dioxygenase 1 ELISA Kit; IDO-1 ELISA Kit; EC 1.13.11.52 ELISA Kit; Indoleamine-pyrrole 2,3-dioxygenase ELISA Kit
Abbreviation IDO1
Uniprot No. P28776
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates
Detection Range 62.5 pg/mL-4000 pg/mL
Sensitivity 15.6 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Metabolism
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse IDO1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 90  
Range % 86-94  
1:2 Average % 98  
Range % 92-101  
1:4 Average % 90  
Range % 87-95  
1:8 Average % 91  
Range % 85-97  
Recovery
The recovery of mouse IDO1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 93 89-98  
EDTA plasma (n=4) 99 95-105  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
4000 2.878 3.112 2.995 2.869  
2000 2.217 2.374 2.296 2.170  
1000 1.475 1.535 1.505 1.379  
500 0.933 0.953 0.943 0.817  
250 0.571 0.618 0.595 0.469  
125 0.372 0.401 0.387 0.261  
62.5 0.261 0.260 0.261 0.135  
0 0.121 0.130 0.126    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 5-7 working days

Target Data

Function Catalyzes the first and rate limiting step of the catabolism of the essential amino acid tryptophan along the kynurenine pathway. Involved in the peripheral immune tolerance, contributing to maintain homeostasis by preventing autoimmunity or immunopathology that would result from uncontrolled and overreacting immune responses. Tryptophan shortage inhibits T lymphocytes division and accumulation of tryptophan catabolites induces T-cell apoptosis and differentiation of regulatory T-cells. Acts as a suppressor of anti-tumor immunity
Gene References into Functions
  1. Influenza infection of NIH-3T3 cells elevates the expression of indoleamine 2,3 dioxygenase (IDO). Inhibition against IDO followed by infection increases the level of viral RNA and reduces the upregulation of 3-hydroxyanthranilate 3,4-dioxygenase driven by virus. Induction of IDO appears to contribute to limiting replication of the WSN/33 strain of influenza A virus in murine NIH-3T3 cells. PMID: 28402179
  2. Study showed that the knockout of IDO prevented vascular smooth muscle cells apoptosis in AngII -treated Ldlr-/- mice fed with HFD, suggesting a detrimental role of IDO in abdominal aortic aneurysm formation. PMID: 29494675
  3. The KYNurenine pathway of IDO1-mediated Tryptophan metabolism plays a critical role in depressive symptoms associated with IFN-alpha therapy. PMID: 27436416
  4. IDO is a critical regulator of acute pulmonary inflammation . PMID: 28673995
  5. Data suggest that Indoleamine 2,3-dioxygenase 1 (IDO1) appears to be a potential hallmark of liver lesions, and its deficiency protects mice from CCl4-induced fibrosis mediated by Th17 cells down-regulation and tryptophan 2,3-dioxygenase (TDO) compensatory increase. PMID: 28465467
  6. Indoleamine 2,3-dioxygenase regulates anti-tumor immunity in lung cancer by metabolic reprogramming of immune cells in the tumor microenvironment PMID: 27705910
  7. Findings suggest non-redundant neurophysiological roles for indoleamine 2,3-dioxygenase 1, indoleamine 2,3-dioxygenase 2 and tryptophan 2,3-dioxygenase in modulating brain activities and metabolism. PMID: 27316339
  8. These results show IDO is upregulated with RSV infection and this upregulation likely participates with IFN-gamma in inhibition of virus replication and suppression of some host cell responses to infection. PMID: 28963880
  9. Lipopolysaccharide (LPS) stimulation increased the expression and activity of the immunoregulatory enzyme IDO1 in hepatic stellate cells (HSCs), and LPS/HSCs stimulated aryl hydrocarbon receptor (AhR) signaling in cocultured regulatory T cells. PMID: 27581538
  10. this study shows that the presence of IFN-alpha at antigen sensitization activates an IDO1/TGF-beta-dependent anti-inflammatory program that upon antigenic rechallenge prevents inflammation via plasmacytoid dendritic cells PMID: 27647832
  11. The deficiency of indoleamine 2,3-dioxygenase aggravates the carbon tetrachloride-induced liver fibrosis in mice. PMID: 27598994
  12. Across strains, networks depicted a predominance of genes under-expressed in microglia relative to macrophages that may be a precursor for the different response of both cell types to challenges. The detected transcriptome differences enhance the understanding of the role of IDO1 in the microglia transcriptome under unchallenged conditions. PMID: 27314674
  13. Data show that indoleamine 23-dioxygenase 1 (IDO-1) inhibitors 1-methyl-D-tryptophan was able to alleviate most of the behavioural changes resulting from unpredictable chronic mild stress (UCMS) exposure. PMID: 27828964
  14. IDO did not play a pivotal role in the suppression of allergic airway inflammation through adipose-derived stem cells, suggesting that it is not the major regulator responsible for suppressing allergic airway inflammation. PMID: 27812173
  15. Aortic Plasmacytoid dendritic cells expressed CCR9 and indoleamine 2,3-dioxygenase 1 (IDO-1), an enzyme involved in driving the generation of regulatory T cells (Tregs). PMID: 27166946
  16. Indoleamine-2,3-dioxygenase (IDO) production by Plasmacytoid dendritic cells (pDCs)is necessary to confer suppressive function to T-Cells, Regulatory (Tregs) in experimental autoimmune encephalomyelitis (EAE). PMID: 27470005
  17. our findings support the hypothesis elevated IDO activity in non-CNS due to virus infections causes pain hypersensitivity PMID: 27168185
  18. this study shows that IDO overexpression in dendritic cells attenuates acute allograft rejection PMID: 27107370
  19. This insight into IDO1's involvement in pro-tumorigenic inflammatory neovascularization may have important ramifications for IDO1 inhibitor development, not only in cancer where clinical trials are currently ongoing, but in other disease indications associated with neovascularization as well. PMID: 27889479
  20. Inhibition of IDO activity ameliorated Japanese encephalitis via enhancement of antiviral IFN-I/II innate and adaptive T-cell responses and increased central nervous system infiltration of peripheral leukocytes. PMID: 27090635
  21. Results suggest that IDO expression is implicated in immunosuppression and tumor progression in glioma cells; combining IDO inhibition with standard TMZ treatment could be an encouraging therapeutic strategy for patients with malignant glioma PMID: 26636389
  22. Data show that the expression of indoleamine 2, 3-dioxygenase 1 (IDO) was decreased after tumor cells were infected with Salmonella. PMID: 26517244
  23. Severity of sodium dodecyl sulfate-induced colitis is reduced in Ido1-deficient mice with down-regulation of TLR-MyD88-NF-kB transcriptional networks. PMID: 26610689
  24. Data implicate indoleamine 2,3-dioxygenase-dependent neurotoxic kynurenine metabolism as a pathogenic factor for cognitive dysfunction in inflammation-induced depressive disorders and a potential novel target for the treatment of these disorders. PMID: 26130057
  25. IDO1 deficiency does not affect inflammation in Systemic Juvenile Idiopathic Arthritis, Secondary Hemophagocytic Lymphohistiocytosis and a T cell-triggered cytokine release model. PMID: 26914138
  26. Increased expression of IDO in liver cell adenomas compared to the surrounding normal tissue may create a microenvironment that promotes the progression of HCC by suppressing the proliferation of cytotoxic T lymphocytes and enhancing Tregs. PMID: 26727596
  27. Chimeric vaccine stimulation of human dendritic cell IDO1 occurs via the non-canonical NF-kappaB pathway. PMID: 26881431
  28. Absence of Ido1 protects against atherosclerosis through increase of Il10. PMID: 26235422
  29. Indoleamine 2,3-Dioxygenase Is Involved in the Inflammation Response of Corneal Epithelial Cells to Aspergillus fumigatus Infections PMID: 26361229
  30. the role of IFN-lambda in IDO regulation was investigated after influenza infection of respiratory epithelial cells. PMID: 25756191
  31. TNF-alpha mediates stress-induced depression by upregulating indoleamine 2,3-dioxygenase in a mouse model of unpredictable chronic mild stress. PMID: 26083579
  32. Experimental hemophilic mouse models with or without functional IDO1 revealed that tryptophan metabolites, which result from IDO1 activity, prevent generation of anti-FVIII antibodies. PMID: 26426076
  33. Data indicate indoleamine 23-dioxygenase 1 IDO1 induction in B cells as a negative regulatory mechanism of the T Cell-independent antigens (TI) humoral immune response. PMID: 26216892
  34. IFN-gamma coordinately induces IDO1 and a tryptophan-selective transporter in human colonic epithelial cells and mouse dendritic cells with a positive feedback mechanism via kynurenine-AhR signaling. PMID: 25450809
  35. The data showed that there is not significant effect of IDO1 or TDO2 on mortality in pneumococcal meningitis. PMID: 24844751
  36. dendritic cell-based immune response mediated by interferon-gamma-induced IDO expression via GSK-3beta activity not only regulates CD8(+) T-cell proliferation and cytotoxic T lymphocyte activity but also modulates OVA-pulsed DC vaccination against EG7 thymoma PMID: 25814664
  37. Data suggest that transferred TGF-beta-induced regulatory T cells (iTregs) could induce tolerogenic splenic dendritic cells and these cells could effectively dampen collagen-induced arthritis in an indoleamine 2,3-dioxygenase (IDO)-dependent manner. PMID: 25405209
  38. 20 weeks of Western diet altered LPS-induced depressive-like behavior compared to LPS-treated lean mice and exacerbated hippocampal and hypothalamic proinflammatory cytokine expression and brain IDO activation. PMID: 24681251
  39. B7-2 costimulation and intracellular indoleamine 2,3-dioxygenase expression is reduced in umbilical cord blood as compared to adult peripheral blood. PMID: 24930629
  40. These data indicate that activation of brain IDO1 is sufficient to induce depression-like behaviors of mice in response to central LPS. PMID: 23866724
  41. IDO2 is critical for IDO1-mediated T-cell regulation and exerts a non-redundant function in inflammation. PMID: 24402311
  42. Inhibitors were used to clarify the role of IDO in graft-vs-tumor reactions after allogeneic stem cell transplantation plus donor leukocyte infusion. IDO1 expression in tumor tissues and TDLN, but not spleen, was increased in the mice receiving DLI. PMID: 24971697
  43. The role of IDO during initial host response to influenza infection was studied by using a specific inhibitor. IDO inhibition enhanced lung proinflammatory cytokine gene and protein expression at 24 and 48 h post influenza virus infection. PMID: 24799604
  44. engagement of CD80/86 by CTLA-4 induced activation of the enzyme indoleamine 2,3-dioxygenase (IDO) in osteoclast precursors, which degraded tryptophan and promoted apoptosis PMID: 24807557
  45. The study indicates that IDO1 is spatiotemporally expressed in activated microglia during acute viral encephalitis by encephalomyocarditis virus. PMID: 24530381
  46. Systemic primary and recall T-cell CD8 responses to viral antigens are controlled by IDO. PMID: 24587363
  47. tumor-derived IDO promotes the peritoneal dissemination of ovarian cancer through creating an immunotolerogenic environment within the peritoneal cavity PMID: 24826982
  48. Induction of hepatitis B virus surface antigen-specific cytotoxic T lymphocytes can be up-regulated by the inhibition of indoleamine 2, 3-dioxygenase activity. PMID: 24580128
  49. Data indicate that the frequency and absolute number of Treg cells increased in indoleamine 2,3-dioxygenase (IDO) expressing fibroblast environment PMID: 23891282
  50. we address this issue with the development of IDO1 monoclonal antibody 4B7 which specifically recognizes the murine enzyme in tissue sections, offering a reliable tool for immunohistology in preclinical disease models. PMID: 24123235

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Subcellular Location Cytoplasm, cytosol
Protein Families Indoleamine 2,3-dioxygenase family
Tissue Specificity Highly expressed in epididymis, duodemum, jejunum, ileum, colon and spleen (PubMed:19741271). Highly expressed in epididymis, prostate, duodemum, jejunum, ileum, colon and spleen, not detected in the liver (at protein level) (PubMed:19741271). Expressed i
Database Links

KEGG: mmu:15930

STRING: 10090.ENSMUSP00000033956

UniGene: Mm.392

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