Mouse Insulin-like growth factor-binding protein complex acid labile subunit(IGFALS) ELISA kit

Code CSB-EL011094MO
Size 96T,5×96T,10×96T
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Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
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Product Details

Target Name
insulin-like growth factor binding protein, acid labile subunit
Alternative Names
Igfals ELISA Kit; Albs ELISA Kit; AlsInsulin-like growth factor-binding protein complex acid labile subunit ELISA Kit; ALS ELISA Kit
Abbreviation
IGFALS
Uniprot No.
Species
Mus musculus (Mouse)
Sample Types
serum, plasma, tissue homogenates
Detection Range
3.12 ng/mL-200 ng/mL
Sensitivity
0.78 ng/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Others
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse IGFALS in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:100Average %96
Range %93-99
1:200Average %84
Range %80-87
1:400Average %91
Range %87-94
1:800Average %107
Range %104-110
Recovery
The recovery of mouse IGFALS spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9490-98
EDTA plasma (n=4)8581-89
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
2001.985 1.901 1.943 1.838
1001.543 1.578 1.561 1.456
501.055 1.064 1.060 0.955
250.657 0.648 0.653 0.548
12.50.346 0.352 0.349 0.244
6.250.248 0.268 0.258 0.153
3.120.191 0.193 0.192 0.087
00.106 0.104 0.105
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Mouse IGFALS ELISA Kit was designed for the quantitative measurement of Mouse IGFALS protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 3.12 ng/mL-200 ng/mL and the sensitivity is 0.78 ng/mL.

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Target Background

Function
(From Uniprot)
May have an important role in regulating the access of circulating IGFs to the tissues.
Gene References into Functions
  1. the Igfals gene has a role in skeletal response of male mice to anabolic hormone therapy PMID: 24424061
  2. A modest reduction in post-natal growth in the null ALS mice and in the ALS-deficient patients was observed PMID: 16114275
Subcellular Location
Secreted, extracellular space.
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