Mouse Insulin-like growth factor-binding protein complex acid labile subunit(IGFALS) ELISA kit

Code CSB-EL011094MO
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Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. P70389
Lead Time 5-7 working days
Abbreviation IGFALS
Protein Biological Process 1 Cell Adhesion
Target Name insulin-like growth factor binding protein, acid labile subunit
Alias ALS, insulin-like growth factor binding protein complex acid labile chain
Species Mus musculus (Mouse)
Protein Biological Process 3 Cell adhesion
Sample Types serum, plasma, tissue homogenates
Detection Range 3.12 ng/mL-200 ng/mL
Sensitivity 0.78 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Others
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details This protein is a serum protein that binds insulin-like growth factors, increasing their half-life and their vascular localization. Production of the encoded protein, which contains twenty leucine-rich repeats, is stimulated by growth hormone. Defects in this gene are a cause of acid-labile subunit deficiency, which maifests itself in a delayed and slow puberty. Three transcript variants encoding two different isoforms have been found for this gene.
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse IGFALS in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:100Average %96
Range %93-99
1:200Average %84
Range %80-87
1:400Average %91
Range %87-94
1:800Average %107
Range %104-110
Recovery
The recovery of mouse IGFALS spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9490-98
EDTA plasma (n=4)8581-89
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
2001.985 1.901 1.943 1.838
1001.543 1.578 1.561 1.456
501.055 1.064 1.060 0.955
250.657 0.648 0.653 0.548
12.50.346 0.352 0.349 0.244
6.250.248 0.268 0.258 0.153
3.120.191 0.193 0.192 0.087
00.106 0.104 0.105
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Function May have an important role in regulating the access of circulating IGFs to the tissues.
Subcellular Location Secreted, extracellular space
Database Links

KEGG: mmu:16005

STRING: 10090.ENSMUSP00000060169

UniGene: Mm.3135

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